12 documents found, page 1 of 2
Clinical validation of an in-house quantitative real time PCR assay for cytomeg...
Caurio,Cassia F.B.; Allende,Odelta S.; Kist,Roger; Santos,Kênya L.; Vasconcellos,Izadora C.S.; Rozales,Franciéli P.; Lana,Daiane F. DallaAbstract Introduction: Cytomegalovirus (CMV) is one of the most common agents of infection in solid organ transplant patients, with significant morbidity and mortality. Objective: This study aimed to establish a threshold for initiation of preemptive treatment. In addition, the study compared the performance of antigenemia with qPCR results. Study design: This was a prospective cohort study conducted in 2017 in...
Confirmed Invasive Pulmonary Aspergillosis and COVID-19: the value of postmorte...
Santana,Monique Freire; Pivoto,Guilherme; Alexandre,Márcia A. Araujo; Baía-da-Silva,Djane Clarys; Borba,Mayla Gabriela da Silva; Val,Fernando AlmeidaAbstract We present postmortem evidence of invasive pulmonary aspergillosis (IPA) in a patient with severe COVID-19. Autopsies of COVID-19 confirmed cases were performed. The patient died despite antimicrobials, mechanical ventilation, and vasopressor support. Histopathology and peripheral blood galactomannan antigen testing confirmed IPA. Aspergillus penicillioides infection was confirmed by nucleotide sequenc...
Cost minimization analysis of an in-house molecular test for cytomegalovirus in...
Caurio,Cássia F.B.; Allende,Odelta S.; Kist,Roger; Vasconcellos,Izadora C.S.; Rozales,Francieli P.; Reck-Kortmann,Maikel; Dalla Lana,Daiane F.ABSTRACT Introduction: Cytomegalovirus may cause severe disease in immunocompromised patients. Nowadays, quantitative polymerase chain reaction is the gold-standard for both diagnosis and monitoring of cytomegalovirus infection. Most of these assays use cytomegalovirus automated molecular kits which are expensive and therefore not an option for small laboratories, particularly in the developing world. Objective...
Top 10 evidence-based recommendations from the Brazilian Society of Infectious ...
Pasqualotto,Alessandro C.; Almeida,Camila S.; Kliemann,Dimas A.; Barcellos,Guilherme B.; Queiroz-Telles,Flávio; Abdala,Edson; Resende,MariangelaABSTRACT The Choosing Wisely Initiative aims to collect statements from medical societies all over the world on medical interventions that result in no benefit to patients, with the potential to cause harm. In this article we present the views of the Diagnostic Laboratory Group at the Brazilian Society of Infectious Diseases (SBI). Ten experts from SBI were asked to list 10 diagnostic tests that were perceived ...
Quantitative detection of BK virus in kidney transplant recipients: a prospecti...
Pinto,Gabriel Godinho; Poloni,José Antonio Tesser; Paskulin,Diego D'Avila; Spuldaro,Fabio; Paris,Fernanda de; Barth,Afonso Luís; Manfro,Roberto CerattiAbstract Introduction: BK virus (BKV) infection in renal transplant patients may cause kidney allograft dysfunction and graft loss. Accurate determination of BKV viral load is critical to prevent BKV-associated nephropathy (BKVAN) but the cut-off that best predicts BKVAN remains controversial. Objective: To evaluate the performance of a commercial and an in-house qPCR test for quantitative detection of BK virus...
Galactomannan use in clinical practice: providing free testing is not the full ...
dos Santos,Jeniffer S.; Hermes,Djuli M.; Pasqualotto,Alessandro C.ABSTRACT Introduction: Invasive aspergillosis is a condition associated with a high mortality rate mostly due to difficulties in performing an early diagnosis. In recent years, galactomannan detection has markedly improved the diagnosis of invasive aspergillosis, but very little is known on how physicians deal with this test in clinical practice. Methods: This cross-sectional study aimed to analyze the indicati...
Progressive disseminated histoplasmosis: a systematic review on the performance...
Falci,Diego R.; Hoffmann,Elias R.; Paskulin,Diego D.; Pasqualotto,Alessandro C.Abstract The diagnosis of progressive disseminated histoplasmosis is often a challenge to clinicians, especially due to the low sensitivity and long turnaround time of the classic diagnostic methods. In recent years, studies involving a variety of non-culture-based diagnostic tests have been published in the literature. We performed a systematic review by selecting studies evaluating non-culture-based diagnosti...
Screening for BK virus nephropathy in kidney transplant recipients: comparison ...
Pinto,Gabriel Godinho; Poloni,Jose Antonio T; Rotta,Liane N.; Razonable,Raymund R.; Pasqualotto,Alessandro C.Abstract Urine cytology and qPCR in blood and urine are commonly used to screen renal transplant recipients for polyomavirus-associated nephropathy (PVAN). Few studies, however, have directly compared these two diagnostic tests, in terms of their performance to predict PVAN. This was a systematic review in which adult (≥ 18 years old) renal transplant recipients were studied. A structured Pubmed search was used...
Clinical, laboratorial and radiographic predictors of Bordetella pertussis infe...
Bellettini,Camila Vieira; Oliveira,Andressa Welter de; Tusset,Cintia; Baethgen,Ludmila Fiorenzano; Amantéa,Sérgio Luís; Motta,Fabrizio; Gasparotto,AlineOBJECTIVE: To identify clinical, laboratorial and radiographic predictors for Bordetella pertussis infection.METHODS: This was a retrospective study, which analyzed medical records of all patients submitted to a molecular dignosis (qPCR) for B. pertussis from September 2011 to January 2013. Clinical and laboratorial data were reviewed, including information about age, sex, signs/symptoms, length of hospitalizat...
Ultra-performance liquid chromatographic method for measurement of voriconazole...
Boeira,Paula; Antunes,Marina V.; Andreolla,Huander F.; Pasqualotto,Alessandro C.; Linden,RafaelA simple, sensitive and selective ultra-performance liquid chromatography method for the determination of voriconazole in plasma and oral fluid was developed and validated. After a liquid-liquid extraction with methyl-tert-butyl ether, the analyte and internal standard were separated on a Hypersil Gold C18 column (2.1 × 100 mm, p.d. 1.9 µm), eluted with a mobile phase composed of thietylammonium phosphate buffe...