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Sensors in the Detection of Abused Substances in Forensic Contexts: A Comprehen...
Rosendo, Luana M.; Antunes, Mónica; Simão, Ana Y.; Brinca, Ana Teresa; Catarro, Gonçalo; Pelixo, Rodrigo; Martinho, João; Pires, Bruno; Soares, Sofiaorensic toxicology plays a pivotal role in elucidating the presence of drugs of abuse in both biological and solid samples, thereby aiding criminal investigations and public health initiatives. This review article explores the significance of sensor technologies in this field, focusing on diverse applications and their impact on the determination of drug abuse markers. This manuscript intends to review the tran...
Desenvolvimento e validação de um método para a determinação de canabinóides em...
Rosendo, Luana; Rosado, Tiago; Oliveira, Patrik; Simão, Ana; Margalho, Cláudia; Costa, Suzel; Passarinha, Luís; Gallardo, Eugenia; Barroso, MárioDe acordo com o Observatório Europeu da Droga e da Toxicodependência, no último ano (dados de junho de 2022), existiam aproximadamente 22,2 milhões consumidores de canábis na Europa. A urina é uma das amostras mais utilizadas para a deteção e quantificação de canabinóides. (...)
Determinação de canabinóides em amostras de urina por microextração em seringa ...
Rosendo, Luana; Rosado, Tiago; Oliveira, Patrik; Antunes, Cal; Anjos, O; Margalho, Cláudia; Costa, Suzel; Passarinha, Luís; Barroso, MárioA canábis é a substância ilícita mais frequentemente consumida a nível mundial. Em laboratórios de análise de drogas. A urina é uma das amostras mais utilizadas para a deteção de canabinóides. Nesta matriz biológica é possí -tretahidrocanabinol (THC), principal composto psicoativo presente na cannabis, o ácido 11-nor-9-carboxi- -tretahidrocanabinol (THC-COOH) e ainda o 11-hidroxi- -tetrahydrocannabiol (11-OH-TC...
In Silico Approaches: A Way to Unveil Novel Therapeutic Drugs for Cervical Canc...
Gomes, Diana; Silvestre, Samuel; Duarte, Ana Paula; Venuti, Aldo; Soares, Christiane P.; Passarinha, Luís; Sousa, ÂngelaCervical cancer (CC) is the fourth most common pathology in women worldwide and presents a high impact in developing countries due to limited financial resources as well as difficulties in monitoring and access to health services. Human papillomavirus (HPV) is the leading cause of CC, and despite the approval of prophylactic vaccines, there is no effective treatment for patients with pre-existing infections or ...
Purification of interferon alpha 2b-based biopharmaceuticals using ionic liquid...
Castro, Leonor; Lobo, Guilherme; Pereira, Patrícia; Passarinha, Luís; Neves, Márcia C.; Freire, Mara G.; Pedro, Augusto Q.Protein biopharmaceuticals, among which interferon alpha-2b (IFNα-2b) that can be used in the treatment of chronic hepatitis C, have become an indispensable product of current medicine. Aiming at finding new cost-effective, efficient and sustainable technologies for recombinant IFNα-2b purification, ionic liquids were investigated as adjuvants in polymer-polymer aqueous two-phase systems (ATPS) or as chromatogr...
Enhanced performance of polymer-polymer aqueous two-phase systems using ionic l...
Castro, Leonor; Pereira, Patrícia; Passarinha, Luís; Freire, Mara; Pedro, AugustoProtein biopharmaceuticals, among which interferon alpha-2b (IFNα-2b) that can be used in the treatment of chronic hepatitis C and hairy cell leukemia, have become an indispensable product of current medicine. However, their current high costs derived from the lack of cost-effective downstream strategies still limits their widespread use. Polymer-based aqueous two-phase systems (ATPS) or aqueous biphasic system...
Aqueous two-phase systems comprising ionic liquids for the extraction of recomb...
Castro, Leonor S.; Almeida, Mafalda R.; Pereira, Patrícia; Passarinha, Luís; Sousa, Fani; Coutinho, João; Tavares, Ana P. M.; Freire, Mara G.The progress made on the use of new biopharmaceuticals has a high influence on the global health, and since the approval of humulin (recombinant insulin) by FDA, new protein-based pharmaceuticals have been introduced in the market such as the interferon alfa 2b (IFNα2b), which has been used in in the treatment of oncological diseases for more than 30 years. Nowadays, the (bio)pharmaceutical industry is committe...
Improving the purification of recombinant proteins by the use of ionic liquids ...
Castro, Leonor S.; Almeida, Mafalda R.; Pereira, Patrícia; Passarinha, Luís; Sousa, Fani; Coutinho, João A. P.; Tavares, Ana P. M.; Pedro, Augusto Q.The development of new drugs has a high impact on the global health, and since the approval of humulin (recombinant insulin) by FDA, several protein-based biopharmaceuticals have been introduced in the market. These products are usually attained through biotechnological processes using engineered biological sources and have led to remarkable advances in the treatment of distinct human diseases, as for example t...
Bioprocessing of recombinant proteins using alternative purification platforms
Castro, Leonor S.; Almeida, Mafalda R.; Pereira, Patrícia; Passarinha, Luís; Tavares, Ana P. M.; Pedro, Augusto Q.; Freire, Mara G.The advent of biopharmaceuticals in modern medicine brought enormous benefits to diverse human diseases and improved the well-being of many people worldwide. Since the FDA approval of humulin (recombinant insulin) in 19821, remarkable advances in the treatment of chronic diseases have been achieved with biopharmaceutical-based therapies2. This sector represents 40% of ca. 6000 products currently in clinical dev...
Downstream processing of recombinant proteins using alternative purification pl...
Castro, Leonor S.; Almeida, Mafalda R.; Pereira, Patrícia; Passarinha, Luís; Tavares, Ana P. M.; Coutinho, João A. P.; Pedro, Augusto Q.Remarkable advances in the treatment of chronic diseases have been achieved with biopharmaceutical-based therapies, a sector that is currently dominated by therapeutic proteins with over 200 protein drugs in the market1. Improvements in the biopharmaceuticals upstream stage have resulted in high yields of the desired product, and shifted the bioprocess bottleneck to the downstream processing, which is currently...