ABSTRACT: A methodology was developed to assess the allocation of different types of endogenous waste biomass to eight technologies for producing electricity, heat, biogas and advanced biofuels. It was based on the identification of key physicochemical parameters for each conversion process and the definition of limit values for each parameter, applied to two different matrices of waste biomass. This enabled th...
Executive Summary: There is a clear need to accelerate the energy transition, including the implementation of renewable electricity production plants, as well as the increase in consumption of other renewable energy carriers in buildings, industry, transport and other sectors. This work provides key information to make this transition possible, that is, the technical renewable energy potentials for Portugal. Th...
ABSTRACT: In the scope of the CONVERTE project an optimised and uniformed methodology was developed in order to better evaluate the use of endogenous biomasses for energy production by biochemical or thermochemical processes. A protocol for preparation and characterization was designed and validated by the use of biomass certified reference materials and quality control actions. Extensive analytical work was pe...
ABSTRACT: Flow cytometry was used to evaluate the effect of initial ethanol concentrations on cyanobacterial strains of Synechocystis PCC 6803 [wild-type (WT), and ethanol producing recombinants (UL 004 and UL 030)] in batch cultures. Ethanol recombinants, containing one or two metabolically engineered cassettes, were designed towards the development of an economically competitive process for the direct product...
ABSTRACT: A new process for co-extraction and separation of fatty acids and carotenoids from Rhodosporidium toruloides NCYC 921 biomass in order to achieve full exploitation of the yeast lipidic fraction is described. A saponification of the wet yeast biomass was performed using a potassium hydroxide solution (1.1 M) in ethanol 96%, at 65 °C for 180 min. In the carotenoid extraction step, a biphasic system with...
Single-cell oils (SCO) have been considered a promising source of 3rd generation biofuels mainly in the final form of biodiesel. However, its high production costs have been a barrier towards the commercialization of this commodity. The fast growing yeast Rhodosporidium toruloides NCYC 921 has been widely reported as a potential SCO producing yeast. In addition to its well-known high lipid content (that can be ...
Microalgae have a wide range of application fields, from food to fuels, to pharmaceuticals & fine chemicals, aquaculture and environmental bioremediation, among others. Spirulina and Chlorella have been used as food sources since ancient times, due to their high and balanced nutritional value. Our research group in Lisbon has developed a range of food products (emulsions, gelled desserts, biscuits and pastas) e...
The use of lignocellulosic materials as substrate for bioethanol production is considered a cost-effective approach to make the biofuel production process economically sustainable. However, lignocellulosic hydrolysis releases toxic compounds such as weak acids which inhibit microorganism growth and ethanol production. In order to understand the physiological response of Saccharomyces carlsbergensis when ferment...
The aim of the project BIOFFA is to develop processes for the production of biofuels from residual raw-materials with high free fatty acid (FFA). In technological terms, two distinct approaches, leading to different final products, are being assessed: production of fatty acid methyl esters (FAME) – biodiesel, and hydrogenated oil – H-oil. Different residues available in Portugal, including poultry fat, cattle f...