11 documents found, page 1 of 2
Extracellular vesicles in infectious diseases caused by protozoan parasites in ...
Pontes,Leticia Gomes de; Altei,Wanessa Fernanda; Galan,Asier; Bilić,Petra; Guillemin,Nicolas; Kuleš,Josipa; Horvatić,AnitaAbstract Background: Extracellular vesicles (EVs) are small membrane-bound vesicles of growing interest in vetetinary parasitology. The aim of the present report was to provide the first isolation, quantification and protein characterization of EVs from buffalo (Bubalus bubalis) sera infected with Theileria spp. Methods: Infected animals were identified through optical microscopy and PCR. EVs were isolated from...
Desenvolvimento e caracterização de nanocápsulas de poli (L-lactídeo) contendo ...
Melo,Nathalie Ferreira Silva de; Grillo,Renato; Rosa,André Henrique; Fraceto,Leonardo Fernandes; Dias Filho,Newton Luiz; Paula,Eneida deIn this paper we describe the preparation poly (L-lactide) (PLA) nanocapsules as a drug delivery system for the local anesthetic benzocaine. The characterization and in vitro release properties of the system were investigated. The characterization results showed a polydispersity index of 0.14, an average diameter of 190.1± 3 nm, zeta potential of -38.5 mV and an entrapment efficiency of 73%. The release profile...
Physicochemical stability of poly(lactide-co-glycolide) nanocapsules containing...
Moraes,Carolina M.; Paula,Eneida de; Rosa,André H.; Fraceto,Leonardo F.This paper describes the preparation of poly(DL-lactide-co-glicolide) (PLGA) nanocapsules as a drug carrier system for the local anesthetic bupivacaine. The system was characterized and its stability investigated. The results showed a size distribution with a polydispersity index of 0.12, an average diameter of 148 nm, a zeta potential of -43.5 mV and an entrapment efficiency of 75.8%. The physicochemical prope...
Pharmacokinetic profile of liposome-encapsulated ropivacaine after maxillary in...
Franz-Montan,Michelle; Bergamaschi,Cristiane de Cássia; Paula,Eneida de; Groppo,Francisco C.; Ranali,José; Fraceto,Leonardo Fernandes; Volpato,Maria C.The aim of this study was to determine the pharmacokinetic parameters of liposomal ropivacaine after dental anesthesia in 14 healthy volunteers. In this randomized, double-blind and crossover study, the volunteers received maxillary infiltration of liposome-encapsulated 0.5% ropivacaine and, 0.5% ropivacaine with 1:200,000 epinephrine in two different sessions. Blood samples were collected before and after (fro...
Anestésicos locais: interação com membranas biológicas e com o canal de sódio v...
Araujo,Daniele Ribeiro de; Paula,Eneida de; Fraceto,Leonardo FernandesMany theories about the mechanism of action of local anesthetics (LA) are described in the literature. Two types of theories can be distinguished: those that focus on the direct effects of LA on their target protein in the axon membranes, i.e. the voltage-gated sodium channel and the ones that take into account the interaction of anesthetic molecules with the lipid membrane phase for the reversible nerve blocka...
Validação de metodologia analítica por cromatografia líquida de alta eficiência...
Moraes,Carolina Morales; Paula,Eneida de; Rosa,André Henrique; Fraceto,Leonardo FernandesBupivacaine (S75-R25, NovaBupi®) is an amide type local anesthetic widely used. The present work consists of the development and validation of analytical methodology for evaluation of NovaBupi® content in the poly-lactide-co-glycolide nanospheres (PLGA-NS) by high performance liquid chromatography. The separation was made using the reversed-phase column LC-18, acetonitrile/phosphate buffer 85:15 v/v as mobile p...
Caracterização do complexo de inclusão ropivacaína: beta-ciclodextrina
Fraceto,Leonardo Fernandes; Gonçalves,Marcos Moisés; Moraes,Carolina Morales; Araújo,Daniele Ribeiro de; Zanella,Luciana; Paula,Eneida deCharacteriza of the inclusion complex ropivacaine: beta-cyclodextrin. Ropivacaine (RVC) is a widely used local anesthetic. The complexation of RVC with beta-cyclodextrin (beta-CD) is of great interest for the development of more efficient local anesthetic formulations. The present work focuses on the characterization of the RVC:beta-CD complex by nuclear magnetic resonance (NMR). The stoichiometry of the comple...
Mistura com excesso enantiomérico de 50% (S75-R25) de bupivacaína complexada co...
Araujo,Daniele Ribeiro de; Braga,Angélica de Fátima de Assunção; Moraes,Carolina Morales; Fraceto,Leonardo Fernandes; Paula,Eneida deJUSTIFICATIVA E OBJETIVOS: Com a finalidade de prolongar a duração de ação e reduzir a toxicidade sistêmica, têm-se desenvolvido formulações de anestésicos locais (AL) complexados com ciclodextrinas (CD). Este estudo realizou a caracterização físico-química e avaliou, em ratos, os efeitos dos complexos de inclusão de bupivacaína racêmica (S50-R50) e da mistura com excesso enantiomérico de 50% (S75-R25) de bupiv...
Sistemas de liberação controlada com bupivacaína racêmica (S50-R50) e mistura e...
Araújo,Daniele Ribeiro de; Fraceto,Leonardo Fernandes; Braga,Angélica de Fátima de Assunção; Paula,Eneida deJUSTIFICATIVA E OBJETIVOS: Os efeitos adversos associados ao uso de bupivacaína levaram à procura por novos anestésicos locais (AL) com perfil de bloqueio semelhante e menos tóxicos, surgindo novas preparações como a mistura enantiomérica de bupivacaína (S75-R25). Os sistemas de liberação controlada, contendo AL em carreadores como ciclodextrinas (CD), têm como objetivo melhorar a eficácia anestésica e o índice...
Anestésicos locais: interação com membranas de eritrócitos de sangue humano, es...
Fraceto,Leonardo Fernandes; Paula,Eneida deThe literature carries many theories about the mechanism of action of local anesthetics (LA). We can highlight those focusing the direct effect of LA on the sodium channel protein and the ones that consider the interaction of anesthetic molecules with the lipid membrane phase. The interaction between local anesthetics and human erythrocyte membranes has been studied by ¹H and 31P nuclear magnetic resonance spec...