This study aimed to validate the estimates of the ruminal degradation of total carbohydrates (TC), ruminal and total digestion of fibrous carbohydrates (FC) and microbial nitrogen flow in the abomasum evaluated by in vitro gas production technique (IVGP). Six ruminally and abomasally cannulated steers arranged in a double 3 × 3 latin square were used to measure described parameters with indigestible neutral det...