3 documents found, page 1 of 1
A Polymeric Nanoparticle Formulation for Targeted mRNA Delivery to Fibroblasts
Rodrigues, Artur Filipe; Rebelo, Catarina; Simões, Susana; Paulo, Cristiana; Pinho, Sónia A.; Francisco, Vitor; Ferreira, LinoMessenger RNA (mRNA)-based therapies offer enhanced control over the production of therapeutic proteins for many diseases. Their clinical implementation warrants formulations capable of delivering them safely and effectively to target sites. Owing to their chemical versatility, polymeric nanoparticles can be designed by combinatorial synthesis of different ionizable, cationic, and aromatic moieties to modulate ...
A fluorescence based sensor assay that monitors general protein aggregation in ...
Pereira, Marisa; Tomé, Diogo; Domingues, Ana S.; Varanda, Ana S.; Paulo, Cristiana; Santos, Manuel A. S.; Soares, Ana R.Protein conformational disorders are characterized by disruption of protein folding and toxic accumulation of protein aggregates. Here we describe a sensitive and simple method to follow and monitor general protein aggregation in human cells. Heat shock protein 27 (HSP27) is an oligomeric small heat shock protein that binds and keeps unfolded proteins in a folding competent state. This high specificity of HSP27...
Retrospective analysis of clinical yeast isolates in a hospital in the centre o...
Paulo, Cristiana; Mourão, Cristina; Veiga, Pedro M.; Marques, Joana M.; Rocha, Graça; Alves, Ana F.; Querol, Amparo; Meliço-Silvestre, António A.We conducted a four-year (2003-2006) retrospective study of yeasts recovered in a hospital laboratory in the centre of Portugal to evaluate the epidemiology of yeast infections. Clinical isolates and data were gathered from 751 patients corresponding to 906 episodes of yeast infection. The isolates were first identified using classical and commercial methods, routinely employed at the hospital laboratory. We th...