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Macro and microscopic comparison of the upper pole of the spleen with the lower...
Paulo,Lucas Nagib Lemos; Alexander,Daniel Robert; Vallandro,Elisa Ines Demuner; Benevides,Raquel de Azevedo; Motta,Luciene Lage daAbstract Purpose To evaluate the viability of the upper (UP) and lower pole (LP) of the spleen from a macro and microscopic point of view, after subtotal splenectomy with preservation (SSP) of the UP and the LP. Methods Seventeen male Wistar rats, two months old, were submitted to SSPUP and SSPLP and 5 to simulated operation (SG). After 80 days, the rats were euthanized, and the remaining LP and UP and intact s...
The influence of splenic tissue on the survival and TCD4 and TCD8 lymphocyte ra...
Borjaili,Andréa Saade Daher; Nunes,Giseli Celestino; Silveira,Julia Belizário; Dall'Orto,Flávia Heiderich; Lorenzoni,Gabriel SouzaAbstract Purpose: Comparing survival rates of rats subjected to spleen procedures after fecal peritonitis induction. Assessing changes in TCD4 and CD8 lymphocyte rates before and after the procedures. Correlating animal survival with CD4 and CD8 lymphocytes. Methods: Thirty male Wistar rats were distributed into 3 groups of ten: spleen manipulation (SM); total splenectomy (TS); subtotal splenectomy with preserv...
Micronucleus test in peripheral blood of rats treated with hyperbaric oxygen af...
Paulo,Marcela Souza Lima; Souza,Ingryd Fortes; Wandekoken,Kethleen Gomes; Silva,Karina Balestreiro; Dutra,Jean Carlos VencioneckPURPOSE: To assess the mutagenic potential of the oxygen inhalation therapy (HBO), by means of the micronucleus test, performed in peripheral blood of rats that underwent subtotal splenectomy with lower pole preservation (ESTPI), after HBO sessions or simulations.METHODS: Eighteen male Wistar rats, were distributed into three groups of six animals: group 1 - submitted to ESTPI and HBO sessions; group 2 - submit...
Effect of total splenectomy in the lipid profile in mice
Demuner,Bianca Lacerda; Pinho,Gotardo Zini; Thomaz,João Cunha; Stegmiller,Nataly Pescinalli; Mendes,Roberta Miranda de Araújo; Paulo,Marcela Souza LimaPURPOSE: To analyze total splenectomy effect on the lipid profile - total cholesterol, low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), very-low-density lipoprotein cholesterol (VLDL) and triglycerides levels, in Balb/c mice.METHODS: Thirty Balb/c male mice, one (1) month old and average weight 26.2g ± 4.0 were used in the experiment. They were distributed into three groups...
Effects of inferior splenic lobe pole fixation and gastrosplenic peritoneal mem...
Mendonça,Fernanda Alves; Carmo,Felipe Poubel Timm do; Paris,Louise Goncalves; Pagotte,Marya Duarte; Pereira,Fernanda Lube AntunesPURPOSE: To investigate the vitality of the spleen lower pole after subtotal splenectomy with suture to the stomach and after posterior peritoneal gastro-splenic membrane section, using macro and microscopic evaluations. METHODS: Sixty Wistar rats were used in this study and were randomly distributed in the three groups: Group 1: (n=20), subtotal splenectomy with lower pole preservation, Group 2: (n=20) subtota...
Epidural anesthesia with ropivacaine with or without clonidine and postoperativ...
Baptista,João Florêncio de Abreu; Gomez,Renato Santiago; Paulo,Danilo Nagib Salomão; Carraretto,Antonio Roberto; Brocco,Marcos Celio; Silva,José JorgePURPOSE: To determine the safety, pain intensity correlated with age and body mass index (BMI), epidural anesthesia with ropivacaine and clonidine in hemorrhoidectomy.METHODS: Eighty patients, both genders, 20-70 years old, ASA I or II, for hemorrhoidectomy were randomly divided into two groups: Control (n=38), epidural anesthesia with 14 mL of ropivacaine 0.75 % plus 0.0266 mL/kg of 0.9% saline solution; Exper...
Effects of human chorionic gonadotropin on the normal testicular tissue of rats
Altoé,Patricia Magnago; Tatsuo,Edson Samesima; Paulo,Danilo Nagib Salomão; Jarske,Robson; Milagres,Marcel; Loureiro,Igor DanielPURPOSE: To investigate the effects of human chorionic gonadotropin (hCG) on the testicular tissue of young male rats.METHODS: Male Wistar rats were assigned to groups (10 rats/group).Control Group received subcutaneous saline solution; Group 1 received hCG 50UI/Kg/dose; and Group 2 received hCG 100UI/Kg/dose, daily for 15 days. Half was submitted to bilateral orchiectomy on the 16th day and the other half 45 d...
Phagocytic function of lower spleen pole and autogenous splenic implants in rats
Borjaili,Andréa Saade Daher; Cerqueira,Brunela Silva; Silbermann,Julio Roitman; Rezende,Caroline Ferraz; Freire Júnior,David DomingosPURPOSE: To investigate whether there are differences between the phagocytic function of the remaining lower spleen pole after subtotal splenectomy and autogenous splenic implants. METHODS: Thirty-six male Wistar rats, weighting 364 ± 60g were used. They were subjected to subtotal splenectomy preserving the lower spleen pole and to autogenous splenic implant in the greater omentum. Its viability was assessed mi...
Decompressive fasciotomy in testicular torsion of the spermatic cord in rats
Quintaes,Isabela Passos Pereira; Tatsuo,Edson Samesima; Paulo,Danilo Nagib Salomão; Musso,Carlos; Boasquevisque,Paulo César RibeiroPURPOSE: To evaluate the effect of decompression fasciotomy on testes undergoing torsion of the spermatic cord. METHODS: Spermatic cord torsion was induced in 40 Wistar testes for six and 12 hours. At the time of distortion, decompression fasciotomy was performed on half of the animals. After 42 days, the testicles were examined both macroscopically and microscopically. A non-parametric Mann- Whitney U- test wa...
Immunoexpression of proliferating cell nuclear antigen (PCNA) in spleen of sple...
Paulo,Marcela Souza Lima; Santos,Filipy Taifson Barbosa dos; Rocha,Polyanna Gonçalves; Silva,Mirella de Barros; Cintra,Luiz CálicePURPOSE: To analyze PCNA immunoexpression on the inferior pole of the spleen of splenectomized rats submitted to hyperbaric oxygenation (HBO). METHODS: Were analyzed fragments of the inferior pole of the spleen of 20 male Wistar rats submitted to splenectomy with preservation of the inferior pole. The rats were divided in two groups: group A (n=10) without HBO and group B (n=10) submitted to HBO at 2, 5 atmosph...