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Decoding Klebsiella pneumoniae in poultry chain: Unveiling genetic landscape, a...

Mourão, Joana; Magalhães, Mafalda; Ribeiro-Almeida, Marisa; Rebelo, Andreia; Novais, Carla; Peixe, Luísa; Novais, Ângela; Antunes, Patrícia

The rise of antibiotic resistance in the food chain is influenced by the use of antimicrobial agents, such as antibiotics, metals, and biocides, throughout the entire farm-to-fork continuum. Besides, non-clinical reservoirs potentially contribute to the transmission of critical pathogens such as multidrug-resistant (MDR) Klebsiella pneumoniae. However, limited knowledge exists about the population structure and...


Enterococcus spp. from chicken meat collected 20 years apart overcome multiple ...

Rebelo, Andreia; Duarte, Bárbara; Ferreira, Carolina; Mourão, Joana; Ribeiro, Sofia; Freitas, Ana R.; Coque, Teresa M.; Willems, Rob; Corander, Jukka

Poultry meat has been a vehicle of antibiotic resistant bacteria and genes. Yet, the diversity of selective pressures associated with their maintenance in the poultry-production chain remains poorly explored. We evaluated the susceptibility of Enterococcus spp. from chicken meat collected 20 years apart to antibiotics, metals, acidic pH and peracetic acid-PAA. Contemporary chicken-meat samples (n = 53 batches, ...


From Farm to Fork: Persistence of Clinically Relevant Multidrug-Resistant and C...

Mourão, Joana; Ribeiro-Almeida, Marisa; Novais, Carla; Magalhães, Mafalda; Rebelo, Andreia; Ribeiro, Sofia; Peixe, Luísa; Novais, Ângela

Concerns about colistin-resistant bacteria in animal food-environmental-human ecosystems prompted the poultry sector to implement colistin restrictions and explore alternative trace metals/copper feed supplementation. The impact of these strategies on the selection and persistence of colistin-resistant Klebsiella pneumoniae in the whole poultry production chain needs clarification. We assessed colistin-resistan...


Exploring peracetic acid and acidic pH tolerance of antibiotic-resistant non-ty...

Rebelo, Andreia; Duarte, Bárbara; Freitas, Ana R.; Peixe, Luísa; Antunes, Patrícia; Novais, Carla

Acid stress poses a common challenge for bacteria in diverse environments by the presence of inorganic (e.g., mammals’ stomach) or organic acids (e.g., feed additives; acid-based disinfectants). Limited knowledge exists regarding acid-tolerant strains of specific serotypes, clonal lineages, or sources in human/animal pathogens: namely, non-typhoidal Salmonella enterica (NTS) and Enterococcus faecium (Efm). This...


Assessment of antimicrobial resistance spread in surface aquatic systems: a stu...

Matos, Cátia; Duarte, Bárbara; Rebelo, Andreia; Lopes, Diana; Freitas, Ana R.; Valente, Margarida; Tavares, Carolina; Rodrigues, Juliana; Peixe, Luísa

Antimicrobial resistance (AMR) spread in surface water systems, providing water for drinking or for recreational activities, can have serious implications to human, animal and environmental health. The standard criteria to assess water quality are based in the absence of specific bacteria, and do not include AMR study. Here we evaluate the spread of AMR bacteria in rivers of North Portugal under diverse anthrop...


Unraveling the role of metals and organic acids in bacterial antimicrobial resi...

Rebelo, Andreia; Almeida, Agostinho; Peixe, Luísa; Antunes, Patrícia; Novais, Carla

Antimicrobial resistance (AMR) has a significant impact on human, animal, and environmental health, being spread in diverse settings. Antibiotic misuse and overuse in the food chain are widely recognized as primary drivers of antibiotic-resistant bacteria. However, other antimicrobials, such as metals and organic acids, commonly present in agri-food environments (e.g., in feed, biocides, or as long-term polluta...


Uncovering the effects of copper feed supplementation on the selection of coppe...

Rebelo, Andreia; Duarte, Bárbara; Freitas, Ana R.; Almeida, Agostinho; Azevedo, Rui; Pinto, Edgar; Peixe, Luísa; Antunes, Patrícia; Novais, Carla

The use of antibiotics in animal production is linked to the emergence and spread of antibiotic-resistant bacteria, a threat to animal, environmental and human health. Copper (Cu) is an essential element in poultry diets and an alternative to antibiotics, supplementing inorganic or organic trace mineral feeds (ITMF/OTMF). However, its contribution to select multidrug-resistant (MDR) and Cu tolerant Enterococcus...


Enterococcus spp. from chicken meat collected 20 years apart overcome multiple ...

Rebelo, Andreia; Duarte, Bárbara; Ferreira, Carolina; Mourão, Joana; Ribeiro, Sofia; Freitas, Ana R.; Coque, Teresa M.; Willems, Rob; Corander, Jukka

Poultry meat has been a vehicle of antibiotic resistant bacteria and genes. Yet, the diversity of selective pressures associated with their maintenance in the poultry-production chain remains poorly explored. We evaluated the susceptibility of Enterococcus spp. from chicken meat collected 20 years apart to antibiotics, metals, acidic pH and peracetic acid-PAA. Contemporary chicken-meat samples (n = 53 batches, ...


From farm-to-fork: occurrence of persistent multidrug-resistant and copper-tole...

Magalhães, Mafalda; Almeida, Marisa Ribeiro; Mourão, Joana; Rebelo, Andreia; Lopes, Diana; Peixe, Luísa; Novais, Ângela; Novais, Carla

Klebsiella pneumoniae-Kp is a clinically-relevant but neglected multidrugresistant-MDR bacteria at the One Health interface. Studies on poultry production to depict factors contributing to Kp selection/persistence remain scarce. Here we evaluated Kp occurrence and diversity among chicken flocks, its environment and derived meat produced in 7 intensive farms-FA where copper-supplemented feeds are widely used.


Assessment of antimicrobial resistance spread in surface aquatic systems: a stu...

Matos, Cátia; Duarte, Bárbara; Rebelo, Andreia; Lopes, Diana; Freitas, Ana R.; Valente, Margarida.; Tavares, Carolina,.; Rodrigues, Juliana.


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