43 documents found, page 1 of 5
Effective separation of nucleic acids from complex bacterial lysates using thre...
Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, FaniNo abstract available.
The use of thermoreversible ionic liquid-based aqueous biphasic systems as an i...
Silva, Luís C. V.; Sousa, Maria I.; Correia, Andreia; Freire, Mara G.; Silva, Francisca A. e; Pedro, Augusto Q.COVID-19 reinforced the potential of messenger RNA (mRNA) vaccines as effective platforms to control disease outbreaks, given their rapid development, safety and efficacy. Nevertheless, mRNA manufacturing can be optimized by combining production with initial clarification steps. This work aims to integrate mRNA production and initial clarification steps, using thermoreversible ionic liquid-based aqueous biphasi...
Recombinant laccase biosynthesis for efficient polydopamine coating
Magalhães, Flávia F.; Neves, Márcia C.; Pedro, Augusto Q.; Freire, Mara G.; Santos-Ebinuma, Valéria C.; Tavares, Ana P. M.Laccases are versatile biocatalysts with interest for various industrial applications. This study reports the expression of Trametes versicolor laccase in Komagataella phaffii X33. The cultivation parameters (methanol and CuSO4 concentration, and temperature) for recombinant laccase production were studied in an orbital shaker. Enhanced laccase production was achieved by adding 1 % (v/v) methanol daily, supplem...
Purification of RNA from recombinant lysates using biocompatible amino-acid-bas...
Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, FaniRNA-based biopharmaceuticals are being envisioned as a powerful tool for the development of innovative medicines for prevalent diseases. Despite their undeniable relevance, the ubiquitous unstable nature of RNA coupled with the laborious and costly methods required for its extraction and purification still challenge the widespread application of this biopolymer. On the basis of the high affinity between amino-a...
Aqueous biphasic systems composed by biocompatible amino acid-based ionic liqui...
Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, FaniThe emergence of RNA as a promising biopharmaceutical paved the way for the development of innovative medicines with broad therapeutic and prophylactic efficiencies in infectious diseases and cancer. Notwithstanding their relevance on this field, the labile nature and intrinsic low stability of RNA coupled with the laborious and costly methods of extraction and purification of this biopolymer constitute the mai...
Development of an integrated messenger RNA manufacturing process using thermore...
Sousa, Maria I.; Silva, Luís; Freire, Mara G.; Tavares, Ana P. M.; Silva, Francisca A. e; Pedro, Augusto Q.The promising potential of messenger RNA (mRNA) vaccines as effective approaches to contain the dissemination of infectious diseases was fully disclosed during the combat to the COVID-19 pandemic. Over conventional vaccines, mRNA-based vaccines exhibit improved safety and efficacy profiles, and the possibility of repeatedly administration [1]. However, the manufacturing of mRNA vaccines is complex, costly and r...
Using thermoreversible aqueous biphasic systems and ionic liquids to improve th...
Sousa, Maria I.; Silva, Luís C. V.; Correia, Andreia; Freire, Mara G.; Silva, Francisca A. e; Pedro, Augusto Q.If there is one thing the COVID 19 pandemic has taught us is the enormous potential of messenger RNA (mRNA) vaccines as an effective tool to contain infectious disease outbreaks. Over conventional vaccines, there are several advantages to mRNA vaccines, namely improved safety and efficacy, and the possibility of repeatedly administration [1]. However, mRNA nanomedicine production is still a complex and expensiv...
Improving the manufacturing of mRNA nanomedicines using thermoreversible aqueou...
Sousa, Maria I.; Silva, Luís C. V.; Correia, Andreia; Freire, Mara G.; Silva, Francisca A. e; Pedro, Augusto Q.It is undeniable the potential of messenger RNA (mRNA) vaccines as effective tools to manage infectious disease outbreaks. However, mRNA nanomedicines production is complex and expensive, requiring improved technologies to produce stable and widely accessible products, whilst meeting required production times and fulfilling market demands. Ionic liquids (ILs) are composed of organic cations and organic or inorg...
Optimization of the production and purification of non-viral vectors for gene t...
Valente, Ana I.; Castro, Leonor S.; Rios, Inês D.; Pedro, Augusto Q.; Tavares, Ana P. M.; Sousa, Fani; Freire, Mara G.Cancer ranks the second leading cause of death globally and has significant societal and economic costs. To address this issue, the production of biopharmaceuticals is standing out. Nucleic acids are gaining popularity in preventing infections and as therapeutic agents in gene therapy, aiming to fix the target dysfunction by transfecting eukaryotic cells with gene-based products. Non-viral vectors, including pl...
Synthesis and cytotoxicity evaluation of supported ionic liquids for the purifi...
Valente, João Vasco; Valente, Ana I.; Pedro, Augusto Q.; Freire, Mara G.In an aging society, cancer is the second cause of death worldwide with projections of 28.4 million new cases by the year of 2040. Nucleic acids-based biopharmaceuticals, among which the nonviral vector minicicle DNA (mcDNA), are emerging as groundbreaking therapeutic agents for cancer, primarily because of their enhanced therapeutic efficacy, specificity, and reduced occurrence of side effects. [1] Current mcD...