8 documents found, page 1 of 1

One-step purification of L-asparaginase from cell extracts using carbon xerogels

L-asparaginase (ASNase, EC 3.5.1.1) is an enzyme with wide applications in the pharmaceutical sector and food processing industries. It is mainly used as a biotherapeutic for treating Acute Lymphoblastic Leukemia (ALL) and to reduce acrylamide formation in starchy compounds. Despite its relevance, current purification methods for microbial enzymes involve complex and expensive techniques. To overcome this drawb...

Supported ionic liquids to purify microbial L-Asparaginase

L-Asparaginase (ASNase) is a versatile enzyme that converts L-asparagine into ammonia and aspartic acid. This enzyme has applications in the food industry and health sector. However, high purity ASNase is required, resulting in high production costs. Therefore, in this work, two supported ionic liquids (SILs), specifically silica modified with dimethylbutylpropylammonium chloride ([Si][N3114]Cl) or triethylprop...

Supported ionic liquids for L-asparaginase downstream processing

L-asparaginase is an enzyme applied as a biopharmaceutical, as an acrylamide reduction agent and in the development of biosensors for the detection of L-asparagine (Nunes et al., 2020). L-asparaginase is widely distributed in nature, but microorganisms are the preferential source of production since they easily grow on inexpensive substrates (Lopes et al., 2017). For all the described applications, high levels ...

Purification of L-asparaginase through supported ionic liquid

L-asparaginase (ASNase) is an amidohydrolase enzyme applied as an anticancer agent in lymphoproliferative disorders treatment, as an acrylamide reduction agent in starch-rich foods cooked at high temperatures, and as a L-asparagine biosensor. ASNase is widely distributed in nature, e.g., microorganisms, plants and tissues of several animals. Nevertheless, most commercial ASNase are from recombinant microorganis...

Novel flow-through like mode platform for the purification of L-asparaginase

L-Asparaginase, an amidohydrolase enzyme applied in the pharmaceutical and food industries, is widely distributed in nature. Microorganisms are the preferential source of L-asparaginase production since they easily grow on inexpensive substrates. However, high levels of enzyme purity are required for all industrial applications, highlighting the need of developing cost-effective L-asparaginase purification proc...

A flow-through strategy using supported ionic liquids for L-asparaginase purifi...

L-asparaginase production review: bioprocess design and biochemical characteris...

In the past decades, production of biopharmaceuticals has gained high interest due to its high sensitivity, specificity and lower risk of negative effects to patients. Biopharmaceuticals are mostly therapeutic recombinant proteins produced through biotechnological processes. In this context, L-Asparaginase (L-Asparagine amidohydrolase, L-ASNase (E.C. 3.5.1.1)) is a therapeutic enzyme that has been abundantly st...

Production and characterization of recombinant Aliivibrio fischeri L-Asparagina...

L-Asparaginase has been successfully applied in the treatment of lymphoid malignancies. Some limitations in the use of the commercial preparations of this drug include several side effects that may be correlated to L-Glutaminase activity, as immunosuppressive effects (Castro et al., 2021). The objective of this study was to evaluate the characteristics of a novel engineered Aliivibrio fischeri L-asparaginase ty...