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An Efficient Method to Prepare Barcoded cDNA Libraries from Plant Callus for Lo...

Cordeiro, Daniela; Camelo, Alexandra; Pedrosa, Ana Carolina; Brandão, Inês; Canhoto, Jorge; Espirito Santo, Christophe; Correia, Sandra

Long-read sequencing methods allow a comprehensive analysis of transcriptomes in identifying full-length transcripts. This revolutionary method represents a considerable breakthrough for non-model species since it allows enhanced gene annotation and gene expression studies when compared to former sequencing methods. However, woody plant tissues are challenging to the successful preparation of cDNA libraries, th...

Date: 2023   |   Origin: Estudo Geral - Universidade de Coimbra
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