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Milk Antiviral Proteins and Derived Peptides against Zoonoses

Santos, Isabel; Silva, Mariana; Grácio, Madalena; Pedroso, Laurentina; Lima, Ana

Milk is renowned for its nutritional richness but also serves as a remarkable reservoir of bioactive compounds, particularly milk proteins and their derived peptides. Recent studies have showcased several robust antiviral activities of these proteins, evidencing promising potential within zoonotic viral diseases. While several publications focus on milk’s bioactivities, antiviral peptides remain largely neglect...


Milk’s Bioactive Peptides: Are We Disregarding Nature’s Miracle?

Santos, Maria Isabel Silva; Grácio, Madalena; Pedroso, Laurentina; Lima, Ana Isabel Gusmão

Milk has a great nutritional value duo to its amino acid composition and its fat content. However, what makes milk an excellent product for human consumption it’s the presence of bioactive sequences among their aminoacidic profile. Bioactive peptides derived from milk proteins are well known to retain many biological properties and have therapeutic effects in several health disorders. The main source of these c...


Are Food Producing animals a source of multidrug-resistant E. coli and Salmonel...

Pista, Angela; Silveira, Leonor; Gonçalves, Carlota; Costa, Sara; Carolino, Catarina; Santo, Bianca; Rosa, Rute; Penteado, Margarida; Alves, Margarida

Objectives: To assess the role of food-producing animals as potential transmission vehicles of Salmonella spp. and E. coli to Humans, in order to understand the epidemiology and population structure of these zoonotic agents in Portugal.


Multidrug-resistant Escherichia coli and Salmonella spp. in pigs slaughtered fo...

Pista, Angela; Silveira, Leonor; Costa, Sara; Rosa, Rute; Gonçalves, Carlota; Alves, Margarida; Belas, Adriana; Santos, Isabel; Lima, Ana

Objective: To assess the role of pig reservoir as potential transmission vehicle of Salmonella spp. and E. coli to humans, in order to understand the epidemiology and population structure of these zoonotic agents in Portugal.


One Health Perspectives on Food Safety in Minimally Processed Vegetables and Fr...

Santos, Maria Isabel; Grácio, Madalena; Silva, Mariana Camoesas; Pedroso, Laurentina; Lima, Ana

While food markets and food production chains are experiencing exponential growth, global attention to food safety is steadily increasing. This is particularly crucial for ready-to-eat products such as fresh-cut salads and fruits, as these items are consumed raw without prior heat treatment, making the presence of pathogenic microorganisms quite frequent. Moreover, many studies on foodborne illnesses associated...


Compostos bioativos para a saúde e segurança alimentar

Mota, Joana; Belas, Adriana; Marques, Cátia; Teixeira, Daniela; Agrícola, Ricardo; Resendes, Ana; Prazeres, José Manuel Cardoso de Sousa

Atualmente, o tópico dos compostos bioativos para a segurança alimentar tem sido alvo de grande foco, sobretudo numa época em que a utilização de desinfetantes químicos e de antibióticos é cada vez mais evitada na área agro-alimentar, dentro de um contexto One Health. Deste modo, o estudo de compostos moleculares com capacidade de inibir a presença de bactérias ou fungos patogénicos ou de promover uma população...


Industrial waste whey as a low-cost, efficient and environmentally safe disinfe...

Santos, Maria Isabel S.; Lima, Ana Isabel G.; Pedroso, Laurentina; Sousa, Isabel

Being a fast and reliable way to access healthy products, fresh-cut fruit and vegetables are becoming a rapidly rising sector of the horticultural industry with a concomitant high consumer demand. Fresh-cut processing usually involves sanitizing steps, with chlorine washing being the general choice. Due to the health harming effects of chlorine, there has been an increasing demand for alternative disinfecting a...


Extended cheese whey fermentation produces a novel casein-derived antibacterial...

Santos, Maria Isabel; Lima, Ana; Mota, Joana; Rebelo, Patrícia; Ferreira, Ricardo Boavida; Pedroso, Laurentina; Ferreira, Maria Adélia; Sousa, Isabel

Our previous works produced a whey fermentation methodology that yielded antibacterial activity and potential inhibition of matrix metalloproteases (MMP)-2 and -9. Here, we evaluated if these activities were due to fermentation-produced peptides. Prolonged fermentation was carried out in the presence of our specific lactic acid bacteria (LAB) consortium. LAB fermentation yielded a total of 11 polypeptides, whic...


A novel way for whey: cheese whey fermentation produces an effective and enviro...

Santos, Maria Isabel S.; Fradinho, Patrícia; Martins, Sandro; Lima, Ana Isabel G.; Boavida Ferreira, Ricardo; Pedroso, Laurentina

Cheese whey has been described as an environmental hazard due to its high organic content. Although it has been suggested that whey can be used as food disinfectant, it continues to pose an environmental problem because it still contains a high organic load. Here, we aimed to develop a low-cost, scalable fermentation protocol to produce a disinfectant from dairy waste that has very little organic content and hi...


Preliminary study on the effect of fermented cheese whey on Listeria monocytoge...

Santos, Maria I.S.; Lima, Ana I.; Monteiro, Sara A.V.S.; Ferreira, Ricardo Boavida; Pedroso, Laurentina; Sousa, Isabel; Ferreira, Maria A.S.S.

Cheese whey fermented by an industrial starter consortium of lactic acid bacteria was evaluated for its antibacterial capacity to control a selection of pathogenic bacteria. For their relevance on outbreak reports related to vegetable consumption, this selection included Listeria monocytogenes, serotype 4b, Escherichia coli O157:H7, and Salmonella Goldcoast. Organically grown lettuce was inoculated with an inoc...


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