Enterococcus faecium, a human and animal commensal broadly distributed in the environment, is currently one of the most challenging multidrug-resistant (MDR) healthcare-associated pathogens worldwide. It is often exposed to chlorhexidine (CHX), a broad-spectrum antiseptic, extensively used in healthcare, domestic, and food production settings, and a diffused polluter. However, the impact of gradients of CHX con...
Colistin, a last-resort antibiotic in human medicine, has been banned in European food animal production to mitigate antimicrobial resistance. This study investigates the long-term effects of the colistin ban on the occurrence and genomic features (WGS) of colistin-resistant, mcr-carrying Escherichia coli across intensive rabbit farms (8 farms, similar to 600 animals/farm, fecal and farm environmental samples) ...
We aim to characterise multiple ertapenem-resistant (ERT-R, n = 15) Enterobacteriaceae isolates identified as presumptive carbapenemase producers in a Portuguese hospital in a short period of time (March-July 2010). Antibiotic susceptibility patterns, beta-lactamases, genetic relatedness [pulsed-field gel electrophoresis (PFGE), multi-locus sequence typing (MLST)], plasmid content and major enterobacterial pori...
In 200 sulfonamide-resistant Portuguese Salmonella isolates, 152 sull, 74 sul2, and 14 sul3 genes were detected. Class 1 integrons were always associated with sul genes, including sul3 alone in some isolates. The sul3 gene has been identified in isolates from different sources and serotypes, which also carried a class I integron with aadA and dfrA gene cassettes.
The incidence of Salmonella in 60 samples of poultry products of national origin available for consumers obtained from two local butcher shops and one canteen of the city of Porto and the susceptibility to antimicrobial agents allowed for human or animal therapy were evaluated. The results show that poultry samples are frequently contaminated with Salmonella (60%), belonging to 10 different serotypes. Salmonell...
Acinetobacter baumannii 65FFC, an imipenem-resistant clinical strain, isolated from the urine of a patient at the Coimbra University Hospital, Portugal, in 1998, produced a metallo-beta-lactamase with a calculated pI 9.3. The isolate was highly resistant to penicillins, broad-spectrum cephalosporins, including ceftazidime, ceftriaxone, cefepime, cefpirome, and to aztreonam, but it remained susceptible to ampici...