118 documents found, page 1 of 12

Identification of linezolid-resistant Enterococcus spp. in calves: findings fro...

Background: Antimicrobial resistance (AMR) is a global health threat requiring a One Health approach, as antibiotic-resistant bacteria can spread between animals and humans. Enterococcus spp., particularly E. faecium (Efm) and E. faecalis (Efs), are key AMR indicators due to their role as gut commensals and their potential as reservoirs of resistance genes. They are also opportunistic pathogens that can cause s...

Colonization and diversity of Klebsiella pneumoniae species complex (KpSC) in t...

Decoding Klebsiella pneumoniae in poultry chain: Unveiling genetic landscape, a...

The rise of antibiotic resistance in the food chain is influenced by the use of antimicrobial agents, such as antibiotics, metals, and biocides, throughout the entire farm-to-fork continuum. Besides, non-clinical reservoirs potentially contribute to the transmission of critical pathogens such as multidrug-resistant (MDR) Klebsiella pneumoniae. However, limited knowledge exists about the population structure and...

Bacteriocin dynamics in Enterococcus faecium and Enterococcus lactis: implicati...

Background: Enterococcus faecium-Efm and E.lactis-Elts (former Efm-cladeB) colonize the human gut, with Efm also being a leading hospital-pathogen. Dynamics influencing strain dominance in competitive environments (e.g., infection/colonization) are not fully understood, but bacteriocins may provide competitive advantage to clinical Efm or commensal Elts strains. Objective: We explored bacteriocin content of con...

Unravelling global challenges and innovations in antimicrobial resistance

Antimicrobial resistance (AMR) remains a formidable adversary in global health, contributing significantly to mortality from both nosocomial and community-acquired infections [1-6]. This talk aims to dissect the multifaceted nature of AMR, exploring its roots, ongoing global challenges, and innovative countermeasures being developed to mitigate its impact. Despite decades of awareness, AMR continues to escalate...

A challenge in antibiotic stewardship: detection of vancomycin-variable Enteroc...

Background: Vancomycin-variable-enterococci (VVE) are vanA+ enterococci expressing a vancomycin-susceptible phenotype that can revert to a resistant phenotype (VRE) after vancomycin exposure. Objective: We aimed to screen and characterize VVE in a large collection of Enterococcus faecium (Efm) [1]. Methods: We performed a vanA-PCR screening on an extensive Efm collection (2009-2022), including hospital (n=255) ...

Characterization of vancomycin-resistant Enterococcus faecium causing infection...

Background: Vancomycin-resistant Enterococcus faecium (VREfm) are leading nosocomial pathogens linked to high mortality rates and costs [1,2]. VREfm are also included in the WHO global priority list of antibiotic-resistant bacteria for which new antibiotics are urgently needed [3]. Their epidemiology is puzzling within Europe and VREfm data in Portuguese hospitals are lacking since the 2000s. Objective: We...

Epidemiology and discrimination of clinically relevant Enterobacter cloacae com...

Background: E. cloacae complex species are increasingly implicated in infections caused by multidrug-resistant bacteria, but their epidemiology is scarce due to the limitations of automated methods in accurate species identification (e.g. VITEK2/ MALDI–TOF MS) [1]. FT-IR is a promising quick, simple and low-cost alternative for bacterial discrimination [2]. Objective: We aim to assess the epidemiology of Entero...

Genomic and phenotypic heterogeneity of Gardnerella genus – implications for wo...

Background: Gardnerella species have been associated with bacterial vaginosis but are also found in asymptomatic women [1]. Objective:Characterize Gardnerella urogenital isolates from asymptomatic women (HW) and women diagnosed with overactive bladder (OAB), to understand bacterial factors that may influence health outcomes. Methods: Gardnerella isolates (urine, n=5 HW, n=4 OAB) were subjected to WGS (Illu...

Raw meat-based diet for pets: a neglected source of human exposure to Salmonell...

Background: The pet industry is expanding worldwide, particularly raw meat -based diets (RMBDs). There are concerns regarding the safety of RMBDs, especially their potential to spread clinically relevant antibioticresistant bacteria or zoonotic pathogens. Aim: We aimed to investigate whether dog food, including RMBD, commercially available in Portugal can be a source of Salmonella and/or other Enterobacteriacea...