6 documents found, page 1 of 1
DNA damage independent inhibition of NF-κB transcription by anthracyclines
Pedroso, Dora; Chora, Ângelo Ferreira; Kyriakou, Eleni; Pejanovic, Nadja; Colaço, Henrique; Gozzelino, Raffaella; Barros, André; Willmann, KatharinaAnthracyclines are among the most used and effective anticancer drugs. Their activity has been attributed to DNA double-strand breaks resulting from topoisomerase II poisoning and to eviction of histones from select sites in the genome. Here, we show that the extensively used anthracyclines Doxorubicin, Daunorubicin, and Epirubicin decrease the transcription of nuclear factor kappa B (NF-κB)-dependent gene targ...
Changes in Expression of the CLOCK Gene in Obstructive Sleep Apnea Syndrome Pat...
Moreira, Susana; Rodrigues, Raquel; Barros, André B.; Pejanovic, Nadja; Neves-Costa, Ana; Pedroso, Dora; Pereira, Cláudia; Fernandes, DinaMetabolic syndrome and cardiovascular disease are strongly associated with obstructive sleep apnea syndrome (OSAS), which causes substantial changes to normal circadian physiological functions, including metabolic pathways. Because core clock genes are known to be modulated by sleep/vigilance cycles, we asked whether the expression level of mRNA coding for clock genes is altered in non-treated OSAS patients and...
The ubiquitin ligase HERC3 attenuates NF-κB-dependent transcription independent...
Hochrainer, Karin; Pejanovic, Nadja; Olaseun, Victoria A.; Zhang, Sheng; Iadecola, Costantino; Anrather, JosefActivation of NF-κB-dependent transcription represents an important hallmark of inflammation. While the acute inflammatory response is per se beneficial, it can become deleterious if its spatial and temporal profile is not tightly controlled. Classically, NF-κB activity is limited by cytoplasmic retention of the NF-κB dimer through binding to inhibitory IκB proteins. However, increasing evidence suggests that N...
Anthracyclines induce DNA damage response-mediated protection against severe se...
Figueiredo, Nuno; Chora, Ângelo Ferreira; Raquel, Maria Helena; Pejanovic, Nadja; Pereira, Pedro; Hartleben, Björn; Neves-Costa, Ana; Moita, CatarinaSevere sepsis remains a poorly understood systemic inflammatory condition with high mortality rates and limited therapeutic options in addition to organ support measures. Here we show that the clinically approved group of anthracyclines acts therapeutically at a low dose regimen to confer robust protection against severe sepsis in mice. This salutary effect is strictly dependent on the activation of DNA damage ...
Regulation of Nuclear Factor κB (NF-κB) Transcriptional Activity via p65 Acetyl...
Pejanovic, Nadja; Hochrainer, Karin; Liu, Tao; Aerne, Birgit L.; Soares, Miguel P.; Anrather, JosefThe NF-κB family member p65 is central to inflammation and immunity. The purpose of this study was to identify and characterize evolutionary conserved genes modulating p65 transcriptional activity. Using an RNAi screening approach, we identified chaperonin containing TCP1 subunit η (CCTη) as a regulator of Drosophila NF-κB proteins, Dorsal and Dorsal-related immunity factor (Dif). CCTη was also found to regulat...
Identification of mechanisms controlling the transcriptional activity of nuclea...
Pejanovic, NadjaDissertação para a obtenção do grau de doutor em Biologia pelo Instituto de Tecnologia Química e Biológica. Universidade Nova de Lisboa.; Living in an ever-changing environment, cells have evolved numerous mechanisms that allow their assessment of the environment, their communication with each other and the integration of the information in order to adapt to the environmental changes and to maintain their homeo...