14 documents found, page 1 of 2
Influence of Temperature and Humidity on Sample Preservation prior to the Molec...
Fitas, Margarida; Belo, Ana Teresa; Delgado, Inês; Lucena, Sónia; Leitão, AlexandreThe exponential development of resistance to anthelmintics is increasingly threatening the small ruminant production sector. Current diagnostic methods for gastrointestinal strongylid infections depend on the collection of fresh faeces, maintained at a consistent refrigeration temperature of 4°C, with rapid transport and laboratory processing to prevent egg hatching. Implementing molecular diagnostic techniques...
Salivary Biomarkers in the Diagnosis and Monitoring of Metabolic and Endocrine ...
Tvarijonaviciute, Asta; Lucena, Sónia; Capela e Silva, Fernando; Lamy, ElsaThe present chapter revises the current knowledge about the use of salivary biomarkers for metabolic and endocrine disease diagnosis and monitoring in humans and different animal species. Currently, most information available comes from human medicine and studies performed with experimental animals such as mouse or rat, and less from other veterinary species. Furthermore, since obesity and its-related pathologi...
Comparative proteomic analysis of saliva from dogs with and without obesity-rel...
Lucena, Sónia; Coelho, Ana; Capela e Silva, Fernando; Lamy, Elsa; Tvarijonaviciute, AstaDogs develop only some of the components of the human metabolic syndrome (MetS). Thus, in order to study possible MetS-related alterations in dogs, human MetS criteria were adapted to define canine MetS or so-called obesity-related metabolic dysfunction (ORMD). The main objective of this study was to identify changes in the salivary proteome of obese dogs with ORMD in comparison with obese dogs without ORMD whi...
TEACHING OUTSIDE THE CLASSROOM IN SANITARY INSPECTION CURRICULUM - AN OVERALL V...
Lucena, Sónia; Potes, Maria EduardaThe main objective of this work is to share and explain how teaching outside the classroom has been gradually implemented in the fish and fishery contents on the curricular units of Sanitary Inspection of the Master’s in Veterinary Medicine at the Universidade de Évora (Portugal). The implemented strategy comprises the collaboration of veterinary services with fish industry stakeholders for demonstration and tr...
Obesidade Humana e Canina: Factores de Risco
Lucena, Sónia; Lavrador, Catarina; Infante, Paulo; Capela e Silva, Fernando; Tvarijonaviciute, Asta; Lamy, ElsaA prevalência da obesidade humana triplicou em muitos países da União Europeia desde os anos 80, permanecendo o seu aumento a um ritmo alarmante [1]. Nos cães de companhia observou-se, igualmente, um aumento da sua prevalência [1]. Apesar destas evidências, a situação em Portugal não está caracterizada em animais de companhia, e a relação entre as obesidades canina e humana não é conhecida para a população port...
Comparison of protein precipitation methods for two-dimensional electrophoresis...
Lucena, Sónia; Carreira, Maria C.; Rodrigues, Lénia; Capela e Silva, Fernando; Tvarijonaviciute, Asta; Lamy, ElsaDespite saliva being one fluid with growing interest as a source of biomarkers, both in humans and animal models, few studies have been reported that use proteomic approaches for canine saliva analyses. Two-dimensional electrophoresis (2-DE) is considerably used in biomarkers research and their use for dog saliva study may had relevant knowledge about pathology/physiology. The quality of the results obtained us...
Human and canine prevalence obesity and feending habits – a one health approach...
Lucena, Sónia; Lamy, Elsa; Capela, Fernando; Lavrador, Catarina; Tvarijonaviciute, Astabesity has been considered a major issue in many countries of the European region since the 1980s, and obesity prevalence in humans continues to rise at alarming rates. Due to high number of pets in households, obesity should also be considered in the context of the One Health approach, since in several studies a positive relationship between owner and pet obesity has been reported due to their shared lifestyle...
Comparision of protein precipitation methods for two-dimensional electrophoresi...
Lucena, Sónia; Carreira, Maria; Rodrigues, Lénia; Capela e Silva, Fernando; Tvarijonaviciute, Asta; Lamy, ElsaDespite saliva being one fluid with growing interest as a source of biomarkers, both in humans and animal models, few studies have been reported that use proteomic approaches for canine saliva analyses. Two-dimensional electrophoresis (2-DE) is considerably used in biomarker research and its use for dog saliva study may had relevant knowledge about pathology/physiology. The quality of the results obtained using...
The Effect of Breed, Gender, and Acid Stimulation in Dog Saliva Proteome
Lucena, Sónia; Coelho, Ana; Capela e Silva, Fernando; Tvarijonaviciute, Asta; Lamy, ElsaSaliva gained interest as a potential noninvasive source of biomarkers in humans and that interest starts to be extended also to other animal species. For this purpose, the knowledge of the salivary proteome in healthy conditions and the factors that affect it and how they affect it are necessary. The aimof the present study was to assess the effect that gender and breed have in saliva proteome and the changes ...
Identification of changes in salivary proteome in canine obesity-related metabo...
Lucena, Sónia; Lamy, Elsa; Capela e Silva, Fernando; Tvarijonaviciute, Asta; Varela Coelho, AnaThe data observed in present study suggest that canine ORMD is associated with changes in salivary proteome that could reflect altered metabolism in these dogs.