12 documents found, page 1 of 2
Enalapril in combination with benznidazole reduces cardiac inflammation and cre...
Penitente, Arlete Rita; Leite, Ana Luísa Junqueira; Costa, Guilherme de Paula; Shrestha, Deena; Horta, Aline Luciano; Natali, Antônio J.The protozoan Trypanosoma cruzi triggers an inflammatory process in mammalian heart causing events such as fibrosis, changes in the architecture and functionality in this organ. Enalapril, an angiotensin II-converting enzyme inhibitor, is a drug prescribed to ameliorate this heart dysfunction, and appears to exert a potential role in immune system regulation. Our aim was to evaluate the chronic cardiac inflamma...
Use of fluorescence in a modified disector method to estimate the number of myo...
Novaes, Rômulo Dias; Penitente, Arlete Rita; Talvani, André; Natali, Antônio José; Neves, Clóvis Andrade; Maldonado, Izabel Regina Santos CostaConventional disector methods currently require considerable financial, technical and operational costs to estimate the number of cells, including cardyomyocytes, in a 3D area. To use fluorescence microscopy in a modified disector method to determine the number of myocytes in cardiac tissue in normal and pathological conditions. The study employed four-month-old male Wistar rats with weight of 366.25 ± 88.21g r...
Swimming training attenuates contractile dysfunction in diabetic rat cardiomyoc...
Silva, Márcia Ferreira da; Pelúzio, Maria do Carmo Gouveia; Amorim, Paulo Roberto dos Santos; Lavorato, Vitor Neiva; Santos, Natália Pereira doExperimental diabetes promotes contractile dysfunction in cardiomyocytes, but the effects of swimming in this disorder are not known. To test the effects of a swimming training program (STP) on cardiomyocyte contractile dysfunction in rats with experimental diabetes. Wistar rats (age: 30 days; mean body weight: 84.19 g) with diabetes induced by streptozotocin (60 mg/kg body weight; glucose > 300 mg/dl) were div...
Parasite control and skeletal myositis in Trypanosoma cruzi-infected and exerci...
Novaes, Rômulo Dias; Gonçalves, Reggiani Vilela; Penitente, Arlete Rita; Cupertino, Marli do Carmo; Maldonado, Izabel Regina dos Santos CostaNon-pharmacological strategies have been rarely described in the treatment of infectious diseases.Although exercise training has been recently incorporated in the clinical management of Chagas disease,the rationale basis that supports this indication is poorly understood. Thus, we investigated the effectof an aerobic exercise on the parasitism, inflammation and oxidative tissue damage in a murine modelof Trypan...
Uso de fluorescência em um método de disector modificado para estimar o número ...
Novaes, Rômulo Dias; Penitente, Arlete Rita; Silva, André Talvani Pedrosa da; Natali, Antônio José; Neves, Clóvis AndradeFundamento: Métodos convencionais de disector atualmente requerem consideráveis custos financeiros, técnicos e operacionais para estimar o número de células, incluindo cardiomiócitos, em uma área de 3D. Objetivo: Usar a microscopia de fluorescência em um método de disector modificado para determinar o número de miócitos no tecido cardíaco em condições normais e patológicas. Métodos: O estudo empregou ratos Wist...
Modulation of inflammatory and oxidative status by exercise attenuates cardiac ...
Novaes, Rômulo Dias; Gonçalves, Reggiani Vilela; Penitente, Arlete Rita; Bozi, Luiz Henrique Marchesi; Neves, Clóvis AndradeAims: The rational basis that explains the benefits of exercise therapy on Chagas cardiomyopathy (ChC) is poorly understood. This study investigated the impact of an exercise programon exercise performance, heart parasitism, immunoinflammatory response, fibrogenesis, oxidative damage, and cardiomyocytes contractility in experimental ChC. Main methods: Wistar rats were subjected to a 9-week treadmill running tra...
Efeitos da restrição protéica experimental sobre a morfologia do miocárdio e as...
Penitente, Arlete RitaFundação de Amparo a Pesquisa do Estado de Minas Gerais; Nutrition deficits early in life result in adaptive changes which can permanently modify the structure of an organ or tissue. Despite the fact that cardiac function seem to be altered in rats fed low protein diet, available information about mechanics, morphology and ultrastructure of cardiomyocytes in this model is still limited. The present study invest...
Basal and β-Adrenergic cardiomyocytes contractility dysfunction induced by diet...
Penitente, Arlete Rita; Novaes, Rômulo Dias; Silva, Marcelo Eustáquio; Silva, Márcia Ferreira da; Quintão Júnior, Judson Fonseca; Guatimosim, SilviaBackground: The mechanisms responsible for the cardiac dysfunction associated with dietary protein restriction (PR) are poorly understood. Thus, this study was designed to evaluate the effects of PR on calcium kinetics, basal and β-adrenergic contractility in murine ventricular cardiomyocytes. Methods: After breastfeeding male Fisher rats were distributed into a control group (CG, n = 20) and a protein-restrict...
Trypanosoma cruzi infection induces morphological reorganization of the myocard...
Novaes, Rômulo Dias; Penitente, Arlete Rita; Gonçalves, Reggiani Vilela; Silva, André Talvani Pedrosa da; Peluzio, Maria do Carmo GouveiaBackground: This study investigates morphofunctional adaptations of the heart stroma and parenchyma in rats that are chronically infected with Trypanosoma cruzi. Methods: Four-month-old male Wistar rats were randomized into control (n=14) and infected (n=14) groups. Infected animals were inoculated with T. cruzi Y strain. After 9 weeks, the animals were euthanized, and the right atrium (RA) and left ventricle (...
Trypanosoma cruzi infection alters glucose metabolism at rest and during exerci...
Novaes, Rômulo Dias; Gonçalves, Reggiani Vilela; Penitente, Arlete Rita; Silva, André Talvani Pedrosa da; Neves, Clóvis Andrade; Natali, Antônio JoséThis study investigated the effects of Trypanosoma cruzi infection on pancreatic morphology and glucose metabolism at rest and during exercise. Wistar rats were randomized into control (CG = 10) and infected (IG = 10) groups. The IG animals were inoculated with T. cruzi Y strain (300,000 trypomastigotes/50 g). After 9 weeks, the animals were subjected to glucose (OGTT) and insulin (ITT) tolerance tests and a tr...