6 documents found, page 1 of 1
Development of an enzyme-linked immunosorbent assay for atrazine monitoring in ...
Lima, D. L. D.; Schneider, R. J.; Esteves, V. I.The implementation of the Water Framework Directive (2000/60/EC) requires the establishment of monitoring programs. However, conventional procedures for sample preparation prior to chromatographic analysis are rather expensive and time consuming, being the development of cost-effective and easy tool a necessity. The aim of this work was to develop an enzyme-linked immunosorbent assay (ELISA) able to determine a...
Adsorption behavior of 17 alpha-ethynylestradiol onto soils followed by fluores...
Lima, D. L. D.; Calisto, V.; Esteves, V. I.In this study, a simple and rapid procedure for monitoring adsorption of 17 alpha-ethynylestradiol (EE2) onto soil samples was developed. The used method is based on a multiwavelength fluorescence spectral deconvolution (FSD) where the emission fluorescence spectrum of a sample is considered as a linear combination of emission spectra, named reference spectra. The combination of the reference spectra allows the...
Development of an ELISA procedure to study sorption of atrazine onto a sewage s...
Lima, D. L. D.; Silva, C. P.; Schneider, R. J.; Esteves, V. I.Pesticides may contaminate ground and surface waters and one of the major factors governing this property is soil sorption. Sorption can be assessed by batch equilibrium technique which produces lots of extracts with high dissolved organic carbon concentration in which the pesticide concentration has to be determined. We developed an ELISA procedure to analyse atrazine based on polyclonal antibodies (C193) for ...
Sorption-Desorption Behavior of Atrazine on Soils Subjected to Different Organi...
Lima, D. L. D.; Schneider, R. J.; Scherer, H. W.; Duarte, A. C.; Santos, E. B. H.; Esteves, V. I.Sorption of atrazine on soils subjected to three different organic amendments was measured using a batch equilibrium technique. A higher K(F) value (2.20 kg(-1)(mg L(-1))(-)N) was obtained for soil fertilized with compost, which had a higher organic matter (OM) content. A correlation between the K(Foc) values and the percentage of aromatic carbon in OM was observed. The highest K(Foc) value was obtained for the...
Application of MEKC to the monitoring of atrazine sorption behaviour on soils
Lima, D. L. D.; Erny, G. L.; Esteves, V. I.Measuring interactions between soils and xenobiotics is critical to assess the threat to water resources posed by potentially harmful chemicals. Such studies are done via batch experiments where the chemical of interest is incubated with the soil for a long period of time. However, the analysis of the remaining concentration in the aqueous phase is often troublesome, due to dissolved organic matter that can int...
Using capillary electrophoresis for the determination of organic acids in Port ...
Esteves, V. I.; Lima, S. S. F.; Lima, D. L. D.; Duarte, A CThe simultaneous separation and determination of organic acids in several samples of white and red Port wines was performed by capillary zone electrophoresis using indirectUVdetection with 2,6-pyridinedicarboxylic acid as a background electrolyte buffer. Operational parameters like migration time, temperature, voltage and capillary length were optimized. Sixteen samples of red wine and four samples of white win...