62 documents found, page 1 of 7
Large-scale genomic analysis of global Klebsiella pneumoniae plasmids reveals m...
Spadar, Anton; Perdigão, João; Campino, Susana; Clark, Taane G.Background Klebsiella pneumoniae (Kp) Gram-negative bacteria cause nosocomial infections and rapidly acquire antimicrobial resistance (AMR), which makes it a global threat to human health. It also has a comparatively rare hypervirulent phenotype that can lead to severe disease in otherwise healthy individuals. Unlike classic Kp, canonical hypervirulent strains usually have limited AMR. However, after initial ca...
Insight into pathogenomics and phylogeography of hypervirulent and highly-letha...
Mokrousov, Igor; Vyazovaya, Anna; Shitikov, Egor; Badleeva, Maria; Belopolskaya, Olesya; Bespiatykh, Dmitry; Gerasimova, Alena; Perdigão, JoãoBackground The Mycobacterium tuberculosis Beijing genotype is globally spread lineage with important medical properties that however vary among its subtypes. M. tuberculosis Beijing 14717-15-cluster was recently discovered as both multidrug-resistant, hypervirulent, and highly-lethal strain circulating in the Far Eastern region of Russia. Here, we aimed to analyze its pathogenomic features and phylogeographic p...
Genotyping methods and their contributions to the study of tuberculosis dynamic...
Castillos De Ibrahim Das Neves, Yasmin; Reis, Ana Julia; Xavier Maio, Nathália; Vianna, Júlia; Perdigão, João; Bastos Ramis, IvyIntroduction: Mycobacterium tuberculosis genotyping has impacted evolutionary studies worldwide. Nonetheless, its application and the knowledge generated depend on the genetic marker evaluated and the detection technologies that have evolved over the years. Here we describe the timeline of main genotypic methods related to M. tuberculosis in Latin America and the main findings obtained. Methodology: Systematic ...
Emergence of KPC-3- and OXA-181-producing ST13 and ST17 Klebsiella pneumoniae i...
Elias, Rita; Spadar, Anton; Hendrickx, Antoni P A; Bonnin, Remy A; Dortet, Laurent; Pinto, Margarida; Phelan, Jody E; Portugal, Isabel; Campino, SusanaBackground Carbapenem-resistant Klebsiella pneumoniae (CRKP) strains are of particular concern, especially strains with mobilizable carbapenemase genes such as blaKPC, blaNDM or blaOXA-48, given that carbapenems are usually the last line drugs in the β-lactam class and, resistance to this sub-class is associated with increased mortality and frequently co-occurs with resistance to other antimicrobial classes. Ob...
Global, regional, and national burden of meningitis and its aetiologies, 1990 2...
Wunrow, Han Yong; Bender, Rose G; Vongpradith, Avina; Sirota, Sarah Brooke; Swetschinski, Lucien R; Novotney, Amanda; Gray, Authia P; Perdigão, JoãoBackground Although meningitis is largely preventable, it still causes hundreds of thousands of deaths globally each year. WHO set ambitious goals to reduce meningitis cases by 2030, and assessing trends in the global meningitis burden can help track progress and identify gaps in achieving these goals. Using data from the Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2019, we aimed to assess...
Pluronic® F127 Hydrogel Containing Silver Nanoparticles in Skin Burn Regenerati...
Francisco, Pedro; Neves Amaral, Mariana; Neves, Afonso; Ferreira-Gonçalves, Tânia; Viana, Ana S.; Catarino, José; Faísca, Pedro; Simões, SandraPresently, skin burns are considered one of the main public health problems and lack therapeutic options. In recent years, silver nanoparticles (AgNPs) have been widely studied, playing an increasingly important role in wound healing due to their antibacterial activity. This work is focused on the production and characterization of AgNPs loaded in a Pluronic® F127 hydrogel, as well as assessing its antimicrobia...
Spread of Mycobacterium tuberculosis in Southern Brazilian persons deprived of ...
Busatto, Caroline; Possuelo, Lia Gonçalves; Bierhals, Dienefer; de Oliveira, Carolina Larrosa; de Souza, Mariana Quaresma; Fanfa, DandaraTo evaluate the genetic diversity and clustering rates of M. tuberculosis strains to better understand transmission among persons deprived of liberty (PDL) in Rio Grande do Sul (RS), southern Brazil. This is a cross-sectional study, including strains of M. tuberculosis isolated from PDL, stored at the Central Laboratory of RS, in the period from 2013 to 2018. The molecular characterization was performed using t...
A phylogenomic approach for the analysis of colistin resistance-associated gene...
Elias, Rita; Spadar, Anton; Phelan, Jody; Melo-Cristino, José; Lito, Luís; Pinto, Margarida; Gonçalves, Luísa; Campino, Susana; Clark, Taane G.The emergence of carbapenemase-producing Klebsiella pneumoniae strains has triggered the use of old antibiotics such as colistin. This is driving the emergence of colistin resistance in multidrug-resistant strains that underlie life-threatening infections. This study analyses the mutational diversity of 22 genes associated with colistin resistance in 140 K. pneumoniae clinical isolates integrated in a high-reso...
Synthesis, Characterization and Complex Evaluation of Antibacterial Activity an...
Slavchev, Ivaylo M.; Mitrev, Yavor; Shivachev, Boris; Valcheva, Violeta; Dogonadze, Marine; Solovieva, Natalia; Vyazovaya, Anna; Mokrousov, IgorThe synthesis of 20 arylidenecamphors and 15 pyrimidines with camphane skeleton is described in the current report. A modified method for preparation of sterically hindered 2-aminopyrimidines in two steps was demonstrated. The evaluation of their in vitro activity against Mycobacterium tuberculosis H37Rv showed different MIC values (up to 0.91 μM for ketone 39). Compound 35 demonstrated moderate (8.23 μM), but ...
The burden of bacterial antimicrobial resistance in the WHO European region in ...
Mestrovic, Tomislav; Robles Aguilar, Gisela; Swetschinski, Lucien R; Ikuta, Kevin S; Gray, Authia P; Davis Weaver, Nicole; Han, Chieh; Wool, Eve EBackground Antimicrobial resistance (AMR) represents one of the most crucial threats to public health and modern health care. Previous studies have identified challenges with estimating the magnitude of the problem and its downstream effect on human health and mortality. To our knowledge, this study presents the most comprehensive set of regional and country-level estimates of AMR burden in the WHO European reg...