8 documents found, page 1 of 1

Cellulose test strips modified with virus-like particles: Advancing viral immun...

The COVID-19 pandemic has driven immunity acquisition through infection or vaccination, leading to SARS-CoV-2 antibodies. This study introduces a colorimetric biosensor for COVID-19 immunity screening using Virus-Like Particles (VLPs) with SARS-CoV-2 spike protein motifs on a cellulose carrier. The cellulose was chemically modified with DSS to secure the VLPs, verified via FTIR. Detection utilized the Bradford ...

Optimization of enzymes production by Aspergillus niger in solid state fermenta...

The agri-food industry generates significant amounts of by-products that are often discarded or used in low-value applications. Solid by-products of lignocellulolytic structure have been proved to be suitable substrates in solid-state fermentation (SSF) to produce carbohydrases, that have many industrial applications [1]. In this work, the by-products brewer's spent grain (BSG), rice husk (RH), and vine shoot t...

Identification of novel aptamers targeting cathepsin B-overexpressing prostate ...

Increased levels of cathepsin B (CatB), a cysteine protease, have been associated with different types of tumors, including prostate cancer. Hence, the identification of novel targeting ligands homing CatB may be a promising approach for the development of CatB-targeted therapies. In this work, a methodology called systematic evolution of ligands by EXponential enrichment (SELEX) was used to generate and isolat...

Paper-based biosensors for cancer diagnostics

The implementation of a wide diagnostic campaign to diagnose cancer early could save millions of lives and billions of dollars every year. Unfortunately, cancer diagnosis is extremely complicated and current approaches rely on the use of expensive equipment and specialized personnel, which hamper their deployment in low- and middle-income settings. Here, we analyze the technical challenges that must be overcome...

Development of colorimetric cellulose-based test-strip for the rapid detection ...

Given the pandemic situation, there is an urgent need for an accurate test to monitor antibodies anti-SARS-CoV-2, providing crucial epidemiological and clinical information to monitor the evolution of coronavirus disease in 2019 (COVID-19) and to stratify the immunized and asymptomatic population. Therefore, this paper describes a new cellulose-based test strip for rapid and cost-effective quantitative detectio...

Breast cancer: biomarkers and biosensors

Development of a biosensor for detection of cancer biomarkers

Com o avanço tecnológico na área da oncologia, os biomarcadores existentes em sinergia com as técnicas convencionais de diagnostico têm permitido deteção precoce desta patologia. Ainda assim, quando se debruça sobre o panorama clínico a nível nacional, observa-se que estas técnicas constituem um processo moroso, dispendioso, muitas vezes acarretando elevado desconforto ao paciente. Num aspeto mais global, també...

Selection of a new peptide homing SK-BR-3 breast cancer cells

Breast cancer diagnosis remains a challenge, mostly due to its heterogeneity. This reality translates in delayed treatments, increasing treatment aggressiveness and lower chances of overall survival. The conventional detection techniques, although becoming increasingly sophisticated each year, still lack the ability to provide reliable conclusions without being time consuming, expensive and uncomfortable for th...