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Rice husk, brewer’s spent grain, and vine shoot trimmings as raw materials for ...

Guimarães, Ana Cristina Silva Esperança; Mota, Ana C.; Pereira, Ana Sofia Silva; Fernandes, Margarida; Lopes, Marlene; Belo, Isabel

Solid by-products with lignocellulosic structures are considered appropriate substrates for solid-state fermentation (SSF) to produce enzymes with diverse industrial applications. In this work, brewer’s spent grain (BSG), rice husk (RH), and vine shoot trimmings (VSTs) were employed as substrates in SSF with Aspergillus niger CECT 2088 to produce cellulases, xylanases, and amylases. The addition of 2% (NH4)2SO4...


From crude glycerol and volatile fatty acids to biodiesel and other bioproducts...

Pereira, Ana Sofia Silva; Lopes, Marlene; Belo, Isabel

Biodiesel production using microbial lipids derived from low-cost feedstocks could provide a sustainable alternative for the replacement of conventional fossil fuels. This work studied fed-batch and two-stage batch cultures for microbial lipids production by oleaginous yeast Yarrowia lipolytica NCYC 2904 from crude glycerol (a by-product of the biodiesel industry) and volatile fatty acids (obtained during the a...


Yarrowia lipolytica as a biorefinery platform based on low-cost feedstocks

Pereira, Ana Sofia Silva

Atualmente, o biodiesel com origem nos lípidos microbianos tem atraído muita atenção como alternativa aos combustíveis fósseis. No entanto, os preços elevados dos substratos puros usados na produção de lípidos microbianos limitam a sua aplicação. A levedura oleaginosa Yarrowia lipolytica é considerada interessante para a produção sustentável de lípidos a partir de matérias-primas de baixo custo, uma vez que é c...


High-quality biodiesel production from Yarrowia lipolytica NCYC 2904 bio-oil

Pereira, Ana Sofia Silva; Lopes, Marlene; Belo, Isabel

Due to the global increase in energy demands, the depletion of fossil fuel reserves, and the increase in environmental pollution, upsurges the need to create renewable and environmentally friendly energy. Biodiesel, one of the most attractive alternatives for the replacement of conventional fossil fuels, can be produced from bio-oil accumulated by oleaginous yeast. The use of low-cost feedstocks for bio-oil pro...


Enhancing microbial lipids synthesis for biodiesel production by Y. lipolytica ...

Pereira, Ana Sofia Silva; Belo, Isabel; Lopes, Marlene

Microbial lipids produced by Y. lipolytica have the potential to be used as feedstock for the biodiesel industry, but the high costs of pure substrates used for its production are limiting the potential of this application. Volatile fatty acids (VFAs), obtained in anaerobic fermentation of organic wastes, are inexpensive carbon sources for the cost-effective production of microbial lipids. In this work, two-sta...


Yarrowia lipolytica as a biorefinery platform for effluents and solid wastes va...

Lopes, Marlene; Miranda, Sílvia Cristiana Martins; Costa, Ana Rita; Pereira, Ana Sofia Silva; Belo, Isabel

Due to its physiological and enzymatic features, Yarrowia lipolytica produces several valuable compounds from a wide range of substrates. Appointed by some authors as an industrial workhorse, Y. lipolytica has an extraordinary ability to use unrefined and complex low-cost substrates as carbon and nitrogen sources, aiding to reduce the waste surplus and to produce added-value compounds in a cost-effective way. D...


Bio-oil production for biodiesel industry by Yarrowia lipolytica from volatile ...

Pereira, Ana Sofia Silva; Lopes, Marlene; Miranda, Sílvia Cristiana Martins; Belo, Isabel

Microbial lipids-derived biodiesel is garnering much attention owing to its potential to substitute diesel fuel. In this study, lipid accumulation by Yarrowia lipolytica from volatile fatty acids (VFAs) was studied in a lab-scale stirred tank bioreactor. In batch cultures, Y. lipolytica NCYC 2904 was able to grow in 18 g\textperiodcenteredL1 of VFAs (acetate, propionate, and butyrate), and the addition of a co-...


Medidas de interdição de acesso a recintos desportivos: uma perspetiva de análi...

Pereira, Ana Sofia Silva

O desporto em geral e o desporto de competição em particular, assumem um papel preponderante na sociedade do século XXI. Infelizmente por vezes, o espírito de competição toma proporções excessivas, muito para além do saudável e expetável para o que deveria ser um espetáculo desportivo. Não são raras as vezes em que, se relatam incidentes antes, durante e/ou após um evento desportivo. Esse facto tem vindo a preo...


Factors affecting microbial lipids production by Yarrowia lipolytica strains fr...

Pereira, Ana Sofia Silva; Miranda, Sílvia Cristiana Martins; Lopes, Marlene; Belo, Isabel

Volatile fatty acids (VFAs), which can be generated by acidogenesis of organic wastes, are important building blocks for chemicals production, and are intermediates in many bioprocesses such as microbial lipids production. Important factors affecting the bioconversion of VFAs (acetate, propionate and butyrate) by Yarrowia lipolytica W29 and NCYC 2904 for growth and lipids accumulation were studied. Yarrowia lip...


Candida tropicalis as a promising oleaginous yeast for olive mill wastewater bi...

Dias, Bruna; Lopes, Marlene; Ramôa, Renata; Pereira, Ana Sofia Silva; Belo, Isabel

Olive mill wastewater (OMW), which is generated during olive oil production, has detrimental effects on the environment due to its high organic load and phenolic compounds content. OMW is difficult to biodegrade, but represents a valuable resource of nutrients for microbial growth. In this study, yeast strains were screened for their growth on phenolic compounds usually found in OMW and responsible for antimicr...


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