17 documents found, page 1 of 2
Functional and sequence-based metagenomics to uncover carbohydrate-degrading en...
Pereira, Cátia Sofia Santos; Sousa, Joana; Costa, Ângela Maria Araújo; Santos, A. O.; Rito, Teresa; Soares, Pedro; Franco-Duarte, RicardoThe online version contains supplementary material available at: https://doi.org/10.1007/s00253-023-12627-9.; The renewable, abundant, and low-cost nature of lignocellulosic biomass can play an important role in the sustainable production of bioenergy and several added-value bioproducts, thus providing alternative solutions to counteract the global energetic and industrial demands. The efficient conversion of l...
Cymoxanil inhibits respiration through inhibition of mitochondrial complex IV
Mendes, Filipa; Vieira, Tatiana F.; Pereira, Cátia Sofia Santos; Pinto, Mélanie Martins; Castro, Bruno B.; Sousa, Sérgio F.; Sousa, Maria JoãoCymoxanil is a synthetic acetamide fungicide, used against oomycetes. It was first introduced in 1977 and can be used against downy mildew diseases induced by Plasmopara viticola in grapevine cultures and late blight diseases caused by Phytophthora infestans, in tomatoes and potatoes cultures. This fungicide is used in mixed formulations and its higher solubility enables a relatively widespread occurrence in to...
Bovine lactoferrin-loaded plasmonic magnetoliposomes for antifungal therapeutic...
Pereira, Mélanie; Rodrigues, Ana Rita Oliveira; Amaral, Leslie; Côrte-Real, Manuela; Pereira, Cátia Sofia Santos; Castanheira, Elisabete M. S.Bovine lactoferrin (bLf) is a milk-derived protein that exhibits potent broad-spectrum antifungal activity against multiple fungi. bLf is susceptible to degradation, while some of its properties depend on the tertiary structure. So, the encapsulation of bLf in stimuli-responsive therapeutic formulations provides an added value to enhance its biological activities. Plasmonic magnetoliposomes (PMLs) arise as prom...
Exosomes modified with anti-MEK1 siRNA lead to an effective silencing of triple...
Ferreira, Débora Carina Gonçalves Abreu; Pereira, Cátia Sofia Santos; Costa, Marta; Afonso, Julieta Alexandra Pereira; Yang, Sujuan; Hensel, JanineAvailable online 28 September 2023; Triple negative breast cancer (TNBC) is a highly heterogenous disease not sensitive to endocrine or HER2 therapy and standardized treatment regimens are still missing. Therefore, development of novel TNBC treatment approaches is of utmost relevance. Herein, the potential of MAPK/ERK downregulation by RNAi-based therapeutics in a panel of mesenchymal stem-like TNBC cell lines ...
Plasmalemmal V-ATPase as a potential biomarker for lactoferrin-based anticancer...
Pereira, Cátia Sofia Santos; Rodrigues, L. R.; Côrte-Real, ManuelaLactoferrin (Lf) is a milk-derived protein with well-recognized potential as a therapeutic agent against a wide variety of cancers. This natural protein exhibits health-promoting effects and has several interesting features, including its selectivity towards cancer cells, good tolerability in humans, worldwide availability, and holding a generally recognized as safe (GRAS) status. To prompt the rational clinica...
Saccharomyces cerevisiae cells lacking the zinc vacuolar transporter Zrt3 displ...
Terra-Matos, Joana; Teixeira, Marta Oliveira; Pereira, Cátia Sofia Santos; Noronha, Henrique; Domingues, Lucília; Sieiro, Carmen; Gerós, H.Yeast-based bioethanol production from lignocellulosic hydrolysates (LH) is an attractive and sustainable alternative for biofuel production. However, the presence of acetic acid (AA) in LH is still a major problem. Indeed, above certain concentrations, AA inhibits yeast fermentation and triggers a regulated cell death (RCD) process mediated by the mitochondria and vacuole. Understanding the mechanisms involved...
Bridging the gap between lactoferrin and V-ATPase through a multi-stage computa...
Pereira, Cátia Sofia Santos; Rocha, Juliana F.; Fernandes, Henrique S.; Rodrigues, Lígia R.; Côrte-Real, Manuela; Sousa, Sérgio F.Lactoferrin (Lf), a bioactive milk protein, exhibits strong anticancer and antifungal activities [1,2]. The search for Lf targets and mechanisms of action is of utmost importance to enhance its effective applications. A common feature among Lf-treated cancer and fungal cells is the inhibition of a proton pump essential for pH homeostasis called V-ATPase. Lf-driven V-ATPase inhibition leads to cytosolic acidific...
Lactoferrin perturbs intracellular trafficking, disrupts cholesterol-rich lipid...
Pereira, Cátia Sofia Santos; Guedes, Joana Catarina Pereira; Ferreira, Débora Carina Gonçalves Abreu; Rodrigues, L. R.; Côrte-Real, ManuelaAvailable online 16 September 2022; The milk-derived bovine lactoferrin (bLf) is an iron-binding glycoprotein with remarkable selective anticancer activity towards highly metastatic cancer cells displaying the proton pump V-ATPase at the plasma membrane. As studies aiming to dissect the bLf mechanisms of action are critical to improve its efficacy and boost its targeted clinical use, herein we sought to further...
A review on lactoferrin as a proton pump inhibitor
Pereira, Cátia Sofia Santos; Andrés, María T.; Fierro, José F.; Rodrigues, L. R.; Côrte-Real, ManuelaLactoferrin (Lf) is a versatile natural milk-derived protein that exhibits multiple interesting biological activities. Since it is safe for human administration and currently manufactured using low cost and well-established large-scale processes, the Lf scientific community has been devoted at dissecting its mechanisms of action towards its more rational and efficient use for various applications. Emerging lite...
Novel insights on the interaction of lactoferrin with proton pumping ATPases to...
Pereira, Cátia Sofia SantosTese de doutoramento em Biologia (especialidade em Biologia Celular e Saúde); Lactoferrin (Lf) is a naturally occurring iron-binding protein and one of the most well-known milk bioactive compounds. In addition to milk, it is also found in different fluids of mammals including several mucosal surfaces. A multitude of biological activities, such as antimicrobial, antiviral, immunomodulatory and anticancer, have b...