8 documents found, page 1 of 1
Inflammatory and senescence-related effects of polyethylene microspheres on der...
Morgado, Carolina; Silva, Ana; Cruz, Maria Teresa; Pereira, Cláudia F.; Resende, RosaBackground: Microplastics (MPs) have been raising environmental and human health concerns [1]. Polyethylene (PE) is a synthetic organic polymer and is one of the main constituents of plastics [2]. PE MPs are widely used in cosmetics and personal care products due to their cost-effectiveness, versatility and durability [3]. However, their effect on skin cells remains unclear. Exposure of the dermis to these part...
A Perspective on the Link between Mitochondria-Associated Membranes (MAMs) and ...
Fernandes, Tânia; Domingues, M. Rosário M.; Moreira, Paula I.; Pereira, Cláudia F.Mitochondria interact with the endoplasmic reticulum (ER) through contacts called mitochondria-associated membranes (MAMs), which control several processes, such as the ER stress response, mitochondrial and ER dynamics, inflammation, apoptosis, and autophagy. MAMs represent an important platform for transport of non-vesicular phospholipids and cholesterol. Therefore, this region is highly enriched in proteins i...
Mitochondrial Alterations in Fibroblasts of Early Stage Bipolar Disorder Patients
Marques, Ana P.; Resende, Rosa; Silva, Diana F.; Batista, Mariana; Pereira, Daniela; Wildenberg, Brigite; Morais, Sofia; Macedo, António; Pais, ClaudiaThis study aims to evaluate whether mitochondrial changes occur in the early stages of bipolar disorder (BD). Using fibroblasts derived from BD patients and matched controls, the levels of proteins involved in mitochondrial biogenesis and dynamics (fission and fusion) were evaluated by Western Blot analysis. Mitochondrial membrane potential (MMP) was studied using the fluorescent probe TMRE. Mitochondrial morph...
Calcium Signaling in Aging and Neurodegenerative Diseases 2019
Cortes, Luísa; Malva, João; Rego, Ana Cristina; Pereira, Cláudia F.The European Calcium Society (ECS) workshop, which is held every 2 years, is a dedicated meeting of scientists interested in the elucidation of the action of calcium binding, calcium signaling and the study of proteins and organelles, such as mitochondria and endoplasmic reticulum, thereby involved, either in health and disease conditions. The 8th edition of the ECS workshop was organized by a group of research...
Characterization and Cytotoxicity Assessment of the Lipophilic Fractions of Dif...
Oliveira, Cátia S. D.; Moreira, Patrícia; Resende, Judite; Cruz, Maria T.; Pereira, Cláudia F.; Silva, Artur M. S.; Santos, Sónia A. O.Acacia dealbata biomass, either from forest exploitation or from the management of invasive species, can be a strategic topic, namely as a source of high-value compounds. In this sense, the present study aimed at the detailed characterization of the lipophilic components of different morphological parts of A. dealbata and the evaluation of their cytotoxicity in cells representative of different mammals' tissues...
The Eighth ECS Workshop on "Calcium Signaling in Aging and Neurodegenerative Di...
Parys, Jan B.; Pereira, Cláudia F.; Villalobos, CarlosThe European Calcium Society (ECS) is very glad to present the realization of a special issue of the International Journal of Molecular Sciences (IJMS) related to the eighth ECS workshop organized this year around the theme of "Calcium Signaling in Aging and Neurodegenerative Diseases" [...].
Activation of the endoplasmic reticulum stress response by the amyloid-beta 1–4...
Fonseca, Ana Catarina R. G.; Ferreiro, Elisabete; Oliveira, Catarina R.; Cardoso, Sandra M.; Pereira, Cláudia F.Neurovascular dysfunction arising from endothelial cell damage is an early pathogenic event that contributes to the neurodegenerative process occurring in Alzheimer's disease (AD). Since the mechanisms underlying endothelial dysfunction are not fully elucidated, this study was aimed to explore the hypothesis that brain endothelial cell death is induced upon the sustained activation of the endoplasmic reticulum ...
Oxidative glutamate toxicity involves mitochondrial dysfunction and perturbatio...
Pereira, Cláudia F.; Oliveira, Catarina Resende deGlutamate toxicity on PC12 cells is mediated by oxidative stress as a consequence of the inhibition of a cystine uptake system with depletion of GSH. In this study we report that glutamate decreases PC12 cell viability, inhibiting the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). This decrease was prevented by the antioxidants vitamin E, idebenone and -deprenyl, which were als...