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Conductive materials change the composition and activity of butyrate-degrading ...

Braga, Cátia Sofia Neves; Costa, João Carlos Sequeira; Duarte, Maria Salomé; Martins, Gilberto; Pereira, Inês A. C.; Pereira, Luciana; Alves, M. M.

Conductive materials (CM) can improve methane production (MP) efficiency in many methanogenic systems. However, several types of CM exist, and there are uncertainties regarding whether they all improve MP efficiency to the same extent and modulate microbial communities in a similar way. To investigate that, different microbial enrichments with and without activated carbon (AC), magnetite (Mag), and zeolites (Ze...


Microbial activity of the inoculum determines the impact of activated carbon, m...

Braga, Cátia Sofia Neves; Martins, Gilberto; Duarte, Maria Salomé Lira; Soares, O. Salomé G. P.; Pereira, M. Fernando R.; Pereira, Inês A. C.

The conversion of organic matter to methane through anaerobic digestion (AD) process can be enhanced by different materials. However, literature reports show inconsistent results on the effect of materials in different AD systems. In this study, we evaluated the influence of the inoculum's activity on methane production (MP) efficiency in the presence of different materials (activated carbon (AC), magnetite (Ma...


Non-conductive silicon-containing materials improve methane production by pure ...

Braga, Cátia Sofia Macedo; Martins, Gilberto; Soares, O. Salomé G. P.; Pereira, M. Fernando R.; Pereira, Inês A. C.; Pereira, Luciana; Alves, M. M.

Conductive materials (CM) enhance methanogenesis, but there is no clear correlation between conductivity and faster methane production (MP) rates. We investigated if MP by pure cultures of methanogens (Methanobacterium formicicum, Methanospirillum hungatei, Methanothrix harundinacea and Methanosarcina barkeri) is affected by CM (activated carbon (AC), magnetite), and other sustainable alternatives (sand and gla...


Insight into the sulfur metabolism of Desulfurella amilsii by differential prot...

Florentino, Anna P.; Pereira, Inês A. C.; Boeren, Sjef; Born, Michael; Stams, Alfons Johannes Maria; Sánchez-Andrea, Irene

Many questions regarding proteins involved in microbial sulfur metabolism remain unsolved. For sulfur respiration at low pH, the terminal electron acceptor is still unclear. Desulfurella amilsii is a sulfur-reducing bacterium that respires elemental sulfur (S0) or thiosulfate, and grows by S0 disproportionation. Due to its versatility, comparative studies on D. amilsii may shed light on microbial sulfur metabol...


An electrogenic redox loop in sulfate reductionreveals a likely widespread mech...

G. Duarte, Américo; Catarino, Teresa; White, Gaye F.; Lousa, Diana; Neukirchen, Sinje; Soares, Cláudio; Sousa, Filipa L; Clarke, Thomas A.

The bioenergetics of anaerobic metabolism frequently relies on redox loops performed by membrane complexes with substrate- and quinone-binding sites on opposite sides of the membrane. However, in sulfate respiration (a key process in the biogeochemical sulfur cycle), the substrate- and quinone-binding sites of the QrcABCD complex are periplasmic, and their role in energy conservation has not been elucidated. He...


Genome analysis of Desulfotomaculum gibsoniae strain GrollT a highly versatile ...

Kuever, Jan; Visser, Michael; Loeffler, Claudia; Boll, Mathias; Worm, Petra; Sousa, D. Z.; Plugge, Caroline M.; Schaap, Peter J.; Muyzer, Gerard

Desulfotomaculum gibsoniae is a mesophilic member of the polyphyletic spore-forming genus Desulfotomaculum within the family Peptococcaceae. This bacterium was isolated from a freshwater ditch and is of interest because it can grow with a large variety of organic substrates, in particular several aromatic compounds, short-chain and medium-chain fatty acids, which are degraded completely to carbon dioxide couple...


Genome analyses of the carboxydotrophic sulfate-reducers Desulfotomaculum nigri...

Visser, Michael; Parshina, Sofiya N.; Alves, Joana I.; Sousa, D. Z.; Pereira, Inês A. C.; Muyzer, Gerard; Kuever, Jan; Lebedinsky, Alexander V.

Desulfotomaculum nigrificans and D. carboxydivorans are moderately thermophilic members of the polyphyletic spore-forming genus Desulfotomaculum in the family Peptococcaceae. They are phylogenetically very closely related and belong to 'subgroup a' of the Desulfotomaculum cluster 1. D. nigrificans and D. carboxydivorans have a similar growth substrate spectrum; they can grow with glucose and fructose as electro...


Genome analysis of Desulfotomaculum kuznetsovii strain 17T reveals a physiologi...

Visser, Michael; Worm, Petra; Muyzer, Gerard; Pereira, Inês A. C.; Schaap, Peter J.; Plugge, Caroline M.; Kuever, Jan; Parshina, Sofiya N.

Desulfotomaculum kuznetsovii is a moderately thermophilic member of the polyphyletic spore-forming genus Desulfotomaculum in the family Peptococcaceae. This species is of interest because it originates from deep subsurface thermal mineral water at a depth of about 3000 m. D. kuznetsovii is a rather versatile bacterium as it can grow with a large variety of organic substrates, including short-chain and long-chai...


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