8 documents found, page 1 of 1
Injectable hydrogel as a carrier of vancomycin and a cathelicidin-derived pepti...
Machado, Fátima Alexandra Meira; Pereira, Isabel Sofia Melo; Silva, Vanessa; Pires, Isabel; Prada, Justina; Poeta, Patrícia; Costa, LuísA local drug delivery system that attempts to find a suitable balance between antimicrobial and regenerative actions was developed for osteomyelitis treatment (OM). This system combines the angiogenic and immunomodulatory peptide LLKKK18 (LL18) and vancomycin hydrochloride (VH), loaded into an injectable oxidized dextrin (ODEX)-based hydrogel (HG). In vitro cytotoxicity was analyzed in MC3T3-E1 pre-osteoblasts ...
Regeneration of critical-sized defects, in a goat model, using a dextrin-based ...
Pereira, Isabel Sofia Melo; Pereira, José Eduardo; Maltez, Luís; Rodrigues, Alexandra; Rodrigues, Catarina; Oliveira, Manuela; Silva, Dina M.The development of injectable bone substitutes (IBS) have obtained great importance in the bone regeneration field, as a strategy to reach hardly accessible defects using minimally invasive techniques and able to fit to irregular topographies. In this scenario, the association of injectable hydrogels and bone graft granules is emerging as a well-established trend. Particularly, in situ forming hydrogels have ar...
In vitro genotoxicity assessment of an oxidized dextrin-based hydrogel for biom...
Pereira, Isabel Sofia Melo; Fraga, Sónia; Silva, Susana; Teixeira, João Paulo; Gama, F. M.Hydrogels are threedimensional, crosslinked networks of hydrophilic polymers swollen with a large amount of water or biological fluids, without dissolving. Dextrin, a lowmolecularweight carbohydrate composed by glucose residues, has been used to develop an injectable hydrogel for biomedical applications. Dextrin was first oxidized to introduce aldehyde groups, which then reticulate with adipic acid dihydrazide,...
Dextrin injectable hydrogel for tissue healing and regeneration
Pereira, Isabel Sofia MeloThe worldwide incidence of bone disorders is increasing, mainly due to ageing population. The lack of effective treatments stimulates the development of novel synthetic bone substitutes (SBSs). Ceramic based-SBSs commercially available display poor handling properties. Attempting to overcome this shortcoming improving the acceptance of these formulations, granular ceramics have been associated with hydrogels. H...
Effects of gamma irradiation and periodate oxidation on the structure of dextri...
Pereira, Isabel Sofia Melo; Simões, Joana; Evtyugin, Dmitry V.; Rouif, Sophie; Coimbra, Manuel A.; Domingues, M. Rosário M.; Gama, F. M.Sterilization of biomaterials by gamma irradiation should not change its structure, since this may affect also its bioactivity. In this work, dextrin and periodate-oxidized dextrin (ODEX), both irradiated and non-irradiated, were hydrolysed by -amylase and the obtained oligosaccharides were fractionated by ligand-exchange/size exclusion chromatography and analyzed by matrix-assisted laser desorption/ionization ...
Formulações de hidrogel de dextrino para a regeneração de defeitos ósseos e não...
Gama, F. M.; Pereira, Isabel Sofia Melo; Rodrigues, A.; Rodrigues, A.; Oliveira, ManuelaA presente invenção consiste em formulações, à base de hidrogel de dextrino, que servem de matriz de suporte e veículo de substituto ósseos granulares para regeneração óssea. especificamente, esta invenção consiste em hidrogéis injetáveis e biorreabsorvíveis, de preparação in situ, os quais atuam como um suporte tridimensional (scaffold) para a colonização celular. a referida formulação contem granulados bioati...
Structural analysis of dextrins and characterization of dextrin-based biomedica...
Silva, Dina M.; Nunes, Cláudia; Pereira, Isabel Sofia Melo; Moreira, Ana S. P.; Domingues, M. R. M.; Coimbra, Manuel A.; Gama, F. M.The characterization of several commercial dextrins and the analysis of the potential of dextrin derived hydrogels for biomedical applications were performed in this work. The structural characterization of dextrins allowed the determination of the polymerization and branching degrees, which ranged from 6 to 17 glucose residues and 2 to 13%, respectively. Tackidex, a medical grade dextrin was choosen for furthe...
Estudos preliminares da bioatividade de hidrolisados de matriz extracelular
Pereira, Isabel Sofia MeloA medicina regenerativa e a engenharia de tecidos têm como objetivo promover a regeneração de tecidos ou substituir os órgãos não funcionais, através da combinação de um scaffold, células adequadas e moléculas bioativas. Os scaffolds de matriz extracelular descelularizada (MEC) têm sido utilizados com sucesso em aplicações da medicina regenerativa para facilitar e promover a remodelação construtiva de inúmeros ...