6 documents found, page 1 of 1
Fighting fungal infections: development of a new diagnosis method and a novel a...
Pereira, Joana Isabel CarvalhoA incidência das infeções fúngicas invasivas (IFI) tem aumentado significativamente devido ao desenvolvimento de intervenções médicas cada vez mais agressivas, aumentando o número de indivíduos imunocomprometidos. Candida spp. e Aspergillus spp. são os organismos mais frequentemente presentes, contudo, nos últimos anos a incidência de Rhizopus spp. tem aumentado. A eficácia do tratamento das IFI depende da susc...
Multiplex PCR system for fungal pathogen detection
Pereira, Joana Isabel Carvalho; Springer, J.; Buítrago, M. J.; Löffler, J.; Pais, Célia; Sampaio, P.Introduction: The rapid and correct identification of pathogenic species is crucial for several reasons that differ according to the area of interest. In clinical laboratories it is important since knowing the pathogen guides appropriate treatment, dose and duration of therapy. In clinical microbiology laboratories culture and microscopic examination remain the “gold standard”. Biochemical and mass spectometry ...
High variability within Candida albicans transcription factor RLM1: Isolates fr...
Pereira, Joana Isabel Carvalho; Oliveira, Manoel M. Evangelista; Barbosa, Augusto Alexandre Costa; Vaz, C.; Velegraki, A.; Zancopé-Oliveira, R. M.Previous studies have correlated the severity of recurrent vulvovaginal Candida infections (VVC) and balanitis in patients from China with the presence of some dominant genotypes at the ORF RLM1. Here we tested VVC vs non-VVC isolates from Portugal, Brazil and Greece and, although the same genotypes were identified in VVC isolates, they were present in only five out of 150 strains. However, this analysis showed...
Genetic variability of Candida albicans SAP8 propeptide in isolates from differ...
Pereira, Joana Isabel Carvalho; Vaz, Catarina Oliveira; Carneiro, Maria Catarina Santos; Pais, Célia; Sampaio, PaulaThe secreted aspartic proteases (Saps) are among the most studied virulence determinants in Candida albicans. These proteins are translated as pre-pro-enzymes consisting of a signal sequence followed by a propeptide and the mature enzyme. The propeptides of secreted proteinases are important for the correct processing, folding/secretion of the mature enzyme. In this study, the DNA sequences of C. albicans Saps ...
A new method for yeast phagocytosis analysis by flow cytometry
Carneiro, Maria Catarina Santos; Vaz, Catarina Oliveira; Pais, Célia; Sampaio, Paula; Pereira, Joana Isabel CarvalhoHerein we developed a method based on the quenching effect of propidium iodide over Sytox-Green fluorescence to assess yeast phagocytosis by flow cytometry. It allows accurate quantification of living from dead phagocytes; internalized from non-internalized cells, maintaining yeast fluorescence within phagocytes; and the different associations between phagocytes and fungal cells. (C) 2014 Elsevier B.V. All righ...
Development of biomarkers for the identification of pathogenic Candida species
Pereira, Joana Isabel CarvalhoIn the last decades the incidence of fungal infections has increased exponentially, due to the development of more aggressive therapeutic techniques, which increase the number of immunocompromised and in risk individuals. The Candida species are the most common etiological agents isolated from opportunistic fungal infections in these patients and Candida albicans appears to be the most common species isolated. ...