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Genomic Analysis of Antibiotic Resistance and Virulence Profiles in Escherichia...

Ribeiro, Jessica; Silva, Vanessa; Freitas, Catarina; Pinto, Pedro; Vieira-Pinto, Madalena; Batista, Rita; Nunes, Alexandra; Gomes, João Paulo

Sternal bursitis is an underexplored lesion in poultry, often overlooked in microbiological diagnostics. In this study, we characterized 36 Escherichia coli isolates recovered from sternal bursitis in broiler chickens, combining phenotypic antimicrobial susceptibility testing, PCR-based screening, and whole genome sequencing (WGS). The genetic analysis revealed a diverse population spanning 15 sequence types, i...

Date: 2025   |   Origin: Biblioteca Digital do IPB

A Review of Novel Heat Transfer Materials and Fluids for Aerospace Applications

Nobrega, Glauco Tapijara Vallicelli; Cardoso, Beatriz D.; Souza, Reinaldo Rodrigues de; Pereira, José Eduardo; Pontes, Pedro; Catarino, Susana O.

The issue of thermal control for space missions has been critical since the early space missions in the late 1950s. The demands in such environments are heightened, characterized by significant temperature variations and the need to manage substantial densities of heat. The current work offers a comprehensive survey of the innovative materials and thermal fluids employed in the aerospace technological area. In ...

Date: 2024   |   Origin: Biblioteca Digital do IPB

Experimental evaluation of green nanofluids in heat exchanger made oF PDMS

Nobrega, Glauco Tapijara Vallicelli; Souza, Reinaldo Rodrigues de; Cardoso, Beatriz D.; Afonso, Inês Santos; Pereira, José Eduardo; Cardoso, Elaine

Conventional methods for synthesizing metallic nanoparticles face challenges such as instability and environmental concerns. Therefore, new, simpler, and more eco-friendly methods are being explored. In this context, the study reports a green synthesis process to produce magnetic iron oxide nanoparticles using an aqueous extract of the alga Chlorella vulgaris. This process leverages natural resources to create ...

Date: 2024   |   Origin: Biblioteca Digital do IPB

Unveiling Antibiotic Resistance, Clonal Diversity, and Biofilm Formation in Iso...

Silva, Adriana; Silva, Vanessa; Dapkevicius, Maria de Lurdes Enes; Azevedo, Mónica; Cordeiro, Rui; Pereira, José Eduardo; Valentão, Patrícia

Escherichia coli, a commensal microorganism found in the gastrointestinal tract of human and animal hosts, plays a central role in agriculture and public health. Global demand for animal products has promoted increased pig farming, leading to growing concerns about the prevalence of antibiotic-resistant E. coli strains in swine populations. It should be noted that a significant portion of antibiotics deployed i...


Randomized clinical study of injectable dextrin-based hydrogel as a carrier of ...

Machado, Alexandra; Pereira, Isabel; Costa, Filomena; Brandão, Ana; Pereira, José Eduardo; Maurício, Ana Colette; Santos, José Domingos; Amaro, Inês

Objectives This study aimed to improve the performance and mode of administration of a glass-reinforced hydroxyapatite synthetic bone substitute, Bonelike by Biosckin® (BL®), by association with a dextrin-based hydrogel, DEXGEL, to achieve an injectable and moldable device named DEXGEL Bone. Methods Twelve participants requiring pre-molar tooth extraction and implant placement were enrolled in this study. BL® g...


Dextrin hydrogel loaded with a macroporous Bonelike® scaffold and dental pulp s...

Machado, A.; Pereira, Isabel; Pereira, José Eduardo; Maltez, Luís; Brandão, Ana; Alvites, Rui; Sousa, Ana Catarina; Branquinho, Mariana

Regeneration of severe bone defects remains a challenge. A formulation of synthetic glass-reinforced hydroxyapatite bone substitute, Bonelike® Poro (BL®P), 250500 µm-diameter, with a dextrin-based hydrogel (HG), further loaded with human dental pulp stem cells (hDPSCs) with osteogenic differentiation ability, was tested for the management of critical-sized defects in an ovine model. Morphology, calcium release,...


Randomized clinical study of injectable dextrin-based hydrogel as a carrier of ...

Machado, Alexandra; Pereira, Isabel; Costa, Filomena; Brandão, Ana; Pereira, José Eduardo; Maurício, Ana Colette; Santos, José Domingos; Amaro, Inês

This study aimed to improve the performance and mode of administration of a glass-reinforced hydroxyapatite synthetic bone substitute, Bonelike by Biosckin® (BL®), by association with a dextrin-based hydrogel, DEXGEL, to achieve an injectable and moldable device named DEXGEL Bone.


Dextrin hydrogel loaded with a macroporous Bonelike® scaffold and dental pulp s...

Machado, Alexandra; Pereira, Isabel; Pereira, José Eduardo; Maltez, Luís; Brandão, Ana; Alvites, Rui; Sousa, Ana Catarina; Branquinho, Mariana

Regeneration of severe bone defects remains a challenge. A formulation of synthetic glass-reinforced hydroxyapatite bone substitute, Bonelike® Poro (BL®P), 250–500 µm-diameter, with a dextrin-based hydrogel (HG), further loaded with human dental pulp stem cells (hDPSCs) with osteogenic differentiation ability, was tested for the management of critical-sized defects in an ovine model. Morphology, calcium release...


Multidrug-Resistant Methicillin-Resistant Coagulase-Negative Staphylococci in H...

Silva, Vanessa; Caniça, Manuela; Ferreira, Eugénia; Vieira-Pinto, Madalena; Saraiva, Cândido; Pereira, José Eduardo; Capelo, José Luis

Coagulase-negative staphylococci are commensals that are known to be prevalent in most environments, and they are also an important reservoir of antimicrobial-resistant genes. Staphylococcal infections in animal husbandry are a high economic burden. Thus, we aimed to determine the prevalence and species diversity of methicillin-resistant coagulase-negative staphylococci (MRCoNS) in poultry slaughtered for human...


Antimicrobial resistance and clonal lineages of staphylococcus aureus from catt...

Silva, Vanessa; Correia, Susana; Rocha, Jaqueline; Manaia, Célia M.; Silva, Adriana; García-Díez, Juan; Pereira, José Eduardo; Semedo-Lemsaddek, Teresa

Staphylococcus aureus have been progressively identified in farm animals and in humans with direct contact with these animals showing that S. aureus may be a major zoonotic pathogen. Therefore, we aimed to isolate S. aureus from cows, their handlers, and their immediate surroundings, and to investigate the antimicrobial resistance and genetic lineages of the isolates. Mouth and nose swabs of 244 healthy cows (1...


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