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Deep proteome analysis of gerontoplasts from the inner integument of developing...
Shah, Mohibullah; Soares, Emanoella L.; Lima, Magda L. B.; Pinheiro, Camila B.; Soares, Arlete A.; Domont, Gilberto B.; Nogueira, Fabio C. S.The inner integument of Jatropha curcas seeds is a non-photosynthetic tissue that acts primarily as a conduit for the delivery of nutrients to the embryo and endosperm. In this study we performed a histological and transmission electron microscopy analysis of the inner integument in stages prior to fertilization to 25 days after pollination, to establish the structural changes associated with the plastid to ger...
Proteome analysis of secondary somatic embryogenesis in cassava (Manihot escule...
Ibrahim, Abdulrazak Baba; Nogueira, Fabio C. S.; Pinheiro, Camila B.; Brasil, Juliana N.; Jereissati, Emmanuel S.; Jucá, Thiago LustosaUsing histological analysis of the induction of secondary somatic embryogenesis (SSE) in cassava (Manihot esculenta Crantz) as a guide, we performed 2-DE for protein separation and matrix-assisted laser desorption ionization-time of flight-tandem mass spectrometry (MALDI-TOF-TOF-MS) for protein identification in cotyledons of cassava somatic embryos undergoing SSE. Reference map obtained by 2- DE within a pH ra...