Aim To evaluate the synergistic activity of antimicrobial drugs against lineages of methicillin-resistant Staphylococcus aureus (MRSA) carrying SCCmec IV. The biofilm production and related genes were also detected. Methods and Results Forty two MRSA isolates were tested for biofilm production and related genes. Biofilm/biomass susceptibility to gentamicin (G), linezolid (L), rifampicin (R) and vancomycin (V) w...
Today, we are facing a major challenge regarding the development of new strategies and the discovery of new compounds with effective antimicrobial outcomes. The emergence of resistance is a preoccupant health threat and conventional antibiotics are being rendered ineffective [1]. Specifically, biofilm related infections are becoming a serious threat, being highly related to chronic infections but also nosocomia...
The main goal of this work was to examine whether the continuous exposure of single and binary P. aeruginosa and E. coli biofilms to sub-lethal benzalkonium chloride (BC) doses can induce adaptive response of bacteria. Biofilms were formed during 24 h and then put continuously in contact with BC for more 5 days. The six-day-old adapted biofilms were then submitted to BC challenge, characterized and inspected by...
Microorganisms attach readily to surfaces and if they are left undisturbed biofilms may form. Biofilm cells normally display an altered phenotype that is believed to be responsible for the less susceptibility to antimicrobials. Moreover, biofilm-growing cells are protected by the self-produced matrix that acts as a shield against external pressures. Thus, pathogens embedded in a biofilm can be harder to remove ...
Croton nepetaefolius is a plant native from northeastern Brazil and belongs to Euphorbiaceae family. The essential oil of this plant is widely used in folk medicine from the treatment of gastrointestinal disorders to the use as an antiseptic agent, with an antifungal action scientifically proven. The action of this plant has been extensively explored by the scientific community, being the secondary metabolites,...
The effectiveness of cetyltrimethyl ammonium bromide (CTAB) and sodium dodecyl sulfate (SDS) to control biofilms formed by Pseudomonas fluorescens on stainless steel slides under laminar and turbulent conditions, using a flow cell reactor, is compared in this study. The antimicrobial action of the surfactants was evaluated in terms of the activity of the biofilm, the biofilm mass that remained on the surface af...