267 documents found, page 1 of 27
Aspartic acid unveils as antibiofilm agent and tobramycin adjuvant against muco...
Monteiro, Rosana Isabel Araújo; Sousa, Ana Margarida; Pereira, Maria OlíviaAntibiotics are central to managing airway infections in cystic fibrosis (CF), yet current treatments often fail due to the presence of Pseudomonas aeruginosa biofilms, settling down the need for seeking therapies targeting biofilms. This study aimed to investigate the antibiofilm activity of aspartic acid and its potential as an adjuvant to tobramycin against P. aeruginosa biofilms formed by mucoid and small c...
Aqueous extracts of Moringa oleifera and Cinnamomum cassia as promising sources...
Silva, Eduarda Daniela Neiva; Santos, Pedro Miguel Ferreira; Teixeira, J. A.; Pereira, Maria Olívia; Rocha, Cristina M.R.; Sousa, Ana MargaridaBacterial biofilms formed by Staphylococcus aureus and Pseudomonas aeruginosa pose significant challenges in treating cystic fibrosis (CF) airway infections due to their resistance to antibiotics. New therapeutic approaches are urgently needed to treat these chronic infections. This study aimed to investigate the antibiofilm potential of various plant extracts, specifically targeting mucoid and small colony var...
Co-immobilization of ciprofloxacin and chlorhexidine as a broad-spectrum antimi...
Alves, Diana Filipa Barros; Pereira, Maria Olívia; Lopes, Susana PatríciaThe endotracheal tube (ETT) affords support for intubated patients, but the increasing incidence of ventilator-associated pneumonia (VAP) is jeopardizing its application. ETT surfaces promote (poly)microbial colonization and biofilm formation, with a heavy burden for VAP. Devising safe, broad-spectrum antimicrobial materials to tackle the ETT bioburden is needful. Herein, we immobilized ciprofloxacin (CIP) and/...
Aptamer-based therapy for fighting biofilm-associated infections
Sousa, Ana Margarida; Ferreira, Débora; Rodrigues, L. R.; Pereira, Maria OlíviaBiofilms are key players in the pathogenesis of most of chronic infections associated with host tissue or fluids and indwelling medical devices. These chronic infections are hard to be treated due to the increased biofilms tolerance towards antibiotics in comparison to planktonic (or free living) cells. Despite the advanced understanding of their formation and physiology, biofilms continue to be a challenge and...
Challenges with drug efficacy prediction of in vitro models of biofilms infecti...
Sousa, Ana Margarida; Pereira, Maria Olívia[Excerpt] Cystic fibrosis (CF) is a genetic disorder caused by a defect in the CF transmembrane conductance regulator gene, characterized by the accumulation of thick, sticky, and acidified sputum in the lungs. This abnormal buildup of sputum, rich in nutrients, and the defective mucociliary clearance render CF patients vulnerable to both acute and chronic bacterial infections of the airways. These infections l...
Biocidal effect of triclosan, glutaraldheyde and CTAB on activated sludge micro...
Ferreira, Vânia Isabel Lopes; Mota, M.; Pereira, Maria Olívia; Nicolau, AnaMixed liquor and real sewage were collected from three wastewater treatment plants, the biocides effect being initially assessed in respirometric assays. The biocide effect was also assessed in bench-scale trials, but, in this case, with mixed liquor and real sewage from only one WWTP. In the respirometric assays, all biocides showed a dose-dependent effect in the inhibition of microbial biomass respiration. In...
Mechanistic insights into succinic acid as an adjuvant for ciprofloxacin in tre...
Monteiro, Rosana Isabel Araújo; Silva, Eduarda; Pereira, Maria Olívia; Sousa, Ana MargaridaPseudomonas aeruginosa is a major cause of chronic respiratory infections in patients with cystic fibrosis (CF), with biofilm formation contributing to its persistence and antibiotic resistance. This study aimed to gain insights into the mechanistic action of succinic acid as a ciprofloxacin adjuvant against clinically relevant CF isolates, including small colony variants and mucoid strains, and a ciprofloxacin...
Selection of specific aptamers for mucoid Pseudomonas aeruginosa biofilms devel...
Carvalho, Maria Beatriz; Rodrigues, L. R.; Pereira, Maria Olívia; Ferreira, Débora; Sousa, Ana MargaridaChronic infections are one of the main contributors for decline of the lung function, respiratory failure and premature death of cystic fibrosis (CF) patients. Pseudomonas aeruginosa is an opportunistic pathogen that commonly infects CF lungs and recognized as the main cause of chronic infections due to its ability of biofilm formation. Being associated with concerning mortality rates, a rapid detection and ide...
Antimicrobial materials for endotracheal tubes: a review on the last two decade...
Alves, Diana Filipa Barros; Graínha, Tânia Raquel Rodrigues; Pereira, Maria Olívia; Lopes, Susana PatríciaVentilator-associated pneumonia (VAP) is an unresolved problem in nosocomial settings, remaining consistently associated with a lack of treatment, high mortality, and prolonged hospital stay in mechanically ventilated patients. The endotracheal tube (ETT) is the major culprit for VAP development owing to its early surface microbial colonization and biofilm formation by multiple pathogens, both critical events f...
Evolving biofilm inhibition and eradication in clinical settings through plant-...
Silva, Eduarda; Teixeira, J. A.; Pereira, Maria Olívia; Rocha, Cristina M. R.; Sousa, Ana MargaridaBackground After almost 100 years since evidence of biofilm mode of growth and decades of intensive investigation about their formation, regulatory pathways and mechanisms of antimicrobial tolerance, nowadays there are still no therapeutic solutions to eradicate bacterial biofilms and their biomedical related issues. Purpose This review intends to provide a comprehensive summary of the recent and most relevant ...