30 documents found, page 1 of 3
Supported ionic liquids to purify microbial L-Asparaginase
Almeida, Mafalda R.; Nunes, João C. F.; Pereira, Matheus M.; Bento, Heitor B. S.; Pedrolli, Danielle B.; Santos-Ebinuma, Valéria C.; Neves, Márcia C.L-Asparaginase (ASNase) is a versatile enzyme that converts L-asparagine into ammonia and aspartic acid. This enzyme has applications in the food industry and health sector. However, high purity ASNase is required, resulting in high production costs. Therefore, in this work, two supported ionic liquids (SILs), specifically silica modified with dimethylbutylpropylammonium chloride ([Si][N3114]Cl) or triethylprop...
Designing protein nano-construct in ionic liquid: a boost in efficacy of cytoch...
Thayallath, Sarath Kumar; Shet, Sachin M.; Bisht, Meena; Bharadwaj, Pranav; Pereira, Matheus M.; Franklin, Gregory; Nataraj, S. K.; Mondal, DibyenduHerein, we present a simple approach to fabricate protein nanoconstructs by complexing cytochrome C (Cyt C) with silk nanofibrils (SNF) and choline dihydrogen phosphate ionic liquid (IL). The peroxidase activity of the IL modified Cyt C nanoconstruct (Cyt C + SNF + IL) increased significantly (2.5 to 10-fold) over unmodified Cyt C and showed enhanced catalytic activity and stability under harsh conditions, prov...
Production of Human Milk Fat Substitutes by Lipase-Catalyzed Acidolysis: Immobi...
Soares, Cleide M. F.; Barbosa, Milson S.; Santos, Samuel B.; Mattedi, Silvana; Lima, Álvaro S.; Pereira, Matheus M.; Tecelão, CarlaHuman milk fat (HMF) triacylglycerols (TAGs) mainly contain palmitic acid esterified at the sn-2 position while oleic and other unsaturated fatty acids are located at positions sn-1,3. This study aimed at the production of HMF substitutes (HMFS) by lipase-catalyzed acidolysis of tripalmitin with oleic acid, in a solvent-free medium. Burkholderia cepacia lipase (BCL) was immobilized in silica (prepared with prot...
Multimodal ionic liquid-based chromatographic supports for an effective RNA pur...
Carapito, Rita; Bernardo, Sandra C.; Pereira, Matheus M.; Neves, Márcia C.; Freire, Mara G.; Sousa, FaniNucleic acids have been considered interesting molecules to be used as biopharmaceuticals for the treatment of various diseases, in gene therapy strategies. In particular, RNA arises as the most promising approach because it does not require access to the nucleus of cells to exert its function; however, it is quite challenging due to its labile nature. To increase the possibility of translating RNA-based techno...
Multi-walled carbon nanotubes as a platform for Immunoglobulin G attachment
Almeida, Mafalda R.; Barros, Rita A.M.; Pereira, Matheus M.; Castro, Daniel; Faria, Joaquim L.; Freire, Mara G.; Silva, Cláudia G.; Tavares, Ana P.M.Nanomaterials have been extensively used in different applications due to their peculiar characteristics and nanoscale dimensions. Among nanoparticles, carbon-based nanomaterials are becoming highly attractive for biomedical applications such as diagnosis, tissue engineering, drug delivery, and biosensing. The conjugation of carbon-based nanomaterials with antibodies combines the properties of these materials w...
Multimodal ionic liquid-based chromatographic supports for an effective RNA pur...
Carapito, Rita; Bernardo, Sandra C.; Pereira, Matheus M.; Neves, Márcia C.; Freire, Mara G.; Sousa, FaniNucleic acids have been considered interesting molecules to be used as biopharmaceuticals for the treatment of various diseases, in gene therapy strategies. In particular, RNA arises as the most promising approach because it does not require access to the nucleus of cells to exert its function; however, it is quite challenging due to its labile nature. To increase the possibility of translating RNA-based techno...
Production of human milk fat substitutes by lipase-catalyzed acidolysis: immobi...
Soares, Cleide M. F.; Barbosa, Milson S.; Santos, Samuel B.; Mattedi, Silvana; Lima, Álvaro S.; Pereira, Matheus M.; Tecelão, CarlaHuman milk fat (HMF) triacylglycerols (TAGs) mainly contain palmitic acid esterified at the sn-2 position while oleic and other unsaturated fatty acids are located at positions sn-1,3. This study aimed at the production of HMF substitutes (HMFS) by lipase-catalyzed acidolysis of tripalmitin with oleic acid, in a solvent-free medium. Burkholderia cepacia lipase (BCL) was immobilized in silica (prepared with prot...
Multimodal ionic liquid-based chromatographic supports for an effective RNA pur...
Carapito, Ana Rita Mugeiro; Bernardo, Sandra C.; Pereira, Matheus M.; Neves, Márcia C.; Freire, Mara G.; Sousa, FaniNucleic acids have been considered interesting molecules to be used as biopharmaceuticals for the treatment of various diseases, in gene therapy strategies. In particular, RNA arises as the most promising approach because it does not require access to the nucleus of cells to exert its function; however, it is quite challenging due to its labile nature. To increase the possibility of translating RNA-based techno...
Enhanced Enzyme Reuse through the Bioconjugation of L-Asparaginase and Silica-B...
Nunes, João C. F.; Almeida, Mafalda R.; Bento, Rui M. F.; Pereira, Matheus M.; Santos-Ebinuma, Valéria C. [UNESP]; Neves, Márcia C.; Freire, Mara G.Made available in DSpace on 2022-04-28T19:50:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-02-01; L-asparaginase (ASNase) is an amidohydrolase that can be used as a biopharmaceutical, as an agent for acrylamide reduction, and as an active molecule for L-asparagine detection. However, its free form displays some limitations, such as the enzyme’s single use and low stability. Hence, immobilization is ...
Integrated approach to extract and purify proteins from honey by ionic liquid-b...
Pereira, Matheus M.; Pedro, Sónia N.; Quental, Maria V.; Mohamadou, Aminou; Coutinho, João A. P.; Freire, Mara G.The purification of value-added compounds by three phase partitioning (TPP) is a promising alternative to conventional processes since the target compound can be easily recovered from the liquid-liquid interphase. Although this technique has been successfully applied to the recovery of proteins, the minimization of the use of salts and solvents must be pursued to improve the overall process sustainability. Acco...