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Solubilization and hydrolysis of porcine coagulated blood protein using sub-cri...

Marques, Bianca; Nunes, Rafaela; Araújo-Rodrigues, Helena; Pintado, Manuela; Pereira, Ricardo N.; Teixeira, José A.; Rocha, Cristina M. R.

Pork represents a major fraction of the meat consumed worldwide but only 30% of the blood generated in slaughterhouses is re-used as raw material for food and feed. Innovative technologies and efficient processing strategies capable of generating added-value products from it are now attracting attention. In this study, the hydrolysis of porcine coagulated blood using sub-critical solvent extraction was investig...


Solubilization and hydrolysis of porcine coagulated blood protein using sub-cri...

Marques, Bianca; Nunes, Rafaela; Araújo-Rodrigues, Helena; Pintado, Manuela; Pereira, Ricardo N.; Teixeira, J. A.; Rocha, Cristina M.R.

Pork represents a major fraction of the meat consumed worldwide but only 30% of the blood generated in slaughterhouses is re-used as raw material for food and feed. Innovative technologies and efficient processing strategies capable of generating added-value products from it are now attracting attention. In this study, the hydrolysis of porcine coagulated blood using sub-critical solvent extraction was investig...


Comparison of different pretreatment processes envisaging the potential use of ...

Marques, Fabiana; Pereira, Francisco; Machado, Luís; Martins, Joana T.; Pereira, Ricardo N.; Costa, Monya; Genisheva, Zlatina; Pereira, Hugo

A significant fraction of the food produced worldwide is currently lost or wasted throughout the supply chain, squandering natural and economic resources. Food waste valorization will be an important necessity in the coming years. This work investigates the ability of food waste to serve as a viable nutritional substrate for the heterotrophic growth of Chlorella vulgaris. The impact of different pretreatments o...


Enzymatic approach for the extraction of bioactive fractions from red, green an...

Teixeira-Guedes, Catarina; Gomes-Dias, Joana S.; Cunha, Sara A.; Pintado, Manuela E.; Pereira, Ricardo N.; Teixeira, José A.; Rocha, Cristina M. R.

Although several enzymatic approaches have been applied with this intent, the sequential use of enzymes covering both cellulolytic and proteolytic activity has never been performed in seaweeds. Sequential use of these enzymes improved the overall extraction yield by up to 160%, 30% and 80% in the different seaweeds when compared to the control condition (water extraction), use of a carbohydrases’ cocktail alone...


Combining high pressure and electric fields towards nannochloropsis oculata eic...

Sousa, Sérgio; Carvalho, Ana P.; Pinto, Carlos A.; Amaral, Renata A.; Saraiva, Jorge A.; Pereira, Ricardo N.; Vicente, António A.; Freitas, Ana C.

Nannochloropsis oculata is naturally rich in eicosapentaenoic acid (EPA). To turn this microalga into an economically viable source for commercial applications, extraction efficiency must be achieved. Pursuing this goal, emerging technologies such as high hydrostatic pressure (HHP) and moderate electric fields (MEF) were tested, aiming to increase EPA accessibility and subsequent extraction yields. The innovati...


Influence of ohmic heating on lentil protein structure and protein-pectin inter...

Miranda, Cristiane Grella; Rodrigues, Rui M.; Pereira, Ricardo N.; Speranza, Paula; Kurozawa, Louise Emy; Vicente, A. A.; Sato, Ana Carla Kawazoe

Proteins are essential for creating structured food systems due to their interactions with other molecules, but their structure is highly affected by temperature. This study aimed to evaluate the effects of different thermal processes (conventional and ohmic heating) on lentil protein structure and its interactions with pectin. The results showed that thermal processes can modify lentil protein structure, with ...


Brewer's yeast as a clean label ingredient for replacement of phosphates in hams

Tomaz, A Samuel; Machado Jr, Júlio C.; Gomes-Dias, Joana S.; Pereira, Ricardo N.; Rocha, Cristina M. R.; Ferreira, Isabel M.P.L.V.O.


Bioactivity and bioaccessibility of bioactive compounds in gastrointestinal dig...

Coelho, Marta; Oliveira, Carla; Coscueta, Ezequiel R.; Fernandes, João; Pereira, Ricardo N.; Teixeira, José A.; Rodrigues, António Sebastião

A nutrient-rich diet is a key to improving the chemical signals, such as antioxidants, which modulate pathogens’ resistance in the gut and prevent diseases. A current industrial problem is the generation of undervalued by-products, such as tomato bagasse, which are rich in bioactive compounds and of commercial interest (carotenoids and phenolic compounds). This work analyzed the effect of gastrointestinal diges...


Anthocyanin recovery from grape by-products by combining ohmic heating with foo...

Coelho, Marta; Silva, Sara; Costa, Eduardo; Pereira, Ricardo N.; Rodrigues, António Sebastião; Teixeira, José António; Pintado, Manuela

Usually, wine-making by-products are discarded, presenting a significant environmental impact. However, they can be used as a source of bioactive compounds. Moreover, consumers’ increasing demand for naturally nutritious and healthy products requires new formulations and food product improvement, together with sustainable, environmentally friendly extraction methods. Thus, this work aimed to compare ohmic heati...


Influence of ohmic heating on the structural and immunoreactive properties of s...

Pereira, Ricardo N.; Rodrigues, Rui Miguel Martins; Machado, Luís Pedro Lima; Ferreira, Sara; Costa, Joana; Villa, Caterina; Barreiros, Mariana

Ohmic heating (OH) encompasses interesting benefits towards thermal processing. Envisaging an increasing relevance of soybean protein as an alternative non-animal protein, it is important to understand how OH can contribute to the quality and immunoreactivity of soybean-derived products. This study describes, for the first time, the impact of OH when applied at different electrical frequencies (50 Hz20 kHz) and...


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