6 documents found, page 1 of 1
β-carotene and resveratrol loaded glycerol monostearate-based oleogels: Physico...
Barroso, Noádia G.; Martins, Artur J.; Júnior, Fernando D. O.; Okuro, Paula K.; Pereira, Rui C.; Vicente, A. A.; Pastrana, Lorenzo M.; Cunha, Rosiane L.Oleogels are semi-solid systems that can function both as replacers of trans and saturated fats and/or as carriers of lipophilic bioactive compounds. However, bioactive compounds can affect the structure of the oleogel matrix and this effect depends on the properties of such compounds. Therefore, the aim of this study was to develop oleogels loaded with -carotene (BC) or resveratrol (R), with low concentrations...
Encapsulation and survival of a chondrocyte cell line within xanthan gum deriva...
Mendes, Ana Carina; Baran, Erkan Türker; Pereira, Rui C.; Azevedo, Helena S.; Reis, R. L.A chemical derivative of xanthan gum polysaccharide is investigated as a new artificial matrix for the encapsulation of chondrocytic cells. Toward this goal, a novel micro-droplet generator is developed to produce microcapsules. Microcapsules with an average diameter of 500 mm, smooth surface, and homogeneous size distribution are obtained. ATDC5 cells encapsulated in carboxymethyl xanthan (CMX) microcapsules r...
Semantic annotation of biological concepts interplaying microbial cellular resp...
Carreira, Rafael; Carneiro, S.; Pereira, Rui C.; Rocha, Miguel; Rocha, I.; Ferreira, Eugénio C.; Lourenço, AnáliaBackground Automated extraction systems have become a time saving necessity in Systems Biology. Considerable human effort is needed to model, analyse and simulate biological networks. Thus, one of the challenges posed to Biomedical Text Mining tools is that of learning to recognise a wide variety of biological concepts with different functional roles to assist in these processes. Results Here, we present a nove...
Encapsulation of human articular chondrocytes into 3D hydrogel: phenotype and g...
Pereira, Rui C.; Gentili, Chiara; Cancedda, Ranieri; Azevedo, Helena S.; Reis, R. L.This chapter is intended to provide a summary of the current materials used in cell encapsulation technology as well as methods for evaluating the performance of cells encapsulated in a polymeric matrix. In particular, it describes the experimental procedure to prepare a hydrogel matrix based on natural polymers for encapsulating and culturing human articular chondrocytes with the interest in cartilage regenera...
Chitosan scaffolds incorporating lysozyme into CaP coatings produced by a biomi...
Martins, Ana M.; Pereira, Rui C.; Leonor, I. B.; Azevedo, Helena S.; Reis, R. L.This study describes an innovative self-regulated degrading material with gradual in situ pore formation ability for bone tissue engineering applications. This approach is based on the incorporation of the lysozyme enzyme into calcium phosphate (CaP) coatings, prepared on the surface of chitosan scaffolds by means of a biomimetic coating technique with the aim of controlling their degradation rate and subsequen...
Novel injectable gel (system) as a vehicle for human articular chondrocytes in ...
Pereira, Rui C.; Scaranari, M.; Castagnola, P.; Grandizio, M.; Azevedo, Helena S.; Reis, R. L.; Cancedda, Ranieri; Gentili, ChiaraWe developed a novel injectable carrageenan/fibrin/hyaluronic acid-based hydrogel with in situ gelling properties to be seeded with chondrogenic cells and used for cartilage tissue engineering applications. We first analysed the distribution within the hydrogel construct and the phenotype of human articular chondrocytes (HACs) cultured for 3 weeks in vitro. We observed a statistically significant increase in th...