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Analysis of Tc1-Mariner elements in Sclerotinia sclerotiorum suggests recent ac...
Santana, Mateus F.; Silva, José C. F.; Mizubuti, Eduardo S. G.; Araújo, Elza F.; Queiroz, Marisa V.Sclerotinia sclerotiorum is a necrotrophic fungus that is pathogenic to many plants. Genomic analysis of its revealed transposable element expansion that has strongly influenced the evolutionary trajectory of several species. Transposons from the Tc1-Mariner superfamily are thought to be ubiquitous components of fungal genomes and are generally found in low copy numbers with large numbers of deleterious mutatio...
Polymorphism in the internal transcribed spacer (ITS) of the ribosomal DNA of 2...
Gomes, Eliane A.; Kasuya, Maria Catarina M.; Barros, Everaldo G. de; Borges, Arnaldo C.; Araújo, Elza F.Inter- and intraspecific variation among 26 isolates of ectomycorrhizal fungi belonging to 8 genera and 19 species were evaluated by analysis of the internal transcribed sequence (ITS) of the rDNA region using restriction fragment length polymorphism (RFLP). The ITS region was first amplified by polymerase chain reaction (PCR) with specific primers and then cleaved with different restriction enzymes. Amplificat...
PCR amplification and sequence analyses of Reverse Transcriptase-like genes in ...
Pereira, Jorge F.; Ignacchiti, Mariana D. C.; Araújo, Elza F.; Brommonschenkel, Sérgio H.; Cascardo, Júlio C. M.; Pereira, Gonçalo A. G.A análise de sequências de transcriptase reversa (RT) é uma etapa importante para descobrir a presença de elementos transponíveis e investigar o seu papel na geração de variabilidade genética em C. perniciosa. Seqüências putativas de TR foram analisadas no genoma do fitopatógeno C. perniciosa, o agente causal da doença vassoura-de-bruxa no cacau. Um fragmento de 394 pb foi amplificado a partir do DNA genômico d...
The glyceraldehyde-3-phosphate dehydrogenase gene of Moniliophthora perniciosa,...
Lima, Juliana O.; Pereira, Jorge F.; Rincones, Johana; Barau, Joan G.; Araújo, Elza F.; Pereira, Gonçalo A. G.; Queiroz, Marisa V.This report describes the cloning, sequence and expression analysis of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene of Moniliophthora perniciosa, the most important pathogen of cocoa in Brazil. Southern blot analysis revealed the presence of a single copy of the GAPDH gene in the M. perniciosa genome (MpGAPDH). The complete MpGAPDH coding sequence contained 1,461 bp with eight introns that were con...
Small heat shock proteins in the development of thermotolerance in Pisolithus sp.
Ferreira, Adão S.; Kasuya, Maria C. M.; Araújo, Elza F.; Borges, Arnaldo C.; Tótola, Marco. R.Small heat shock proteins (HSPs) have been shown to confer thermotolerance in many organisms. Here, we demonstrate that small HSPs (sHSPs) can also be involved in development of thermotolerance in Pisolithus sp. In heat shock response, Pisolithus isolate RV82 synthesized proteins of molecular mass 28, 26 and 15–18 kDa. These group of proteins are synthesized when mycelial mass are exposed to heat shock temperat...
MpSaci is a widespread gypsy-Ty3 retrotransposon highly represented by non-auto...
Araújo, Elza F.; Queiroz, Casley B.; Queiroz, Marisa V.; Pereira, Jorge F.; Brommonschenkel, Sérgio H.; Costa, Gustavo G. L.; Carazzolle, Marcelo F.Transposons are an important source of genetic variation. The phytopathogen Moniliophthora perniciosa shows high level of variability but little is known about the role of class I elements in shaping its genome. In this work, we aimed the characterization of a new gypsy/Ty3 retrotransposon species, named MpSaci, in the M. perniciosa genome. These elements are largely variable in size, ranging from 4 to 15 kb, a...
Fermentation of maltose and starch by Klebsiella oxytoca P2
Santos, Vera L. dos; Guimarães, Walter V.; Barros, Everaldo G. de; Araújo, Elza F.Klebsiella oxytoca P2, which has genes from Zymomonas mobilis encoding the alcohol dehydrogenase and pyruvate decarboxylase integrated in its chromosome, fermented 50 g maltose/l to 25.4 g of ethanol/l. It also fermented 10, 20 and 40 g starch/l yielding 4, 8.4, and 17.7 g ethanol/l, respectively, representing 72, 75 and 78% of the theoretical yield.
The glyceraldehyde-3-phosphate dehydrogenase gene of Moniliophthoraperniciosa, ...
Lima, Juliana O.; Pereira, Jorge F.; Rincones, Johana; Barau, Joan G.; Araújo, Elza F.; Pereira, Gonçalo A.G.; Queiroz, Marisa V.This report describes the cloning, sequence and expression analysis of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene of Moniliophthora perniciosa, the most important pathogen of cocoa in Brazil. Southern blot analysis revealed the presence of a single copy of the GAPDH gene in the M. perniciosa genome (MpGAPDH). The complete MpGAPDH coding sequence contained 1,461 bp with eight introns that were con...