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Impact of Non-Saccharomyces Yeast Fermentation in Madeira Wine Chemical Composi...

Miranda, Andreia; Pereira, Vanda; Jardim, Humberto; Malfeito-Ferreira, Manuel; Marques, José Carlos

Madeira wine is produced via spontaneous alcoholic fermentation arrested by ethanol addition. The increasing demand of the wine market has led to the need to standardize the winemak ing process. This study focuses on identifying the microbiota of indigenous yeasts present during Madeira wine fermentation and then evaluates the impact of selected indigenous non-Saccharomyces as pure starter culture (Hanseniaspor...


Impact of Non-Saccharomyces yeast fermentation in Madeira wine chemical composi...

Miranda, Andreia; Pereira, Vanda; Jardim, Humberto; Malfeito-Ferreira, Manuel; Marques, José Carlos

Madeira wine is produced via spontaneous alcoholic fermentation arrested by ethanol addition. The increasing demand of the wine market has led to the need to standardize the winemaking process. This study focuses on identifying the microbiota of indigenous yeasts present during Madeira wine fermentation and then evaluates the impact of selected indigenous non-Saccharomyces as pure starter culture (Hanseniaspora...


Unveiling the evolution of Madeira Wine key metabolites: a three-year follow-up...

Pereira, Vanda; Leça, João M.; Freitas, Ana I.; Pereira, Ana C.; Pontes, Marisela; Albuquerque, Francisco; Marques, José C.

Madeira wine (MW) encompasses an unusual oxidative ageing process that makes it distinc tive. Several metabolites have been related to its quality and safety, such as 5-hydroxymethylfurfural (HMF), furfural, sotolon, and ethyl carbamate (EC). These compounds were quantified over a three year period to assess their formation rate according to the ageing procedure used: canteiro vs. estufagem. Estufagem, which in...


Real-Time Measurement of Refractive Index Using 3D-Printed Optofluidic Fiber Se...

Leça, João M.; Magalhães, Yannis; Antunes, Paulo; Pereira, Vanda; Ferreira, Marta S.

This work describes a 3D-printed optofluidic fiber sensor to measure refractive index in real time, combining a microfluidic system with an optical fiber extrinsic Fabry-Perot interferometer. The microfluidic chip platform was developed for this purpose through 3D printing. The Fabry-Perot cavity was incorporated in the microfluidic chip perpendicularly to the sample flow, which was of approximately 3.7 µL/s. T...


Optical Fiber Sensor for Monitoring the Evaporation of Ethanol-Water Mixtures

Pereira, Diana; Bierlich, Jörg; Kobelke, Jens; Pereira, Vanda; Ferreira, Marta S.

An inline optical fiber sensor is proposed to monitor in real time the evaporation process of ethanol-water binary mixtures. The sensor presents two interferometers, a cladding modal interferometer (CMI) and a Mach-Zehnder interferometer (MZI). The CMI is used to acquire the variations in the external medium refractive index, presenting a maximum sensitivity of 387 nm/RIU, and to attain the variation in the sam...


New insights into ethyl carbamate occurrence in fortified wines

Leça, João M.; Pereira, Vanda; Miranda, Andreia; Vilchez, José Luis; Marques, José C.

The occurrence of ethyl carbamate (EC) in fortified wines was studied testing different model wines under accelerated ageing (45 °C for 4 months and 70 °C for 1 month), to investigate the possibility of arginine (Arg) being a direct precursor of EC in fortified wines, comparing it with known major precursors, urea and citrulline (Cit). Wine main sugars were appraised as possible catalysts, as previously pointed...


Impact of indigenous non-saccharomyces yeasts isolated from Madeira Island vine...

Leça, João Micael; Pereira, Vanda; Miranda, Andreia; Vilchez, José Luis; Malfeito-Ferreira, Manuel; Marques, José Carlos

The impact of selected non-Saccharomyces yeasts on the occurrence of ethyl carbamate (EC) was evaluated. Hanseniaspora uvarum, Starmerella bacillaris, Pichia terricola, Pichia fermentans and Pichia kluyveri isolated from Madeira Island vineyards were inoculated in Tinta Negra musts. Urea, citrulline (Cit) and arginine (Arg) were quantified when the density of musts attained the levels to obtain sweet (1052 ± 5 ...


Impact of indigenous non-Saccharomyces yeasts isolated from Madeira Island vine...

Leça, João Micael; Pereira, Vanda; Miranda, Andreia; Vilchez, José Luís; Malfeito-Ferreira, Manuel

The impact of selected non-Saccharomyces yeasts on the occurrence of ethyl carbamate (EC) was evaluated. Hanseniaspora uvarum, Starmerella bacillaris, Pichia terricola, Pichia fermentans and Pichia kluyveri isolated from Madeira Island vineyards were inoculated in Tinta Negra musts. Urea, citrulline (Cit) and arginine (Arg) were quantified when the density of musts attained the levels to obtain sweet (1052 5 g/...


Is sotolon relevant to the aroma of Madeira Wine blends?

Gaspar, João M.; Freitas, Ana I.; Zhao, Qianzhu; Leça, João M.; Pereira, Vanda; Marques, José C.

Madeira wine (MW) oxidative aging results in the formation of several key aromas. Little is still known about their odor relevance to the aroma of the most commercialized MWs. This report presents an in-depth study of the odor impact of sotolon in MW blends. First, its odor perception was estimated in MWs according to ASTM E679, testing different 3-year-old (3-yo) commercial blends. The odor relevance of sotolo...


Evaluation of fucoxanthin contents in seaweed biomass by vortex-assisted solid-...

Nunes, N.; Leça, João M.; Pereira, Ana C.; Pereira, Vanda; Ferraz, S.; Barreto, Maria Carmo; Marques, José C.; Carvalho, M.A.A. Pinheiro de

Fucoxanthin is considered an important marine bioactive compound with biological properties with promising effects, namely on health. A simple and efficient analytical methodology is proposed for its quantification in seaweed biomass by using vortex-assisted solid-liquid microextraction (VASLME) followed by reversed phase high-performance liquid chromatography (RP-HPLC) photodiode array detection (PDA) analysis...


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