2 documents found, page 1 of 1
Polymerase chain reaction and nested-PCR approaches for detecting Cryptosporidi...
Osaki,Silvia Cristina; Soccol,Vanete Thomaz; Costa,Adriana Oliveira; Oliveira-Silva,Marcia Benedita; Pereira,Juliana Tracz; Procopio,Antonio EduardoIntroduction Cryptosporidium is an important protozoan cause of waterborne disease worldwide of concern to public health authorities. To prevent outbreaks of cryptosporidiosis, the monitoring of this parasite in drinking water is necessary. In the present work, the polymerase chain reaction (PCR) and nested-PCR techniques were used to detect Cryptosporidium in raw water from catchment points of four water treat...
Production of anti-Cryptosporidium polyclonal antibodies and standardization of...
Osaki,Silvia Cristina; Costa,Adriana Oliveira; Troiano,Ludmilla Della Coletta; Kruger,Ernesto Renato; Pereira,Juliana Tracz; Fernandes,Nelson Luis MelloINTRODUCTION: The production of anti-Cryptosporidium polyclonal antibodies and its use in direct immunofluorescence assays to determine the presence of Cryptosporidium in water are described in the present work. METHODS: Two rabbits were immunized with soluble and particulate antigens from purified Cryptosporidium oocysts. The sera produced were prepared for immunoglobulin G extraction, which were then purified...