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Growth, oxidative stress, and intermediary metabolism of European seabass (Dice...

Filipe, Diogo; Magalhães, Rui; Fernandes, Helena; Salgado, José Manuel Seara; Belo, Isabel; Oliva-Teles, Aires; Peres, Helena

Previous research demonstrated that solid-state fermentation (SSF) of distiller's dried grains with solubles (DDGS) by Aspergillus ibericus improved protein, reduced neutral and acid detergent fiber levels, and added phenolic compounds, xylanase, and cellulase. Fermentation of DDGS also increased protein, lipids, energy, and starch digestibility. This study evaluated the effects of unfermented and fermented DDG...


Effects of solid-state fermentation of Gelidium corneum by-product on immune st...

Ferreira, Marta Alexandra Lages; Ramos-Oliveira, Catarina; Magalhães, Rui; Martins, Nicole; Serra, Cláudia R.; Salgado, José Manuel Seara; Belo, Isabel

It was previously observed that dietary inclusion of 10 % Gelidium corneum by-product and sunflower cake mixture (1:1 ratio; Gmix), replacing corn gluten meal, reduced feed utilization efficiency in European sea bass juveniles, while the same mixture fermented with Aspergillus ibericus restored feed efficiency as well as nitrogen and energy utilization, achieving levels comparable to the control diet. The prese...


Effect of solid-fermented brewers spent grain on growth, metabolism, and oxidat...

Estevão-Rodrigues, Tássia; Fernandes, Helena; Moutinho, Sara; Ferreira, Marta Alexandra Lages; Castro, Carolina; Belo, Isabel; Salgado, José Manuel

Replacing traditional agricultural ingredients with biotechnologically improved agro-industry by-products in fish diets promotes sustainable aquaculture, reduces production costs and carbon footprint, and promotes a circular economy. Brewers spent grain (BSG) is one such by-product. Solid-state fermentation (SSF) of BSG with Aspergillus ibericus enhances its nutritional value and digestibility for European seab...


Effectiveness of high-solid loading treatments to enhance nutrient and antioxid...

Ramos-Oliveira, Catarina; Ferreira, Marta Alexandra Lages; Belo, Isabel; Oliva-Teles, Aires; Peres, Helena

Macroalgae have low nutrient bioavailability, often requiring pretreatmentsphysical, chemical, or biologicaltypically using low-solid loading hydrolysis, which produces separate liquid and solid phases. In contrast, high-solid loading hydrolysis offers a single-phase alternative, though it remains underexplored for macroalgae. This study evaluated the effectiveness of high-solid loading hydrolysis for breaking ...


Aspergillus ibericus biomass production by submerged fermentation of Codium tom...

Ferreira, Marta Alexandra Lages; Salgado, José Manuel Seara; Peres, Helena; Belo, Isabel

Seaweeds are potential feedstocks for biorefinery approaches, owing to their carbohydrate content, high biomass production and lack of lignin content, which makes saccharification more feasible. The seaweed sugar-rich hydrolysates can be used to produce alternative protein sources, such as mycoprotein, to meet the increasing global protein needs in a sustainable way. Therefore, this study aimed to produce bioma...


Fermented agar by-product and sunflower cake mixture as feedstuff for European ...

Ferreira, Marta; Ramos-Oliveira, Catarina; Magalhães, Rui; Martins, Nicole; Ozório, Rodrigo O. A.; Salgado, José Manuel Seara; Belo, Isabel

Industrial agar production from red seaweeds such as Gelidium sp. generates large quantities of byproducts (GBP), often discarded as waste. GBP and sunflower cake (SC) are under-valorized as feedstuff due to their high non-starch polysaccharide (NSP) content. Solid-state fermentation (SSF) may disrupt NSP and improve their nutritional value. This study evaluated the utilization of Aspergillus ibericus and Asper...


Valorization of Gelidium corneum by-product through solid-state fermentation

Ferreira, Marta Alexandra Lages; Salgado, José Manuel Seara; Peres, Helena; Belo, Isabel

Red seaweed Gelidium is used for agar extraction, generating large quantities of by-products (red seaweed by-product RSB). Its carbohydrate and protein-rich composition makes it a suitable substrate to produce value-added compounds through solid-state fermentation (SSF). In this study, RSB was used in SSF with Aspergillus ibericus and Aspergillus niger for carbohydrases production and protein enrichment. RSB wa...


Effect of solid-state fermentation of brewer's spent grain on digestibility and...

Estevão-Rodrigues, Tássia; Fernandes, Helena; Moutinho, Sara; Filipe, Diogo; Fontinha, Filipa; Magalhães, Rui; Couto, Ana; Ferreira, Marta

This study assessed the effects of dietary inclusion of solid-state fermented (SSF) brewers spent grain (BSG) with Aspergillus ibericus on nutrient and energy digestibility, digestive enzyme activity, and intestinal histomorphology of juvenile European seabass (Dicentrarchus labrax). Five diets (18% crude lipids and 45% crude protein) were formulated, including a control diet (without BSG), two diets with 10% a...


Solid-state fermentation of distiller’s dried grains with solubles improves dig...

Filipe, Diogo; Dias, Mário; Magalhães, Rui; Fernandes, Helena; Salgado, José; Belo, Isabel; Oliva-Teles, Aires; Peres, Helena

Aquaculture requires new, economical, and eco-friendly protein sources to replace traditional fisheries and plant ingredients. Using agriculture by-products as protein sources would reduce land-based feed production pressure and waste production, promoting a circular economy and sustainable aquaculture. Distiller’s dried grains with solubles (DDGS) is the main by-product of bioethanol production. Corn DDGS has ...


Shelf-life performance of fish feed supplemented with bioactive extracts from f...

Filipe, Diogo; Gonçalves, A.; Fernandes, Helena; Oliva-Teles, Aires; Peres, Helena; Belo, Isabel; Salgado, José Manuel

Fortifying fish feeds with bioactive compounds, such as enzymes and antioxidants, has been an adopted strategy to improve feed nutritional quality and sustainability. However, feed additives can lose activity/effectiveness during pelleting and storage processes. This work aimed to monitor functional activity stability in feeds supplemented with a bioactive extract, including cellulases, xylanases, and antioxida...


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