9 documents found, page 1 of 1
Silk sericin/chitosan supramolecular multilayered thin films as sustainable cyt...
Rosas, Miguel; Sousa, Cristiana F. V.; Pereira, Ana; Amaral, Adérito J. R.; Pesqueira, Tamagno; Patrício, Sónia G.; Fateixa, SaraSilk sericin (SS) has been widely discarded as a waste by the silk textile industry during the degumming process to obtain fibroin. However, in the past decade, an in-depth understanding of its properties and functions turned it into a high added-value biomaterial for biomedical applications. Herein, we report the molecular design and development of sustainable supramolecular multilayered nanobiomaterials encom...
Bioinstructive Layer-by-Layer-Coated Customizable 3D Printed Perfusable Microch...
Sousa, Cristiana F. V.; Saraiva, Catarina A.; Correia, Tiago R.; Pesqueira, Tamagno; Patrício, Sónia G.; Rial-Hermida, Maria Isabel; Borges, JoãoThe development of complex and large 3D vascularized tissue constructs remains the major goal of tissue engineering and regenerative medicine (TERM). To date, several strategies have been proposed to build functional and perfusable vascular networks in 3D tissue-engineered constructs to ensure the long-term cell survival and the functionality of the assembled tissues after implantation. However, none of them ha...
Enzymatically degradable, starch-based layer-by-layer films: application to cyt...
Moon, Hee Chul; Han, Sol; Borges, João; Pesqueira, Tamagno; Choi, Hyunwoo; Han, Sang Yeong; Cho, Hyeoncheol; Park, Ji Hun; Mano, João F.The build-up and degradation of cytocompatible nanofilms in a controlled fashion have great potential in biomedical and nanomedicinal fields, including single-cell nanoencapsulation (SCNE). Herein, we report the fabrication of biodegradable films of cationic starch (c-ST) and anionic alginate (ALG) by electrostatically driven layer-by-layer (LbL) assembly technology and its application to the SCNE. The [c-ST/AL...
The effects of platelet lysate patches on the activity of tendon-derived cells
Almeida, Raquel Carvalho Ferreira Costa; Franco, Albina Ribeiro; Pesqueira, Tamagno; Oliveira, Mariana Braga; Babo, Pedro S.; Leonor, I. B.; Mano, J. F.Platelet-derived biomaterials are widely explored as cost-effective sources of therapeutic factors, holding a strong potential for endogenous regenerative medicine. Particularly for tendon repair, treatment approaches that shift the injury environment are explored to accelerate tendon regeneration. Herein, genipin-crosslinked platelet lysate (PL) patches are proposed for the delivery of human-derived therapeuti...
Magnetotherapy: the quest for tendon regeneration
Pesqueira, Tamagno; Almeida, Raquel Carvalho Ferreira Costa; Gomes, Manuela E.Tendons are mechanosensitive tissues that connect and transmit the forces generated by muscles to bones by allowing the conversion of mechanical input into biochemical signals. These physical forces perform the fundamental work of preserving tendon homeostasis assuring body movements. However, overloading causes tissue injuries, which leads us to the field of tendon regeneration. Recently published reviews have...
Engineering magnetically responsive tropoelastin spongy-like hydrogels for soft...
Pesqueira, Tamagno; Almeida, Raquel Carvalho Ferreira Costa; Mithieux, Suzanne M.; Babo, Pedro Miguel Sousa; Franco, Albina Ribeiro; Mendes, Bárbara B.Magnetic biomaterials are a key focus in medical research. Tropoelastin is the soluble precursor of elastin and is a critical component of tissues requiring elasticity as part of their physiological function. By utilising the versatility of tropoelastin and the ability to tailor its properties, we developed a novel magnetic spongy-like hydrogel based on tropoelastin doped with magnetic properties by in situ pre...
A platelet lysate antibacterial bioactive patch for tendon repair
Costa-Almeida, Raquel; Franco, Albina Ribeiro; Leonor, I. B.; Babo, Pedro Miguel Sousa; Pesqueira, Tamagno; Reis, R. L.; Gomes, Manuela E.Platelet lysate (PL) is a class of platelet-rich hemoderivatives produced by cryogenic disruption of platelet concentrates, originating a pool of supra-physiological concentrations of growth factors (GFs) that is being widely explored in the medical field, namely in sports medicine and orthopaedics. In this concern, patch augmentation strategies have been receiving increased attention as the basis for the devel...
Simulated hypergravity induces changes in human tendon-derived cells: from cell...
Costa-Almeida, Raquel; Carvalho, Daniel T. O.; Ferreira, Miguel J. S.; Pesqueira, Tamagno; Monici, Monica; van Loon, Jack J. W. A.; Granja, Pedro L.Gravity influences physical and biological processes, having an impact on development, as well as homeostasis of living systems. The musculoskeletal system is comprised of several mechano- responsive tissues and altered gravitational forces are known to influence distinct properties, including bone mineral density and skeletal muscle mass. This is particularly relevant in a near- weightlessness (microgravity) e...
Magnetically responsive tropoelastin hydrogel as a platform for soft tissue reg...
Pesqueira, Tamagno; Almeida, Raquel Costa; Mithieux, Suzanne M.; Reis, R. L.; Gomes, Manuela E.; Weiss, Anthony S.The natural polymer tropoelastin is a structural protein of ECM of tissues requiring elasticity as part of their function, including ligaments and tendons. Tropoelastin has an innate capacity of self-assembly into high-order structures, and together with elastic resilience, structural stability and bioactivity bring forth pleasant singularities in adopting it as a building block to fabricate hydrogels. Moreover...