2 documents found, page 1 of 1
A Shared Perspective on in Vitro and in Vivo Models to Assay Intestinal Transep...
Hevia, Arancha; Ruas-Madiedo, Patricia; Faria, Miguel Angelo; Petit, Valérie; Alves, Bruna; Alvito, Paula; Arranz, Elena; Bastiaan-Net, ShannaAssessing nutrient bioavailability is complex, as the process involves multiple digestion steps, several cellular environments, and regulatory-metabolic mechanisms. Several in vitro models of different physiological relevance are used to study nutrient absorption, providing significant challenges in data evaluation. However, such in vitro models are needed for mechanistic studies as well as to screen for biolog...
Standardization of in vitro digestibility and DIAAS method based on the static ...
Egger, Lotti; Sousa, Raquel; Recio, Isidra; Abbühl, Lychou; Brügger, Cédric; Hernández, Marta Santos; Macierzanka, Adam; Szumala, PatrycjaBackground: The FAO recommends the digestible indispensable amino acid score (DIAAS) as the measure for protein quality, for which the true ileal digestibility needs to be assessed in humans or pigs. However, due to high costs and ethical concerns, the FAO strongly encourages as well the development of validated in vitro methods, which complement the in vivo experiments. Method: Recently, an in vitro workflow, ...