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Serum-Free Suspension Adaptation of HEK-293T Cells: Basis for Large-Scale Bioph...
Lomba,Ana Luiza Oliveira; Tirapelle,Mariane Cariati; Biaggio,Rafael Tagé; Abreu-Neto,Mário Soares; Covas,Dimas Tadeu; Picanço-Castro,VirginiaAbstract Human Embryonic Kidney 293T cells (HEK-293T) are the most common host for viral vector production and are also widely employed for recombinant protein production. These cells are typically cultured in monolayer (adherent culture) using culture medium containing fetal bovine serum (FBS), which impairs batch-to-batch reproducibility and scale-up. The adaptation of adherent cell culture to suspension cult...
Murine leukemia virus-derived retroviral vector has differential integration pa...
Freitas,Marcela Cristina Correa de; Fontes,Aparecida Maria; Fernandes,Andrielle de Castilho; Picanço-Castro,Virginia; Russo,Elisa Maria de SousaOBJECTIVE: Nowadays recombinant factor VIII is produced in murine cells including in Chinese hamster ovary (CHO) and baby hamster kidney cells (BHK). Previous studies, using the murine leukemia virus-derived retroviral vector pMFG-FVIII-P140K, modified two recombinant human cell lines, HepG2 and Hek293 to produce recombinant factor VIII. In order to characterize these cells, the present study aimed to analyze t...