35 documents found, page 1 of 4

Characterization of an in vitro fed-batch model to obtain cells released from S...

Both dynamic and fed-batch systems have been used for the study of biofilms. Dynamic systems, whose hallmark is the presence of continuous flow, have been considered the most appropriate for the study of the last stage of the biofilm lifecycle: biofilm disassembly. However, fed-batch is still the most used system in the biofilm research field. Hence, we have used a fed-batch system to collect cells released fro...

S. epidermidis biofilm-released cells: the final frontier?

S. epidermidis response to human blood and its cellular and soluble components

Staphylococcus epidermidis, a normal inhabitant of healthy human skin and mucosae, can cause persistent and relapsing infections due to its ability to adhere to medical devices and form biofilms. Hence, S epidermidis is considered one of the most important medical device-associated nosocomial agents, being particularly associated with vascular catheters. Although the biofilms formed on these catheters are in co...

Comparative proteomic and transcriptomic profile of Staphylococcus epidermidis ...

Staphylococcus epidermidis is an important nosocomial agent among carriers of indwelling medical devices, due to its strong ability to form biofilms on inert surfaces. Contrary to some advances made in the transcriptomic field, proteome characterization of S. epidermidis biofilms is less developed. To highlight the relation between transcripts and proteins of S. epidermidis biofilms, we analyzed the proteomic p...

Dormancy within Staphylococcus epidermidis biofilms : a transcriptomic analysis...

The proportion of dormant bacteria within Staphylococcus epidermidis biofilms may determine its inflammatory profile. Previously, we have shown that S. epidermidis biofilms with higher proportions of dormant bacteria have reduced activation of murine macrophages. RNA-sequencing was used to identify the major transcriptomic differences between S. epidermidis biofilms with different proportions of dormant bacteri...

Dormant bacteria within Staphylococcus epidermidis biofilms have low inflammato...

Staphylococcus epidermidis is the most commonly isolated etiological agent of nosocomial infections mainly due to its ability to establish biofilms on indwelling medical devices. Detachment of bacteria from S. epidermidis biofilms and subsequent growth in the planktonic form is a hallmark in the pathogenesis of these infections leading to dissemination. Here we showed that S. epidermidis cells collected from bi...

Alterations in the Staphylococcus epidermidis biofilm transcriptome following i...

Staphylococcus epidermidis biofilm formation on the surface of intravenous catheters is responsible for 22% of the cases of bloodstream infections, in patients in intensive care units in the USA. The ability of S. epidermidis to withstand the high bactericidal activity of human blood is therefore crucial for systemic dissemination. To identify the genes involved in the bacterium's survival, the transcriptome of...

Whole transcriptome analysis of Staphylococcus epidermidis biofilms upon contac...

Staphylococcus epidermidis is commonly associated with foreign body infections due to its ability to form biofilms on the surface of indwelling medical devices. Understanding how the biofilms form on the top of devices and interact with the host, particularly, with human blood components, is of great interest. Here we assessed the whole transcriptome of S. epidermidis biofilms upon contact with human blood. Est...

Monoclonal antibody raised against PNAG has variable effects on static S. epide...

Global transcriptomic analysis of dormancy within Staphylococcus epidermidis bi...