ABSTRACT - The semen quality is one of the determinant factors of ram semen cryopreservation. The present retrospective study aimed to characterize the seasonal ram pattern during the year for ten Portuguese local sheep breeds, hypothesizing that the breed and season had low effects on the main spermatozoa traits. A total of 1471 ejaculates were used and evaluated (fresh semen) from 85 rams between 2004 and 202...
Sperm cells are particularly vulnerable to reactive oxygen species (ROS), impairing their fertilizing ability. Our objective was to study the effect of a novel mitochondrial-directed antioxidant, AntiOxBEN2, on bovine sperm function. This antioxidant was added to the semen capacitation medium (CAP), during the swim-up process, and to the fertilization medium (FERT) during the co-incubation of matured oocytes an...
Endometrium type I (COL1) and III (COL3) collagen accumulation, periglandular fibrosis and mare infertility characterize endometrosis. Metalloproteinase-2 (MMP-2), MMP-9 and tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2) are involved in collagen turnover. Since epigenetic changes may control fibroproliferative diseases, we hypothesized that epigenetic mechanisms could modulate equine endometrosis. ...
Oxidative stress and mitochondrial dysfunction have been associated with the age-related decline of oocyte quality and strategies for their prevention are currently quested. Urolithin A (UA) is a natural metabolite with pro-apoptotic and antioxidant effects, capable of preventing the accumulation of dysfunctional mitochondria in different aged cells. UA has never been tested in bovine oocytes. Our aim was to st...
Este trabalho teve por objetivo avaliar a interação entre a expressão de genes da família priónica (prnp e sprn), e genes ligados à esteroidogenese [CYP11A1(Pregnenolona) e CYP19A1(17-Estradiol)], ao nível de células da granulosa (GCs) de bovinos.
Palavras-chave: Amputação/reabilitação; Lesões dos Dedos/reabilitação; Próteses e Implantes
[Excerto] Introdução: A investigação sobre o papel da escrita no contexto pedagógico demonstra a sua importância não só ao nível da expressão de conhecimento, mas também no que se refere à sua aquisição e elaboração por parte do aprendente. É neste quadro que se fala da escrita como ferramenta de aprendizagem e se realça o contributo que ela pode dar para a promoção de sucesso escolar. Este modo de perspectivar...