Encontrados 11 documentos, a visualizar página 1 de 2
β-micrustoxin (Mlx-9), a PLA2 from Micrurus lemniscatus snake venom: biochemica...
Santos,Natália Fernanda Teixeira dos; Imberg,Andréia de Souza; Mariano,Douglas Oscar Ceolin; Moraes,Angelina Cirelli de; Andrade-Silva,JessicaAbstract Background Endogenous phospholipases A2 (PLA2) play a fundamental role in inflammation, neurodegenerative diseases, apoptosis and cellular senescence. Neurotoxins with PLA2 activity are found in snake venoms from the Elapidae and Viperidae families. The mechanism of action of these neurotoxins have been studied using hippocampal and cerebellar neuronal cultures showing [Ca2+]i increase, mitochondrial d...
Characterization and evaluation of the enzymatic activity of tetanus toxin subm...
Sartori,Giselle Pacifico; Costa,Andréa da; Macarini,Fernanda Lúcio dos Santos; Mariano,Douglas Oscar Ceolin; Pimenta,Daniel CarvalhoAbstract Background Tetanus toxin blocks the release of the inhibitory neurotransmitters in the central nervous system and causes tetanus and its main form of prevention is through vaccination. The vaccine is produced by inactivation of tetanus toxin with formaldehyde, which may cause side effects. An alternative way is the use of ionizing radiation for inactivation of the toxin and also to improve the potentia...
Proteomic characterization of Naja mandalayensis venom
Beraldo Neto,Emídio; Coelho,Guilherme Rabelo; Sciani,Juliana Mozer; Pimenta,Daniel CarvalhoAbstract Background Naja mandalayensis is a spitting cobra from Myanmar. To the best of our knowledge, no studies on this venom composition have been conducted so far. On the other hand, few envenomation descriptions state that it elicits mainly local inflammation in the victims’ eyes, the preferred target of this spiting cobra. Symptoms would typically include burning and painful sensation, conjunctivitis, ede...
Protein identification from the parotoid macrogland secretion of Duttaphrynus m...
Mariano,Douglas Oscar Ceolin; Messias,Marcela Di Giacomo; Spencer,Patrick Jack; Pimenta,Daniel CarvalhoABSTRACT Background: Bufonid parotoid macrogland secretion contains several low molecular mass molecules, such as alkaloids and steroids. Nevertheless, its protein content is poorly understood. Herein, we applied a sample preparation methodology that allows the analysis of viscous matrices in order to examine its proteins. Methods: Duttaphrynus melanostictus parotoid macrogland secretion was submitted to ion-ex...
The modular nature of bradykinin-potentiating peptides isolated from snake venoms
Sciani,Juliana Mozer; Pimenta,Daniel CarvalhoAbstract Bradykinin-potentiating peptides (BPPs) are molecules discovered by Sergio Ferreira – who found them in the venom of Bothrops jararaca in the 1960s – that literally potentiate the action of bradykinin in vivo by, allegedly, inhibiting the angiotensin-converting enzymes. After administration, the global physiological effect of BPP is the decrease of the blood pressure. Due to this interesting effect, on...
Ergosterol isolated from the basidiomycete Pleurotus salmoneostramineus affects...
Alexandre,Tatiana Rodrigues; Lima,Marta Lopes; Galuppo,Mariana Kolos; Mesquita,Juliana Tonini; Nascimento,Matilia Ana do; Santos,Augusto Leonardo dosAbstract Background Major drawbacks of the available treatment against Chagas disease (American trypanosomiasis) include its toxicity and therapeutic inefficiency in the chronic phase of the infection, which makes it a concern among neglected diseases. Therefore, the discovery of alternative drugs for treating chronic Chagas disease requires immediate action. In this work, we evaluated the mushroom Pleurotus sa...
Preliminary molecular characterization of a proinflammatory and nociceptive mol...
Sciani,Juliana Mozer; Zychar,Bianca; Gonçalves,Luis Roberto; Giorgi,Renata; Nogueira,Thiago; Pimenta,Daniel CarvalhoAbstract Background Sea urchins are animals commonly found on the Brazilian shoreline, being Echinometra lucunter the most abundant species. Accidents caused by E. lucunter have been reported as one of the most frequent in Brazil, and are characterized by intense pain and inflammation, consequence of spine puncture in the skin. In order to characterize such toxic effects, we isolated one molecule that caused in...
Crotalus durissus terrificus crotapotin naturally displays preferred positions ...
Oliveira,Laudicéia Alves de; Ferreira Jr,Rui Seabra; Barraviera,Benedito; Carvalho,Francilene Capel Tavares de; Barros,Luciana Curtolo deAbstract Background Classically, Crotalus durissus terrificus (Cdt) venom can be described, according to chromatographic criteria, as a simple venom, composed of four major toxins, namely: gyroxin, crotamine, crotoxin and convulxin. Crotoxin is a non-covalent heterodimeric neurotoxin constituted of two subunits: an active phospholipase A2 and a chaperone protein, termed crotapotin. This molecule is composed of ...
Melittin induces in vitro death of Leishmania (Leishmania) infantum by triggeri...
Pereira,Andreia Vieira; Barros,Gustavo de; Pinto,Erika Gracielle; Tempone,Andre Gustavo; Orsi,Ricardo de Oliveira; Santos,Lucilene Delazari dosAbstract Background Apis mellifera venom, which has already been recommended as an alternative anti-inflammatory treatment, may be also considered an important source of candidate molecules for biotechnological and biomedical uses, such as the treatment of parasitic diseases. Methods Africanized honeybee venom from Apis mellifera was fractionated by RP-C18-HPLC and the obtained melittin was incubated with proma...
Initial peptidomic profiling of Brazilian sea urchins: Arbacia lixula, Lytechin...
Sciani,Juliana Mozer; Emerenciano,Andrews Krupinski; Silva,José Roberto Machado Cunha da; Pimenta,Daniel CarvalhoAbstract Background Sea urchins can be found throughout the Brazilian coast and are reported to be one of the major causes of marine accidents on the shoreline. Although not lethal, these accidents are reported to be extremely painful. In order to understand the toxinology of the Brazilian urchins, a peptidomic approach was performed aiming to characterize the naturally occurring peptides in both the coelomic f...