4 documents found, page 1 of 1
The Centriole Stability Assay
Lince-Faria, Mariana; Silva, Ana; Pimenta-Marques, AnaFunding Information: This protocol was originally published in two research manuscripts by Pimenta-Marques et al. [17,18]. This work was supported by a grant from the Portuguese Research Council (FCT) awarded to APM (PTDC/BIA-BID/32225/2017). Publisher Copyright: © 2025 The Authors; exclusive licensee Bio-protocol LLC.; Centrosomes are vital eukaryotic organelles involved in regulating cell adhesion, polarity, ...
Maintaining centrosomes and cilia
Werner, Sascha; Pimenta-Marques, Ana; Bettencourt-Dias, MónicaCentrosomes and cilia are present in organisms from all branches of the eukaryotic tree of life. These structures are composed of microtubules and various other proteins, and are required for a plethora of cell processes such as structuring the cytoskeleton, sensing the environment, and motility. Deregulation of centrosome and cilium components leads to a wide range of diseases, some of which are incompatible w...
Regulation of Autophosphorylation Controls PLK4 Self-Destruction and Centriole ...
Cunha-Ferreira, Inês; Bento, Inês; Pimenta-Marques, Ana; Jana, Swadhin Chandra; Lince-Faria, Mariana; Duarte, Paulo; Borrego-Pinto, JoanaPolo-like kinase 4 (PLK4) is a major player in centriole biogenesis: in its absence centrioles fail to form, while in excess leads to centriole amplification. The SCF-Slimb/βTrCP-E3 ubiquitin ligase controls PLK4 levels through recognition of a conserved phosphodegron. SCF-Slimb/βTrCP substrate binding and targeting for degradation is normally regulated by phosphorylation cascades, controlling complex processes...
Mitosis and protein Na-terminal acetylation
Pimenta-Marques, AnaDissertation presented to obtain the Ph.D degree in Developmental Biology; Protein N-terminal acetylation is a highly conserved and widespread modification that occurs on approximately 80% of all soluble, cytoplasmic human proteins. Nevertheless, with few exceptions, little is known about the biological function of protein N- acetylation. Recently, it was suggested to act as a general protein destabilization ...