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Sustainable synthesis and optimisation of ethyl oleate from high oleic acid was...

Soares, Ana; Fontes, Ana Luiza; Teixeira, Francisca; Costa, Paula; Pimentel, Lígia; Pintado, Manuela; Rodríguez-Alcalá, Luis Miguel

Ethyl oleate (EO) is a versatile compound with several industrial applications, such as a vaccine adjuvant, an emollient in cosmetics, and a key component in food products as an additive used for pretreatment in preservation processes such as drying, while preserving valuable nutrients. Ethyl oleate is primarily synthesised from edible oils, which raises concerns regarding competition with food production. This...


Evolution of qualitative and quantitative lipid profiles of high-pressure-proce...

Inácio, Rita S.; Rodríguez-Alcalá, Luís; Pimentel, Lígia; Saraiva, Jorge; Gomes, Ana

High-pressure processing (HPP) can be used as a nonthermal pasteurization technique to overcome microbial safety issues of the raw ewes’ milk Serra da Estrela cheese without negatively inf luencing its quality, in particular, the lipid composition partly responsible for Serra da Estrela cheese’s sensorial and textural attributes. The aim of this work was to assess HPP’s effect (600 MPa/6 min and 450 MPa/6 and 9...


Study of the viability of using lipase-hydrolyzed commercial vegetable oils to ...

Fontes, Ana Luiza; Pimentel, Lígia; Soares, Ana Maria Silva; Domingues, M. Rosário; Rodríguez-Alcalá, Luis Miguel; Gomes, Ana Maria

This work studied the viability of using vegetable oils as precursor substrates to develop a dairy product enriched in microbial conjugated linoleic (CLA) and conjugated linolenic (CLNA) acids. Hydrolysis of hempseed, flaxseed (FSO) and soybean (SBO) oils was tested with Candida rugosa (CRL), Pseudomonas fluorescens, or Pancreatic porcine lipases. FSO and SBO, previously hydrolyzed with CRL, were further select...


Microbial production of terpenes

Pimentel, Lígia; Carsanba, Erdem; Teixeira, Francisca; Vidigal, Susana; Pintado, Manuela; Oliveira, Carla; Rodríguez-Alcalá, Luis M.

Sustainability is one of the main challenges facing humankind in the twenty-first century due to the continuous increase in the demand for energy and resources that characterizes our current industrial activity, driven in part by exponential population growth. This problem can be tackled from different strategies and among them the search for biomolecules, either recovered through processes based on the circula...


Perspectives on the development of CLA/CLNA-enriched milk through in situ micro...

Fontes, Ana Luiza; Pimentel, Lígia; Calzada, Javier; Salsinha, Ana Sofia; Rodríguez, Juan Miguel; Arqués, Juan; Domingues, Maria do Rosário

Over the past decade several food-derived lipids with potential bioactive properties to be used in the development of innovative functional foods have been identified. These include conjugated linoleic (CLA) and conjugated linolenic (CLNA) acids with well described anti- inflammatory, anti-obesity and anticarcinogenic properties. Limited availability in their natural sources (e.g. ruminants’ milk and meat or ve...


Impact of exposure to cold and cold-osmotic stresses on virulence-associated ch...

Alves, Ângela; Magalhães, Rui; Brandão, Teresa R. S.; Pimentel, Lígia; Rodríguez-Alcalá, Luis M.; Teixeira, Paula; Ferreira, Vânia

The objective of this work was to investigate the effect of stress conditions frequently encountered in food-associated environments on virulence-associated characteristics of eight strains of Listeria monocytogenes. Strains were grown at low (11 ºC, cold stress) and optimal (37 ºC) temperatures and in high NaCl concentrations (6% NaCl, 11 ºC; cold-osmotic stress) and tested for their ability to invade the huma...


Influence of shaking and viable cell numbers on microbial conjugated linoleic a...

Fontes, Ana Luiza; Pimentel, Lígia; Salsinha, Ana Sofia; Rodriguez, Juan Miguel; Domingues, MR; Rodríguez-Alcalá, Luís M.; Gomes, Ana Maria


Publisher Correction: Effect of Pufa Substrates on Fatty Acid Profile of Bifido...

Fontes, Ana Luiza; Pimentel, Lígia; Rodríguez-Alcalá, Luis Miguel; Gomes, Ana

The Author Contributions section in this Article contains an error. “LR, LP and AG designed the experiments, upon hypotheses conceived by LR. A.F. conducted the experimental work under supervision of L.R., L.P. and A.G. A.F., L.R. and L.P. performed statistical analysis. L.R. and L.P. accomplished data interpretation. A.F. and L.R. wrote the manuscript. L.R. and A.G. contributed to the critical review of the ma...


Lactobacillus femina sp. nov., a new species of lactobacillus delbrueckii group

Rocha, Joana; Botelho, João Francisco Tomaz Santos; Ksiezarek, Magdalena; Ribeiro, Teresa Goncalves; Machado, Miguel Paulo; Carrico, Joao Andre


One Health, Fermented Foods, and Gut Microbiota

Bell, Victoria; Ferrão, Jorge; Pimentel, Lígia; Pintado, Manuela; Fernandes, Tito

Changes in present-day society such as diets with more sugar, salt, and saturated fat, bad habits and unhealthy lifestyles contribute to the likelihood of the involvement of the microbiota in inflammatory diseases, which contribute to global epidemics of obesity, depression, and mental health concerns. The microbiota is presently one of the hottest areas of scientific and medical research, and exerts a marked i...


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