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Ultra-rapid flow cytometry assay for colistin MIC determination in Enterobacter...
Fonseca e Silva, Daniela; Andrade, Ferdinando F.; Gomes, Rosário; Silva-Dias, Ana; Martins-Oliveira, Inês; Pérez-Viso, Blanca; Ramos, Maria HelenaObjectives: Both EUCAST and CLSI recommend broth microdilution for antimicrobial susceptibility testing of colistin, but this method is cumbersome and takes 16-24 h to give results. Our objective was to evaluate a rapid quantitative colistin MIC susceptibility assay based on flow cytometry analysis (FASTcolistin MIC) in comparison with standard broth microdilution assay. Methods: One hundred and sixteen Gram-ne...
Microbes and cancer: friends or faux?
Azevedo, Maria Manuel; Pina-Vaz, Cidália; Baltazar, FátimaCancer is one of the most aggressive and deadly diseases in the world, representing the second leading cause of death. It is a multifactorial disease, in which genetic alterations play a key role, but several environmental factors also contribute to its development and progression. Infections induced by certain viruses, bacteria, fungi and parasites constitute risk factors for cancer, being chronic infection as...
Antibacterial action mechanisms of honey: physiological effects of avocado, che...
Combarros-Fuertes, Patricia; Estevinho, Leticia M.; Teixeira-Santos, Rita; Rodrigues, Acácio G.; Pina-Vaz, Cidália; Fresno, José MaríaNumerous studies have explored the antibacterial properties of di erent types of honey from all around the world. However, the data available describing how honey acts against bacteria are few. The aim of this study was to apply a flow cytometry (FC) protocol to examine and characterize the primary e ects of three varieties of honey (avocado, chestnut and polyfloral) upon physiological status of Staphylococcus ...
Evaluation of physiological effects induced by manuka honey upon staphylococcus...
Combarros-Fuertes, Patricia; Estevinho, Leticia M.; Teixeira-Santos, Rita; Rodrigues, Acácio G.; Pina-Vaz, Cidália; Fresno, José MaríaSeveral studies have explored the antimicrobial properties of manuka honey (MkH). However, the data available regarding antibacterial action mechanisms are scarcer. The aim of this study was to scrutinize and characterize primary e ects of manuka honey (MkH) upon the physiological status of Staphylococcus aureus and Escherichia coli (as Gram-positive and Gram-negative bacteria models, respectively), using flow ...
Development of cross-resistance by Aspergillus fumigatus to clinical azoles fol...
Faria-Ramos, Isabel; Farinha, Sofia; Neves-Maia, João; Tavares, Pedro Ribeiro; Miranda, Isabel M.; Estevinho, Leticia M.; Pina-Vaz, CidáliaBackground: The purpose of this study was to unveil whether azole antifungals used in agriculture, similar to the clinical azoles used in humans, can evoke resistance among relevant human pathogens like Aspergillus fumigatus, an ubiquitous agent in nature. Additionally, cross-resistance with clinical azoles was investigated. Antifungal susceptibility testing of environmental and clinical isolates of A. fumigatu...
Synergistic antimicrobial action of chlorhexidine and ozone in endodontic treat...
Noites, Rita; Pina-Vaz, Cidália; Rocha, Rita; Carvalho, Manuel Fontes; Gonçalves, Acácio; Pina-Vaz, IreneObjectives. The aim of this study was to determine whether irrigation with sodium hypochlorite, chlorhexidine, and ozone gas, alone or in combination, were effective against Enterococcus faecalis and Candida albicans; these are microorganisms frequently isolated from teeth with periapical lesions resistant to endodontic treatment. Material and Methods. 220 single root teeth, recently extracted, were inoculated ...
Environmental azole fungicide, prochloraz, can induce cross-resistance to medic...
Faria-Ramos, Isabel; Tavares, Pedro Ribeiro; Farinha, Sofia; Neves-Maia, João; Miranda, Isabel M.; Silva, Raquel M.; Estevinho, Leticia M.Acquisition of azole resistance by clinically relevant yeasts in nature may result in a significant, yet undetermined, impact in human health. The main goal of this study was to assess the development of cross-resistance between agricultural and clinical azoles by Candida spp. An in vitro induction assay was performed, for a period of 90 days, with prochloraz (PCZ) - an agricultural antifungal. Afterward, the i...
Candida albicans CUG mistranslation is a mechanism to create cell surface varia...
Miranda, Isabel; Silva-Dias, Ana; Rocha, Rita; Teixeira-Santos, Rita; Coelho, Carolina; Gonçalves, Teresa M.; Santos, Manuel A. S.; Pina-Vaz, CidáliaIn the human fungal pathogen Candida albicans, the CUG codon is translated 97% of the time as serine and 3% of the time as leucine, which potentially originates an array of proteins resulting from the translation of a single gene. Genes encoding cell surface proteins are enriched in CUG codons; thus, CUG mistranslation may influence the interactions of the organism with the host. To investigate this, we compare...
Determination of chitin content in fungal cell wall: an alternative flow cytome...
Costa-de-Oliveira, Sofia; Silva, Ana P.; Miranda, Isabel M.; Salvador, Alexandre; Azevedo, Maria M.; Munro, Carol A.; Rodrigues, Acácio G.The conventional methods used to evaluate chitin content in fungi, such as biochemical assessment of glucosamine release after acid hydrolysis or epifluorescence microscopy, are low throughput, laborious, time-consuming, and cannot evaluate a large number of cells. We developed a flow cytometric assay, efficient, and fast, based on Calcofluor White staining to measure chitin content in yeast cells. A staining i...
Candida albicans CUG mistranslation is a mechanism to create cell surface varia...
Miranda, Isabel; Silva-dias, Ana; Rocha, Rita; Teixeira-Santos, Rita; Coelho, Carolina; Gonçalves, Teresa; Santos, Manuel A. S.; Pina-Vaz, CidáliaIn the human fungal pathogen Candida albicans, the CUG codon is translated 97% of the time as serine and 3% of the time as leucine, which potentially originates an array of proteins resulting from the translation of a single gene. Genes encoding cell surface proteins are enriched in CUG codons; thus, CUG mistranslation may influence the interactions of the organism with the host. To investigate this, we compare...