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Density separation of petrous bone powders for optimized ancient DNA yields

Fernandes, Daniel; Sirak, Kendra A.; Cheronet, Olivia; Novak, Mario; Brück, Florian; Zelger, Evelyn; Llanos-Lizcano, Alejandro; Wagner, Anna

Density separation is a process routinely used to segregate minerals, organic matter, and even microplastics, from soils and sediments. Here we apply density separation to archaeological bone powders before DNA extraction to increase endogenous DNA recovery relative to a standard control extraction of the same powders. Using nontoxic heavy liquid solutions, we separated powders from the petrous bones of 10 indi...


A genetic history of continuity and mobility in the Iron Age central Mediterranean

Moots, Hannah M.; Antonio, Margaret; Sawyer, Susanna; Spence, Jeffrey P.; Oberreiter, Victoria; Weiß, Clemens L.; Lucci, Michaela

The Iron Age was a dynamic period in central Mediterranean history, with the expansion of Greek and Phoenician colonies and the growth of Carthage into the dominant maritime power of the Mediterranean. These events were facilitated by the ease of long-distance travel following major advances in seafaring. We know from the archaeological record that trade goods and materials were moving across great distances in...


"Dar rosto à Villa" - Projecto de aproximação facial em torno de um esqueleto d...

Dias, Vanessa; Encarnação, Gisela; Quintino Arias, Yuliet; Fernandes, Daniel; Franco, Filipe; Pinhasi, Ron; Conejo Delgado, Noé; Oliveira, César

Tendo por base vários procedimentos científicos, tais como, a análise antropológica e arqueológica pormenorizada, a leitura do genoma a partir das análises de ADN antigo preservado e a datação pelo método de radiocarbono, a ilustração anatómica do rosto foi ganhando forma, revelando uma mulher entre os 35 e os 45 anos de idade, com cabelos claros e pele ligeiramente morena que, como a cronologia e proximidade s...


Genomes from Verteba cave suggest diversity within the Trypillians in Ukraine

Gelabert, Pere; Schmidt, Ryan W; Fernandes, Daniel; Karsten, Jordan K; Harper, Thomas K; Madden, Gwyn D; Ledogar, Sarah H; Sokhatsky, Mykhailo

The transition to agriculture occurred relatively late in Eastern Europe, leading researchers to debate whether it was a gradual, interactive process or a colonisation event. In the forest and forest-steppe regions of Ukraine, farming appeared during the fifth millennium BCE, associated with the Cucuteni-Trypillia cultural complex (CTCC, ~ 5000-3000 BCE). Across Europe, the Neolithisation process was highly var...


An integrative skeletal and paleogenomic analysis of stature variation suggests...

Marciniak, Stephanie; Bergey, Christina M.; Silva, Ana Maria; Hałuszko, Agata; Furmanek, Mirosław; Veselka, Barbara; Velemínský, Petr

Human culture, biology, and health were shaped dramatically by the onset of agriculture ∼12,000 y B.P. This shift is hypothesized to have resulted in increased individual fitness and population growth as evidenced by archaeological and population genomic data alongside a decline in physiological health as inferred from skeletal remains. Here, we consider osteological and ancient DNA data from the same prehistor...


Ancient DNA and deep population structure in sub-Saharan African foragers

Lipson, Mark; Sawchuk, Elizabeth A.; Thompson, Jessica C.; Oppenheimer, Jonas; Tryon, Christian A.; Ranhorn, Kathryn L.; de Luna, Kathryn M.

Multiple lines of genetic and archaeological evidence suggest that there were major demographic changes in the terminal Late Pleistocene epoch and early Holocene epoch of sub-Saharan Africa(1-4). Inferences about this period are challenging to make because demographic shifts in the past 5,000 years have obscured the structures of more ancient populations(3,5). Here we present genome-wide ancient DNA data for si...


Integrating buccal and occlusal dental microwear with isotope analyses for a co...

Hernando, Raquel; Gamarra, Beatriz; McCall, Ashley; Cheronet, Olivia; Fernandes, Daniel; Sirak, Kendra; Schmidt, Ryan; Lozano, Marina; Szeniczey, Tamás

Dietary reconstruction is used to make inferences about the subsistence strategies of ancient human populations, but it may also serve as a proxy to characterise their diverse cultural and technological manifestations. Dental microwear and stable isotope analyses have been shown to be successful techniques for paleodietary reconstruction of ancient populations but, despite yielding complementary dietary informa...


Social stratification without genetic differentiation at the site of Kulubnarti...

Sirak, Kendra A; Fernandes, Daniel; Lipson, Mark; Mallick, Swapan; Mah, Matthew; Olalde, Iñigo; Ringbauer, Harald; Rohland, Nadin; Hadden, Carla S

Relatively little is known about Nubia's genetic landscape prior to the influence of the Islamic migrations that began in the late 1st millennium CE. Here, we increase the number of ancient individuals with genome-level data from the Nile Valley from three to 69, reporting data for 66 individuals from two cemeteries at the Christian Period (~650-1000 CE) site of Kulubnarti, where multiple lines of evidence sugg...


TKGWV2: an ancient DNA relatedness pipeline for ultra-low coverage whole genome...

Fernandes, Daniel; Cheronet, Olivia; Gelabert, Pere; Pinhasi, Ron

Estimation of genetically related individuals is playing an increasingly important role in the ancient DNA field. In recent years, the numbers of sequenced individuals from single sites have been increasing, reflecting a growing interest in understanding the familial and social organisation of ancient populations. Although a few different methods have been specifically developed for ancient DNA, namely to tackl...



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