Mucosal T lymphocytes from patients with ulcerative colitis (UC) were previously shown to display a deficiency in branched N-glycosylation associated with disease severity. However, whether this glycosylation pathway shapes the course of the T cell response constituting a targeted-specific mechanism in UC remains largely unknown. In this study, we demonstrated that metabolic supplementation of ex vivo mucosal T...
Studies of chemokine receptors (CKR) in natural killer- (NK-) cells have already been published, but only a few gave detailed information on its differential expression on blood NK-cell subsets. We report on the expression of the inflammatory and homeostatic CKR on normal blood CD56(+low) CD16(+) and CD56(+high) CD16(-/+low) NK-cells. Conventional CD56(+low) and CD56(+high) NK-cells present in the normal PB do...
Background Circulating endothelial cells (CEC) may be a biomarker of vascular injury and pro-thrombotic tendency, while circulating endothelial progenitor cells (CEP) may be an indicator for angiogenesis and vascular remodelling. However, there is not a universally accepted standardized protocol to identify and quantify these cells and its clinical relevancy remains to be established. Objectives To quantify CEC...
MATURAÇÃO DA LINHA ERITRÓIDE NA MEDULA ÓSSEA POR CITOMETRIA DE FLUXO Maria Luís Queirós1,2,3, Liliana Cerejo1,4, Magdalena Leander1,3, Inês Freitas3,5, Esmeralda Cleto3,6, José Barbot3,7, Marta Gonçalves1,3, Marlene Santos1,3, Ana Helena Santos1,3,8, Sónia Fonseca1,3, Catarina Lau1,3, Maria Anjos Teixeira1,3, Luciana Pinho1,3, Alice Santos-Silva2, Margarida Lima1,3,8 1Serviço Hematologia Clínica, Laboratório de...
Os reticulócitos são eritrócitos jovens cuja presença no sangue periférico (SP) reflecte a actividade eritropoiética da medula óssea. Assim e apesar de, em determinadas situações de stress ou em diversas patologias hematológicas, se poderem encontrar no SP reticulócitos em diferentes estadios de maturação,pouco se sabe acerca deles. Este trabalho teve como objectivo caracterizar fenotipicamente as várias fases ...