119 documents found, page 1 of 12
Mechanical characterization of blood cells: microfluidics technology
Gonçalves, Margarida José Sousa; Melo-Fonseca, Francisca; Pinto, Inês Mendes; Catarino, Susana Oliveira; Pinho, DianaMechanical cell alterations, such as changes in stiffness and morphology, play a significant role in several blood diseases. Therefore, targeting cells’ physical properties presents opportunities for intervention and can help in the development of novel diagnoses and therapeutic strategies. The lack of clinical relevance of such biophysical parameters is due to the very low measurement throughput and tedious op...
Polydimethylsiloxane surface modification for microfluidic devices and organ-on...
Gonçalves, Inês Maia Silva Rodrigo; Gonçalves, Margarida; Pinho, Diana; Cardoso, Beatriz Dias; Sousa, Patrícia C.; Zille, Andrea; Hori, TakeshiOne of the most popular materials for creating bio models and microfluidic devices is polydimethylsiloxane (PDMS). Recently, a unique class of devices called organs-on-a-chip (OoC) was created by fusing cell cultures with microfluidic devices. To make it easier to investigate both healthy and pathological conditions, these devices replicate the micro physiological characteristics of the human body. OoCs are mos...
A multi Organ-on-chip platform for new nanocarriers studies: numerical and expe...
Gonçalves, Inês Maia Silva Rodrigo; Gonçalves, Margarida José Sousa; Minas, Maria Manuela Henriques; Lima, Rui Alberto Madeira Macedo; Pinho, DianaIt is widely accepted that organ-on-chip systems will have a significant impact on the future of the pharmaceutical and biotechnology industry through the discovery of new drugs using robust in vitro platforms that will complement or potentially replace the current pre-clinical animal studies [1–5]. In this work, it is proposed an innovative and robust microfluidic multi-OoC platform, to support the 3D biologic...
Modeling microgravity using clinorotation in a microfluidic environment: a nume...
Fernandes, João; Machado, Dara; Minas, Graça; Catarino, Susana Oliveira; Pinho, DianaMicrogravity simulation is essential for studying particle dynamics in space-related applications where traditional gravitational effects are absent. This study presents a numerical investigation of particle behavior in a clinostat-driven microfluidic channel, aiming to simulate microgravity conditions. A computational model was developed in COMSOL Multiphysics to analyze the impact of channel size, particle di...
Evaluating the response of malaria-infected RBCs to magnetic nanocarriers under...
Ferreira, Margarida Gomes; Freitas, Bruno José Ferreira; Baptista, Vitória Cunha; Pinho, Diana; Minas, Graça; Veiga, Maria IsabelMalaria still affects millions of people each year, posing a persistent public health challenge. One major hurdle is the increasing resistance of malaria parasite, Plasmodium falciparum, to the current therapeutic drugs, which calls for alternative treatment strategies. This work takes a first step in exploring whether targeted drug delivery systems using nanocarriers can provide a promising solution to mitigat...
Long-term hydrophilic PDMS surfaces using Brij L4: A simple and robust approach...
Minas, Graça; Pinho, Diana; Borges, Joel; Vaz, Filipe; Zille, Andrea; Lima, Rui Alberto Madeira MacedoPolydimethylsiloxane (PDMS) is a widely used material in the production of microfluidic devices and biomodels, due to its exceptional mechanical, chemical and biocompatible properties. Recently, a novel class of devices known as microphysiological systems have emerged, combining microfluidic technology and cell culture, mimicking the microphysiological features of the human body to facilitate the study of both ...
PDMS surface wettability modification and its applications: a systematic review
Ramos, Inês; Gonçalves, Margarida José Sousa; Gonçalves, Inês Maia Silva Rodrigo; Carvalho, Violeta Meneses; Fernandes, ElisabetePolydimethylsiloxane (PDMS) exhibits unique properties that make it essential in various biomedical applications. This systematic review critically examines current research on PDMS surface modification methods, focusing on enhancing its biocompatibility and functionality. Following a rigorous selection process aligned with PRISMA guidelines, 83 relevant studies published between 2013 and July 2024, out of 938 ...
Capillary dynamics analysis of millimetric tubes for anti-slip surfaces: A phas...
Sharma, Shivam; Richhariya, Vipin; Pinho, Diana; Carvalho, Óscar Samuel Novais; Silva, Filipe Samuel; Tripathy, Ashis; Dourado, N.The entrance and viscous flow zones of capillary dynamics are critical in anti-slip surfaces. Here we propose the numerical modelling of capillary filling, based on the phase-field method, incorporating dynamic contact angle and wetted wall boundary conditions. The entrance and inertial effects of single-phase water rising naturally in a millimeter-sized vertical cylinder are demonstrated. The numerical approac...
Development and fabrication of a microfluidic device for mechanical characteriz...
Ferreira, Margarida Gomes; Freitas, Bruno; Baptista, Vitória; Pinho, Diana; Minas, Graça; Veiga, Maria Isabel; Faustino, Vera; Catarino, Susana OliveiraThis work reports the design and fabrication of a polymeric microfluidic device, with a hyperbolic contraction, to promote extensional flow and allow the single cell mechanical characterization, in vitro, of healthy and malaria-infected red blood cells (RBCs). The obtained results show an extensional flow profile of the velocity across the microchannel for both healthy and infected RBCs, while the ability of th...
Polydimethylsiloxane surface modification of microfluidic devices for blood pla...
Gonçalves, Margarida; Gonçalves, Inês Maia; Borges, Joel; Faustino, Vera Luísa Carreira; Soares, Delfim; Vaz, Filipe; Minas, GraçaOver the last decade, researchers have developed a variety of new analytical and clinical diagnostic devices. These devices are predominantly based on microfluidic technologies, where biological samples can be processed and manipulated for the collection and detection of important biomolecules. Polydimethylsiloxane (PDMS) is the most commonly used material in the fabrication of these microfluidic devices. Howev...