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Molecular and serological screening support the lack of Coxiella burnetii circu...

Loureiro, Filipa; Mesquita, João R.; Cardoso, Luis; Pintado, Cristina Santos; Gonçalves, S. Gomes; Matos, Ana; Soeiro, V.; Gonçalves, A.; Silva, Filipe

Coxiella burnetii is a highly infectious zoonotic pathogenic bacterium that has a major economic impact in the livestock industry throughout the world and causes unpredictable outbreaks in humans worldwide. Although it is known that birds are potential reservoirs of C. burnetii, their role in the epidemiological cycle of the pathogen has not been fully verified. Due to its non-specific symptoms and clinical sig...


Spectroscopic insights into prodigiosin pigment production by Serratia marcescens

Ferraz, Ana Rita Simões; Fernandes, Mariana; Goulão, Manuela; Pintado, Cristina Santos; Anjos, O.

The appearance of cheese can be influenced by the intentional use of dyes such as paprika that color its rind, particularly in smear-ripened cheeses. In addition to this intentional addition, some microorganisms naturally present in cheeses can produce pigments that lead to color defects that negatively impact consumer acceptance and economic profitability. Serratia marcescens can produce a red pigment known as...


Revestimentos comestíveis ativos para prevenir defeitos de cor no queijo

Ferraz, Ana Rita Simões; Pintado, Cristina Santos; Anjos, O.

O queijo é um alimento vivo, considerado um ecossistema bio complexo colonizado por um grupo diversificado de microrganismos, conhecido como microbiota do queijo, proveniente do leite cru, culturas iniciadoras e culturas adjuntas. Esses microrganismos são os principais contribuintes para os atributos sensoriais percebidos de diferentes queijos devido à sua complexa interação com proteínas do leite, carboidratos...


Is Coxiella burnetii circulating in wild birds in the North of Portugal?

Loureiro, Filipa; Cardoso, Luís; Matos, Ana Cristina; Pintado, Cristina Santos; Silva, Filipe; Matos, Manuela; Coelho, Ana Cláudia

Coxiella burnetii is the causative agent of Q fever, a zoonosis of important public health concern, and a disease that has a serious economic impact on the production of farm animals. The role of birds as vectors of bacteria and other pathogens to farm animals has been suggested, thorough environmental contamination of pasturelands, water supplies and feed by avian drops. Migratory birds might also be the mean ...


Hunting for answers: Assessing Brucella spp. seroprevalence and risks in red de...

Pires, Humberto; Cardoso, Luís; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Silva, Sérgio Santos; Matos, Manuela; Pintado, Cristina Santos

Between 2016 and 2023, a cross-sectional study was conducted in the central region of Portugal in order to better understand the epidemiology and public health risks resulting from the handling and consumption of game animals infected with Brucella spp. The seroprevalence and risk factors for Brucella spp. seropositivity were evaluated. Antibodies against Brucella spp. were determined using a commercial enzyme-...


Serological evidence of West Nile virus in wild birds in Portugal

Loureiro, Filipa; Matos, Ana Cristina; Pintado, Cristina Santos; Cardoso, Luís; Silva, Filipe; Matos, Manuela; Coelho, Ana Cláudia

West Nile virus (WNV) has a zoonotic transmission cycle. Avian species are considered the definitive hosts, and mosquitoes and other arthropods are the main vectors. Humans are considered accidental and dead-end hosts, since mammals are unable to efficiently amplify the virus. We aimed at studying serological prevalence of WNV circulation in wild birds in Portugal. Three hundred and six individuals belonging to...


Serological evidence of antibodies to Flaviviridae in wild birds in Portugal

Loureiro, Filipa; Cardoso, Luís; Matos, Ana Cristina; Pintado, Cristina Santos; Silva, Filipe; Ferreira, Mariana; Brandão, Ricardo; Lopes, Carolina

Emerging infectious diseases are a major threat to biodiversity and an important public health issue. Flaviviruses are the cause of several emerging vector-borne zoonotic arboviruses whose distribution is currently increasing in Europe. The evidence that West Nile virus (WNV) circulates in resident and migratory species has implications for both animal and public health and should therefore be studied in depth....


A global review of cheese colour: microbial discolouration and innovation oppor...

Ferraz, Ana Rita Simões; Pintado, Cristina Santos; Serralheiro, Maria Luísa

Cheese is a biologically active food product, characterised by its colour, texture, and taste. Due to its rich matrix of fats and proteins, as well as the fact that the cheese’s surface acts as its own packaging, the cheese becomes more susceptible to contamination by microorganisms during the ripening process, particularly bacteria and fungi. The ripening of cheese involves several biochemical reactions, with ...


Seropositivity for Coxiella burnetii in wild boar (Sus scrofa) and red deer (Ce...

Pires, Humberto; Cardoso, Luís; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Matos, Manuela; Pintado, Cristina Santos; Figueira, Luís

Q fever is caused by the pathogen Coxiella burnetii and is a zoonosis that naturally infects goats, sheep, and cats, but can also infect humans, birds, reptiles, or arthropods. A survey was conducted for the detection of antibodies against C. burnetii in a sample of 617 free-ranging wild ruminants, 358 wild boar (Sus scrofa) and 259 red deer (Cervus elaphus), in east–central Portugal during the 2016–2022 huntin...


Prevalence and risk factors for hepatitis E virus in wild boar and red deer in ...

Pires, Humberto; Cardoso, Luís; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Silva, Sérgio Santos; Matos, Manuela; Pintado, Cristina Santos

Hepatitis E virus (HEV) is a zoonotic foodborne virus with an annual infection prevalence of 20 million human cases, which seriously affects public health and economic development in both developed and developing countries. To better understand the epidemiology of HEV in Central Portugal, a cross-sectional study was conducted from 2016 to 2023 with sera samples from wild ungulates. The seroprevalence and risk f...


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